Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (3): 643-652.doi: 10.3864/j.issn.0578-1752.2021.03.017

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

The Influence of Low-Temperature and Long-Time Cooking on the Quality of Pork Products

WANG JingFan1(),HUANG Feng1,SHEN QingShan1,WEN YanTao2,GUO ZhiGang2,JING XiaoLiang2,ZHANG ChunHui1()   

  1. 1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193
    2Jiangsu Chaoyue Agricultural Development Co. LTD, Taixing 225400, Jiangsu
  • Received:2020-06-19 Accepted:2020-10-14 Online:2021-02-01 Published:2021-02-01
  • Contact: ChunHui ZHANG E-mail:wangjingfansnnu3@163.com;dr_zch@163.com

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Abstract:

【Objective】The objective of this study was to investigate the effect of low-temperature and long-time (LTLT) cooking on water holding capacity (WHC) and tenderness of pork. It was expected to illustrate the potential mechanism for the change of moisture distribution, muscle fiber structure, and collagenolysis of the LTLT cooked pork, so as to provide a scientific basis for the actual industrial application. 【Method】The pork Longissmus dorsi was used as experimental material which was boiled at different low-temperature (55℃ and 60℃) for long-time (4 h, 8 h, and 24 h) respectively. The quality characteristics of LTLT cooked pork, including WHC, color and tenderness, were analyzed by cooking loss rate, area shrinkage, low field nuclear magnetic resonance (LF-NMR), magnetic resonance imaging (MRI), color, and Warner-Bratzler shear force. The structural changes of myofibrils and connective tissue were observed by histological analysis. Then the surface hydrophobicity, collagen content, and its thermal solubility were analyzed.【Result】With the increasing of heating time and temperature, the influence of LTLT cooking on the quality of pork products and the protein denaturation increased. The muscle fiber structure was damaged during heating. Specifically, the cooking loss rate and area shrinkage ratio increased significantly (P<0.05); LF-NMR and MRI analysis demonstrated that the transverse relaxation time (T22) and peak area (A22) decreased markedly (P<0.05); the b* value increased (P<0.05). The shrinkage of myofibrils and collagen fibers in the perimysium dissolving occurred, the surface hydrophobicity enhanced significantly (P<0.05). The content of collagen decreased observably (P<0.05), while heat solubility increased. 【Conclusion】Compared with heating time, heating temperature had more significant effects on meat quality and protein denaturation, and these effects could be alleviated by extending the heating time. At different heating temperatures, the difference in the longitudinal shrinkage ratio was more significant, which might be caused by different denaturation of collagen, especially the insoluble collagen.

Key words: pork, low-temperature long-time cooking, myofiber, connective tissue, protein denaturation

Fig. 1

Effects of heating temperature and time on cooking loss Different capital letters indicate significantly differences among different heating temperatures and the same heating time (P<0.05); Different lowercase letters indicate significantly differences among same heating temperature and different heating time (P<0.05). The same as below"

Fig. 2

Effects of heating temperature and time on shear force"

Table 1

Effect of heating temperature and time on color"

55℃ 60℃
4 h 8 h 24 h 4 h 8 h 24 h
L* 81.28±0.73Aa 81.59±0.54Aa 81.61±0.50Aa 82.63±0.36Aa 82.79±0.51Aa 82.80±0.42Aa
a* 6.18±0.42Aa 6.57±0.37Aa 6.64±0.20Aa 5.50±0.21Aa 5.64±0.42Aa 5.77±0.26Ba
b* 6.00±0.30Ba 6.05±0.32Ba 6.14±0.16Ba 6.75±0.11Ab 6.79±0.06Ab 7.30±0.17Aa
?E 27.56±1.80Aa 27.87±1.26Aa 27.88±1.22Aa 28.97±0.90Aa 29.14±1.26Aa 29.19±1.05Aa

Fig. 3

Effect of heating temperature and time on area shrinkage"

Table 2

Moisture distribution and composition of pork after different temperature and time heating"

T21 (ms) T22 (ms) T23 (ms) A21 (%) A22 (%) A23 (%)
55℃-4h 0.56±0.18Aa 36.59±1.90Ab 207.87±17.17Aa 2.66±0.01Bb 96.08±0.00Ba 0.97±0.00Aa
55℃-8h 0.57±0.15Aa 34.91±1.33Aab 217.70±17.34 Ba 3.00±0.00Bab 95.89±0.00Aa 1.11±0.00Aa
55℃-24h 0.58±0.12Aa 33.73±1.48Aa 222.98±20.32 Ba 3.22±0.00Ba 95.85±0.00Aa 0.93±0.00Aa
60℃-4h 0.40±0.02 Aa 32.22±1.80Ba 215.61±23.17Ac 3.46±0.00Ab 95.69±0.00Aa 0.86±0.00Aa
60℃-8h 0.43±0.01 Aa 29.38±1.82Bb 250.13±19.93Ab 3.86±0.00Aa 95.32±0.00Ba 0.77±0.00Ba
60℃-24h 0.37±0.05Ba 29.36±1.29Bb 280.90±24.19Aa 3.91±0.00Aa 95.55±0.00Aa 0.50±0.00Bb

Fig. 4

Proton density weighted pseudo color images of pork after different temperature and time heating"

Fig. 5

Sections of muscle tissue in meat during different temperature and time heating"

Fig. 6

Effect of heating temperature and time on protein surface hydrophobicity"

Table 3

Effect of heating temperature and time on collagen"

指标
Index		 55℃ 60℃
4 h 8 h 24 h 4 h 8 h 24 h
总胶原蛋白含量
Total collagen content (mg?g-1)		 1.34±0.09Aa 0.74±0.05Ab 0.61±0.03Ab 0.80±0.05Ba 0.55±0.02Bb 0.49±0.06Ab
热溶性胶原蛋白含量
Heat soluble collagen content (mg?g-1)		 0.10±0.07Ab 0.12±0.01Aab 0.13±0.00Aa 0.10±0.05Bb 0.14±0.00Aa 0.13±0.00Aa
胶原蛋白溶解度 Collagen solubility 0.09±0.01Bb 0.19±0.01Aa 0.17±0.01Aa 0.17±0.00Ab 0.17±0.02Ab 0.29±0.04Aa
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