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Proteome Analysis of Small Heat Shock Proteins in Bombyx mori Testis at Different Developmental Stages

NIE Hong-yi, ZHONG Xiao-wu, YI Qi-ying, ZHANG Li-ping, ZOU Yong, ZHAO Ping   

  1. The Key Sericulture Laboratory of Ministry of Agriculture, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716
  • Received:2010-08-02 Online:2011-05-05 Published:2010-12-07

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Abstract: 【Objective】 The objective of this study is to investigate the developmental changes in the expression of major small heat shock proteins (sHSP) in Bombyx mori testis and to provide a theoretical basis for the molecular mechanism of sHSPs in spermatogenesis.【Method】 The electronphoretogram of testis from day 5 of fifth instar, day 1 of pupa and day 1 moth were established by two-dimensional polyacrylamide gel electrophoresis. Moreover, Imagemaster2D 6.0 was performed to analyze the different expression patterns of these sHSPs and these proteins were identi?ed by matrix-assistedlaser desorption/ionization mass spectrometry (MALDI-TOF-MS). 【Result】 Five sHSPs (sHSP23.7, sHSP20.8, sHSP20.4, sHSP19.9 and sHSP19.8) were successfully identified on two-dimensional gels. And it was found that the level of protein biosyntesis of these five sHSPs in the day 1 of pupa and day 1 moth reduced so obviously compared with the day 5 of fifth instar. 【Conclusion】 Since the day 5 of fifth instar is the important period to form the eupyrene sperm, there are a large number of tubulins (α-tubulin and βtubulin), which are not only the major component of centriole and spindle, but also play an important role in meiosis. It is concluded that the sHSPs may interact with the tubulin when the eupyrene sperm is forming and play an important role in the speeding of the axoneme formation and flagellar movement.

Key words: Bombyx mori , testis , small heat shock protein , eupyrene sperm , apolipophorin III

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