光敏蛋白脂质体的制备与生物活性

doi:10.3864/j.issn.0578-1752.2013.01.008

Abstract

Abstract: 【Objective】The objective of this study is to research the preparation prescription of KillerRed protein liposome (L-KRP) and its physicochemical properties and bioactivity for optimizing the bioefficacy of KRP. 【Method】 L-KRP was prepared by reverse-phase evaporation method, encapsulation efficiency of L-KRP was determined by the fluorescence spectrophotometry, orthogonal experiment was adopted to optimize the prescription using encapsulation efficiency as the evaluation parameter. The appearance, size, Zeta potential, leakage rate and bioactivity of L-KRP were investigated. 【Result】 The dispersed liposome was spherical with the mean diameter of 520.9 nm, the Zeta potential of -83.78 mV and the mean encapsulation efficiency of 77.05%. The toxicity regression equations of L-KRP against the 4th instars of Aedes albopictus were y=3.0499x+0.0368 (r=0.9771), y=2.6003x+1.1592 (r=0.9600) and y=2.7702x+1.2624 (r=0.9765) at different times, with their LC50 were 42.40, 30.00 and 22.35 μg•mL-1, respectively. The permeability of L-KRP was better than KRP through the epidermis of Bombyx mori and Spodoptera litura, the accumulated permeation through B. mori and S. litura were 24.67 and 20.83 μg•mm-2 after 48 hours, with 3.10 and 2.35 times of KRP, respectively.【Conclusion】 All the bioactivity and permeability of L-KRP were higher than KRP.

Key words: KillerRed protein , liposome , bioactivity , transdermal permeability

YIN Su-Xia, LIAO Mei-De, XU Han-Hong. Preparation of Photosensitive Protein Liposome and Its Bioactivity[J].Scientia Agricultura Sinica, 2013, 46(1): 60-68.

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