Abstract:

【Objective】 The rotenone content of Tephrosia vogelii Hook was low in original strains and the plants cultivated by the seeds from space flight had been screened for high content of rotenone. 【Method】 The chosen seeds of Tephrosia vogelii carried by the recoverable satellite had been travelled for 15 days in space, then the seeds were sowed in the field after recovery. The qualitative and quantitative analyses on rotenone content of leaves powder of 150 Tephrosia vogelii plants were conducted by HPLC and HPLC-MS. 【Result】 Two plants with high rotenone content in the powder which was more than 2.00% and eight plants with the rotenone content of 1.00%-2.00% in the germplasm were oblained. No.21 plant had the highest rotenone content up to 2.3172% in all Tephrosia vogelii germplasm resources which was 4.02 times higher than the original strains with rotenone content only 0.4614%. 【Conclusion】High content rotenone specific resources of Tephrosia vogelii from recoverable satellite can be used as important materials for rotenone extraction after genetic improvement or massive reproduction.

Key words: space flight, rotenone, content, screening, HPLC