Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4335-4342.doi: 10.3864/j.issn.0578-1752.2012.21.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Studies on Mapping and Inheritance of Resistance Genes to SMV Strain SC10 in Soybean

 LI  Chun-Yan, YANG  Yong-Qing, WANG  Da-Gang, LI  Hua-Wei, ZHENG  Gui-Jie, WANG  Tao, ZHI  Hai-Jian   

  1. 1.南京农业大学农学院/作物遗传与种质创新国家重点实验室/农业部大豆生物学与遗传育种重点实验室/国家大豆改良中心，南京 210095
  • Received:2012-06-20 Online:2012-11-01 Published:2012-08-31

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Abstract: 【Objective】The inheritance of resistance and allelism of resistance genes to SMV strain SC10 was studied and the resistance genes to SC10 in Kefeng No.1 were mapped.【Method】The resistant soybean cultivars to SMV strain SC10(Kefeng No.1, Jinda74, Dabaima, Fendou56, Zhongzuo229, Xudou No.1, Pixianchadou, Kwanggyo and Yuejin No.4)were crossed with susceptible (S) cultivars (Nannong 1138-2 or 8101), respectively, to determine the inheritance of resistance to SC10. Each R parents were also crossed with each other to test the allelism of the resistance genes. The bulked segregant analysis (BSA) of SSR markers and F2 population from Kefeng No.1 × Nannong 1138-2 were used in mapping the resistance gene.【Result】The F1 of crosses from resistant cultivars (Kefeng No.1, Jinda74, Dabaima, Fendou56, Zhongzuo229, Xudou No.1) and susceptible (S) cultivars (Nannong 1138-2 or 8101) were resistant, F2 segregated in 3 resistant﹕1 susceptible ratio, and 1R﹕2Seg﹕1S in F2:3 families. The F1 and F2 populations from Jinda74×Fendou56 and Xudou No.1×Pixianchadou exhibited complete resistant. F1 from the two crosses of Kefeng No.1×Kwanggyo, Fendou56 and Zhongzuo229, Jinda74×Zhongzuo229 and Kwanggyo, Dabaima×Fendou56 and Kefeng No.1. Yuejin No.4×Kwanggyo were all resistant while their correspond F2 population segregated in a ratio of 15(R)﹕1(S). The seven SSR markers (Satt558, Sat_254, Satt634, Gm020580, Gm020584, Gm020562 and Gm020546) were found linking to RSC10. The genetic distance between the resistance gene RSC10 and SSR markes were 6.1, 2.0, 0.9, 0.8, 2.0, 4.6, and 9.2 cM, respectively.【Conclusion】 Kefeng No.1, Jinda74, Dabaima, Xudou No.1, Fendou 56, Zhongzuo229, respectively, have a single dominant gene controlling the resistance to SC10. The resistance genes in Jinda74 and Fendou 56, Xudou No.1 and Pixianchadou were at a same locus or very closely linked. It was inferred that the resistance genes between Kefeng No.1 and Kwanggyo, Fendou56, Zhongzuo229; between Jinda74 and Zhongzuo229, Kwanggyo; between Dabaima and Fendou56, Kefeng No.1; between Yuejin No.4 and Kwanggyo were not at the same locus. The resistance gene carried by Kefeng No.1 to SC10 was located on D1b linkage group.

Key words: soybean, soybean mosaic virus, resistance, gene mapping

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