Abstract: 【Objective】 The SNPs of IL-1β gene and their correlations with some immune traits were tested. 【Method】 A pair of primers were designed as the sequence of IL-1β of red jungle fowls, and the SNPs of intron 1 and 2 of IL-1β gene in Rugao Chicken(RG), Wenchang Chicken(WC), and Anka Chicken(AK) were detected using PCR-SSCP. Meanwhile, concentration of IL-1, H/L value, SRBC antibody titer, ND antibody titer and AI antibody titer were detected at 56 days of age. Then, the correlation between SNP sites and immune traits were analyzed. 【Results】 Totally 6 SNP sites were detected at T107G, T130A, C143T, A208C, T241G and T274C. Furthermore, the mutation at 107, 208, 241 and 274 sites were found association with immune traits (P＜0.05 or P＜0.01), and some genotype combinations significantly affected H/L value and ND antibody titer (P＜0.05). 【Conclusion】 The mutation at 241 site may be selected as a new candidate mark in disease resistance breeding because of its better performance in three immune traits than that of wild type chickens. Selecting breeder chickens by genotype combinations had more effect than by single genotype of SNPs.

Key words: IL-1&beta')">IL-1&beta, gene, SNP, immune traits, chicken