Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (15): 3115-3124 .doi: 10.3864/j.issn.0578-1752.2010.15.008

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and mRNA Expression Levels of Cytochrome P450 Genes CYP4M14 and CYP4S9 in the Common Cutworm Spodoptera litura (Fabricius)

HUANG Shui-jin, QIN Wen-jing, CHEN Qiong
   

  1. (江西省农业科学院植物保护研究所)
  • Received:2009-12-28 Revised:2010-06-08 Online:2010-08-01 Published:2010-08-01

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Abstract:

【Objective】 Molecular mechanism of deltamethrin resistance in the common cutworm was studied. 【Method】Two novel P450 genes (CYP4M14 and CYP4S9) of Spodoptera litura were cloned using the RT-PCR with degenerate primers and rapid amplification of cDNA ends (RACE) strategies, and mRNA expression levels of CYP4M14 and CYP4S9 were compared between the resistant and susceptible strain, respectively. 【Result】 The coding region of CYP4M14 cDNA was 1 512 bp, encoding a 504-amino-acid protein, while that of CYP4S9 cDNA was 1 473 bp, encoding a 491-amino-acid protein. Relative mRNA expression levels of CYP4M14 and CYP4S9 in the resistant strain were compared with that in the susceptible strain using real-time quantitative PCR. It was found that CYP4M14 and CYP4S9 in the resistant strain were expressed 4.85 and 18.63 fold higher in the midgut and 75.14 and 6.07 fold in the fatbody, respectively. 【Conclusion】 Constitutive overexpression of CYP4M14 and CYP4S9 may likely contribute to deltamethrin resistance in S. litura.

Key words: Spodoptera litura, cytochrome P450, real-time quantitative PCR, P450 genes

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