鹿慢性消耗性疾病免疫组织化学检测方法的建立与优化

doi:10.3864/j.issn.0578-1752.2012.15.020

Abstract

Abstract: 【Objective】The purpose of this study is to establish and optimize immunohistochemical assay for detection of chronic wasting disease (CWD) based on the monoclonal antibodies (McAbs) against Elk cellular prion protein which were independently developed by China Animal Health and Epidemiology Center. 【Method】Negative and positive slices were made by conventional paraffin method,and ABC method of IHC was used to detect the 5 McAbs.【Result】The results indicated that only two (No. 5E3 and 3B2) from 5 McAbs (No. 5A5, 3B2, 6D12, 5E3 and 1F5, respectively ) could be used for the immunohistochemical detection of positive spinal cord section of Elk. It was showed strong specific immunoreactivity with cervid abnormal prion protein by 5E3 and weak specific immunoreactivity by 3B2. There was no any immunoreactivity by other McAbs with various experimental concentrations. Additionally, the best immunoreactivity was present when McAb 5E3 was used at 1/10000 dilution. 【Conclusion】The study demonstrated that 5E3 can be used in national surveillance of CWD.

Key words: elk, chronic wasting disease, monoclonal antibodies, immunohistochemistry

ZHANG Xin-Xin, LIU Huan-Qi, LIU Yu-Tian, SUN Cheng-You, CHI Tian-Ying, YU Xiao-Jing, DENG Ming-Yi, WANG Zhi-Liang. Establishment and Optimization of Immunohistochemistry Assay for Detection of Chronic Wasting Disease[J].Scientia Agricultura Sinica, 2012, 45(15): 3176-3181.

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Establishment and Optimization of Immunohistochemistry Assay for Detection of Chronic Wasting Disease

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