Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1857-1863.doi: 10.3864/j.issn.0578-1752.2012.09.022

• RESEARCH NOTES • Previous Articles     Next Articles

Analysis on Molecular Evolution of HbSUT3 in Hevea Species

 LIN  Fei-Peng, FAN  Yu-Jie, LONG  Xiang-Yu, HU  Yan-Shi, TANG  Chao-Rong   

  1. 1.海南大学农学院，海南儋州 571737
    2.中国热带农业科学院橡胶研究所，海南儋州 571737
  • Received:2011-12-16 Online:2012-05-01 Published:2012-02-16

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Abstract: 【Objective】The purpose of this work was to investigate the molecular evolution of HbSUT3 and its correlation with latex yield. 【Method】In this study, 21 cDNAs and 10 genomic DNAs of HbSUT3 and its homologs were cloned from 21 Hevea samples, including five large-scale recommended H. brasiliensis varieties, nine H. brasiliensis 1981’ IRRDB germplasms, and seven other Hevea species. Molecular evolution analysis of these sequences was made mainly by using MEGA software, and the phylogenetic trees were constructed by the neighbor-joining algorithm.【Result】The results showed that the genomic clones of all HbSUT3 and its homologs contained four exons and three introns, and all had a cDNA coding sequence of 1 608 bp. The ratios of transitions to transversions between different samples were lower than 2.0, and the values of Ks were generally larger that those of Ka for maximum. Phylogenetic tree showed that high-yielding H. brasiliensis samples tended to cluster together, but samples of several Hevea species could also be grouped together. 【Conclusion】 HbSUT3 and its homologs are evolutionally conservative, and the molecular evolution of HbSUT3 has a correlation with latex yield. In addition, the evolutionary analyses provide molecular evidence for the idea that the genus Hevea can be considered as species complex.

Key words: Hevea, HbSUT3, molecular evolution, latex yield

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