Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3264-3271.doi: 10.3864/j.issn.0578-1752.2011.15.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Study on the Coexistence of GnRH, OT, SYN and S-100 Protein in the Same Luteal Cell of the Mid Phases of Oestrous Cycle in Karakul Sheep

ZHENG  Mao-Liang, LI  Guang   

  1. 1. 塔里木大学动物科学学院
    2. 西北农林科技大学动物科技学院
  • Received:2010-10-20 Revised:2011-04-14 Online:2011-08-01 Published:2011-05-13

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Abstract: 【Objective】The experiment was conducted to study coexistence of gonadotropin-releasing hormone (GnRH), oxytocin (OT), synaptophysin (SYN) and S-100 protein of dispersed or diffuse neuroendocrine system (dispersed or diffuse neuroendocrine system，DNES) in the same luteal cell. 【Method】 The corpus luteum was stained using pairwise combinations of OT, GnRH, SYN, and S-100 monoclonal antibody through double labeling immunofluorescence staining, respectively, at the mid phases of oestrous cycle of the Karakul sheep. 【Result】 Five kinds of immunofluorescence staining double positive cells combinations (OT and GnRH, OT and S-100, OT and SYN, GnRH and S-100, and GnRH and SYN) were respectively presented in luteal cell. In addition, the correlation analysis on 10 combinations of 5 kinds of double positive cells showed that there were 9 highly correlated combinations (r＞0.7). 【Conclusion】 This result reveals that there are DNES cells widespread in the same luteal cells，and there are OT and GnRH widespread in DNES cells. So, DNES cells may be the secretion cells of OT, and GnRH, and there are dual properties of both DNES cells and endocrine cells in luteal cells.

Key words: GnRH, OT, SYN, S-100, luteal cell, Coexistence

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