Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2999-3006.doi: 10.3864/j.issn.0578-1752.2012.14.024

• VETERINARY SCIENCE • Previous Articles    

Histological Observation and Expression Patterns of GABA in Development of Ostrich Chicks’ Paraventricular Hypothalamic Nucleus

 TANG  Li, WEI  Lan, ZHANG  Yong, PENG  Ke-Mei   

  1. 1.四川农业大学动物医学院，四川雅安 625014
    2.华中农业大学动物医学院，武汉 430070
    3.河南科技大学动物科技学院，河南洛阳 471003
    4.西北农林科技大学动物医学院，陕西杨凌 712100
  • Received:2011-11-15 Online:2012-07-15 Published:2012-02-27

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Abstract: 【Objective】The experiment was conducted to study the development characteristics of the microstructure and apoptosis in paraventricular hypothalamic nucleus (PVN) of ostrich chicks. The Age-related change of γ-aminobutyric acid (GABA) positive cells was also investigated. 【Method】One, 45 and 90 d healthy ostrich chicks were selected from a normal group. The development characteristics of PVN in ostrich chicks was detailly observed by HE staining, terminal deoxynucleotidyl transferase-mediated (TUNEL) and immunohistochemistry (SABC). 【Result】 The results showed that the histological structure of PVN developed maturity gradually with the ages of ostrich chicks. The cell proportion of parvocellular PVN was the on lowest on 45 d than that on 1 d and 90 d. A less number and an age-related decline of apoptosis in PVN could be found. The positive signals of GABA positive cells could be detected in PVN, with the expression increased with age growing of ostrich chicks, and the average optical density of magnocellular paraventricular nucleus (mPVN) and parvocellular paraventricular nucleus (pPVN) reached the maximum on 45 d (P＜0.01).【Conclusion】These results demonstrate that the structure and function of PVN in ostrich chicks is under continuous perfection and development, especially in early postnatal period (1-45 d).

Key words: ostrich chicks, paraventricular hypothalamic nucleus, γ-aminobutyricacid, histology, apoptosis

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