Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4698-4706.doi: 10.3864/j.issn.0578-1752.2013.22.007

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Biofumigation with Rapeseed Meal on Disease Control of Phytophthora Blight of Chilli Pepper

 MA  Yan, HU  An-Yi, YANG  Hao, CHANG  Zhi-Zhou, XU  Yue-Ding, ZHANG  Jian-Ying   

  1. Institute of Agricultural Resources and Environments, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2013-05-02 Online:2013-11-15 Published:2013-07-09

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Abstract: 【Objective】The objective of this study is to research the effects of hydrolysis product of rapeseed meal (RSM) on growth inhibition of Phytophthora capsici and on disease control of Phytophthora blight of chilli pepper cultivation in greenhouse. 【Method】 Inhibition effect of hydrolysis product of RSM on mycelium and zoospore was studied by in vitro test in dishes and imitated biofumigation in container, and disease control effects of biofumigation with RSM2 on Phytophthora blight of chilli pepper in pot and field were also carried out. 【Result】 Volatiles and non-volatiles released from RSM hydrolysis inhibited mycelium growth and zoospore germination in various degrees, and the inhibition effect of RSM2 was better than that of RSM1. The inhibition effect of volatiles produced from RSM hydrolysis on zoospore was stronger than on mycelium of P. capsici while non-volatiles displayed stronger inhibition effect on mycelium than on zoospore. The inhibition effect of volatiles and non-volatiles showed no significant difference on mycelium but on zoospore of P. capsici. Soil fumigation in sealed container was carried out in laboratory at different application rates of RSM and the results showed that volatiles released from RSM hydrolysis killed mycelium of P. capsici when RSM was added to soil at the rate of 0.2% (w/w). In a pot experiment, real-time PCR was adopted to assess the population of P. capsici. The results showed that the efficacy of disease control of RSM treatment for Phytophthora blight was 100%, the same as dazomet treatment, although P. capsici exsisted in soil at density of 36 zoospore/g soil. RSM2 treatment not only increased the population of fungi and actinomycetes but also increased the total number and diversity of soil microbe. The plot experiment conducted in greenhouse showed that the average value of control efficacy for Phytophthora blight was 82% and the yield of chilli pepper increased with 16.4% in two continuous cropping seasons. The effect of RSM treatment in chilli pepper production in protected field was better than dazomet treatment. 【Conclusion】RSM2 showed great inhibition effect on growth of P. capsici. The treatment of biofumigation with RSM2 suppressed disease incidence of Phytophthora blight in field efficiently and increased yield of chilli pepper.

Key words: biofumigation , rapeseed meal , Phytophthora capsici , soil microbe , Phytophthora blight of chilli pepper

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