Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (12): 2333-2345.doi: 10.3864/j.issn.0578-1752.2025.12.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATIONTECHNOLOGY • Previous Articles     Next Articles

Effects of Multiple Cropping Green Manure Combined with Different Nitrogen Fertilizer Levels on Yield and Quality of Spring Wheat

LI Rong1(), LI ZhengPeng1, YAN QingBiao1, GUO RanRan2, HAN Mei1(), XU Ke2()   

  1. 1 Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016
    2 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016
  • Received:2025-01-17 Accepted:2025-04-17 Online:2025-06-19 Published:2025-06-19
  • Contact: HAN Mei, XU Ke

Abstract:

【Objective】This study aimed to explore the effects of multiple cropping green manure combined with different levels of nitrogen fertilizer on the yield and grain quality of spring wheat, so as to provide the theoretical guidance for the construction of chemical fertilizer reduction and high-quality production mode based on green manure in Qinghai province.【Method】The split plot experiment was carried out in the experimental site of the Academy of Agricultural and Forestry Sciences of Qinghai University from 2023 to 2024. Two planting patterns were set up in the main area: multiple cropping green manure after wheat (W-G) and leisure after wheat (W), and three nitrogen fertilizer levels in the sub-area included: no nitrogen application (N0), nitrogen fertilizer reduction by 30% (N1, 157.5 kg N·hm-2), and the local custom of nitrogen application (N2, 225 kg N·hm-2). Wheat yield and grain quality (grain protein content, sedimentation value, formation time, stability time, wet gluten, etc.) were determined.【Result】The grain yield of spring wheat under W-GN1 treatment was 5.5% and 13.4% higher than that under WN1 in 2023 and 2024, and 2.0% and 5.3% higher than that under WN2 treatment, respectively; the biological yield under W-GN1 in 2023 and 2024 was 5.1% and 10.6% higher than that under WN1, and 1.5% and 4.6% higher than that under WN2, respectively. W-G could obtain higher harvest index than W. The compensation effect of multiple cropping green manure was negative under no nitrogen application level, and it was between 2.0%-14.0% under nitrogen application level. The contribution of multiple cropping green manure combined with 30% reduction of nitrogen fertilizer to increasing crop yield was the best. At the same time, W-GN1 could improve grain quality by increasing grain protein content, sedimentation value, formation time and stabilization time. The grain protein content under W-GN1 treatment was 10.62% and 9.48% higher than that under WN1, respectively. The grain sedimentation value increased by 25.05% and 18.13%, respectively. The grain formation time increased by 34.70% and 8.66%, respectively. The grain stability time increased by 41.30% and 13.68%, respectively. Through principal component analysis, it demonstrated that the multiple cropping of green manure after wheat had a more significant promoting effect in grain protein content, sedimentation value, formation time and stability time.【Conclusion】The grain yield and quality of spring wheat were significantly improved by 30% reduction of nitrogen fertilizer (N1,157.5 kg N·hm-2) combined with green manure after wheat harvest, which could be used as a suitable planting mode and nitrogen application level for improving quality and stable yield of wheat under the condition of reducing chemical fertilizer in Qinghai Province.

Key words: green manure, nitrogen level, yield, grain quality, spring wheat

Fig. 1

Precipitation and temperature from March to July in 2023 and 2024"

Table 1

Treatment combination codes and specific measures under different cropping patterns and nitrogen levels"

种植模式
Cropping pattern
施氮水平
Nitrogen level
处理组合代码
Treatment combined code
具体措施
Specific measure
麦后复种绿肥
Wheat multiple cropping with green manure(W-G)
不施氮 No nitrogen (N0) W-GN0 小麦收获后浅旋复种绿肥,在10月中旬落霜后(绿肥盛花期),将绿肥全量翻压还田,翻压深度25 cm
After wheat harvest, shallow rotation stubble killing and replanting of green manure were carried out. After frost fell in mid-October (the green manure flowering period), the full amount of green manure was turned over and returned to the field, with a turning depth of 25 cm
氮肥减施30%
Nitrogen fertilizer was reduced by 30% (N1)
W-GN1
当地习惯施氮
The local custom of nitrogen (N2)
W-GN2
麦后休闲
Wheat with autumn fallow(W)
不施氮 No nitrogen (N0) WN0 小麦收获后夏休闲,在10月中旬落霜后,与复种绿肥处理同时进行耕翻,耕翻深度25 cm
Summer leisure after wheat harvest, after frost fell in mid- October, ploughing was carried out at the same time as green manure treatment,and the ploughing depth was 25 cm
氮肥减施30%
Nitrogen fertilizer was reduced by 30% (N1)
WN1
当地习惯施氮
The local custom of nitrogen (N2)
WN2

Table 2

Wheat and green manure planting in 2023 and 2024"

试验年份
Test year
作物
Crop plant
品种
Variety
播种日期
Sowing
date
收获日期
Harvest
date
翻压日期
Overturn
date
播种量
Sowing rate (kg·hm-2)
播种方式
Sowing method
2023 小麦
Wheat
青春38号
Qingchun 38
03-27 08-02 - 300 条播,行距16cm
Drill-seeded at 16 cm row spacing
绿肥
Green manure
土库曼毛叶苕子
Turkmen Vicia villosa
08-07 - 10-27 150 撒播
Broadcast sowing
2024 小麦
Wheat
青春38号
Qingchun 38
03-18 07-27 - 300 条播,行距16cm
Drill-seeded at 16 cm row spacing
绿肥
Green manure
土库曼毛叶苕子
Turkmen Vicia villosa
07-30 - 10-29 150 撒播
Broadcast sowing

Table 3

Nitrogen level of wheat"

施氮水平
Nitrogen level
后茬小麦尿素用量Urea amount of wheat (kg N·hm-2)
基肥 Basal manure 四叶期追肥 Four leaf topdressing
不施氮 No nitrogen (N0) 0 0
氮肥减施30% Nitrogen fertilizer was reduced by 30% (N1) 275 68.50
当地习惯施氮 The local custom of nitrogen (N2) 390 97.85

Fig. 2

Wheat yield and harvest index under different cropping patterns and nitrogen levels"

Table 4

Contribution rate and compensation effect under different cropping patterns and nitrogen levels"

种植模式
Cropping pattern
施氮水平
Nitrogen level
绿肥贡献率
GMCR (%)
氮肥贡献率
NCR (%)
肥料贡献率
FCR (%)
补偿效应
Compensation effect (%)
2023 W-G N0 38.1 - 38.1 -25.6
N1 5.2 49.6 54.8 2.0
N2 12.3 47.3 59.6 14.0
W N0 - - - -
N1 - 52.3 52.3 -
N2 - 53.9 53.9 -
2024 W-G N0 39.8 - 39.8 -45.2
N1 11.8 56.8 68.6 5.3
N2 8.5 61.3 69.8 9.3
W N0 - - - -
N1 - 64.4 64.4 -
N2 - 67.0 67.0 -

Table 5

Effects of cropping patterns and nitrogen levels on wheat grain quality"

种植模式
Cropping pattern
施氮水平
Nitrogen level
蛋白
Grain protein (%)
沉降值
Sedimentation value (mL)
形成时间Formation time (min) 稳定时间
Stability time (min)
容重
Unit weight(g·L-1
湿面筋
Wet gluten (%)
吸水率
Water absorption (%)
硬度指数
Hardness index (%)
出粉率
Flour yield (%)
2023 W-G N0 13.94b 37.47a 3.63c 13.13b 781.67c 28.52b 65.13a 76.33a 73.73a
N1 15.00a 39.27a 4.33a 14.23a 810.00a 31.60a 65.03a 76.67a 73.47a
N2 14.79a 40.87a 3.97b 14.03a 810.67a 31.73a 65.40a 78.00a 76.33a
W N0 12.36c 25.97c 2.67e 10.67c 780.67c 25.75c 62.87a 73.67a 75.03a
N1 13.56b 31.40b 3.07d 10.57c 798.67b 29.43b 65.50a 76.67a 71.63a
N2 14.60a 32.47b 3.43c 11.30c 807.33a 31.47a 64.03a 73.33a 72.97a
2024 W-G N0 13.39b 38.70c 3.30ab 12.27b 792.33b 27.29b 61.37a 71.00a 73.17ab
N1 14.48a 41.70b 3.60a 13.80a 804.33a 29.89a 63.57a 75.00a 75.40a
N2 14.55a 45.67a 3.57a 13.57a 808.33a 30.33a 63.63a 74.67a 75.73a
W N0 12.31c 33.17e 2.63c 11.53c 788.00b 25.15c 57.93b 63.67b 70.17b
N1 13.23b 35.30d 3.17b 12.70b 801.00a 27.66b 62.33a 73.33a 76.97a
N2 13.56b 39.50c 3.37ab 12.77b 801.67a 28.42b 63.30a 74.33a 77.03a
种植模式
Cropping pattern (C)
*** *** *** *** ** *** NS NS NS
施氮水平
Nitrogen level (N)
*** *** *** ** *** *** * * NS
C×N NS NS * NS NS NS NS NS NS

Fig. 3

Correlation analysis of wheat grain quality index"

Table 6

Principal component analysis characteristic value and load change table"

成分
Component
初始特征值 Initial eigenvalue 提取载荷平方和 Extraction eigenvalue 旋转载荷平方和 Rotation eigenvalue
总计
Total
方差百分比
Variance
(%)
累积
Cumulative (%)
总计
Total
方差百分比
Variance percentage (%)
累积
Cumulative (%)
总计
Total
方差百分比
Variance percentage (%)
累积
Cumulative (%)
1 5.124 56.936 56.939 5.124 56.936 56.936 3.979 44.215 44.215
2 1.445 16.058 72.994 1.445 16.058 72.994 2.389 26.550 70.765
3 1.098 12.202 85.196 1.098 12.202 85.196 1.299 14.431 85.196
4 0.625 6.949 92.145 - - - - - -
5 0.392 4.353 96.497 - - - - - -
6 0.159 1.772 98.269 - - - - - -
7 0.080 0.892 99.161 - - - - - -
8 0.047 0.518 99.679 - - - - - -
9 0.029 0.321 100.000 - - - - - -

Table 7

Component matrix of wheat grain quality index"

指标
Index
初始成分矩阵 Initial component matrix 旋转后成分矩阵 Rotation component matrix
PC1 PC2 PC3 PC1 PC2 PC3
蛋白Grain protein 0.905 -0.139 -0.272 0.853 0.422 -0.089
沉降值Sedimentation value 0.706 -0.495 0.230 0.838 -0.103 0.291
形成时间Formation time 0.905 -0.139 -0.038 0.832 0.360 0.137
稳定时间Stability time 0.794 -0.342 0.356 0.815 0.035 0.457
容重Unit weight 0.738 -0.226 -0.181 0.754 0.241 -0.052
湿面筋Wet gluten 0.886 0.040 -0.308 0.741 0.568 -0.097
吸水率Water absorption 0.659 0.654 -0.217 0.207 0.930 0.047
硬度指数Hardness index 0.674 0.700 0.124 0.163 0.886 0.386
出粉率Flour yield 0.372 0.271 0.808 0.087 0.201 0.903
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