Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (3): 548-555.doi: 10.3864/j.issn.0578-1752.2012.03.017

• VETERINARY SCIENCE • Previous Articles     Next Articles

Establishment of a Bird Experiment Model for Chicken Acute Fibrosarcomas Induced by ALV-J Associated Virus

 LI  Chuan-Long, ZHANG  Heng, ZHAO  Peng, CUI  Zhi-Zhong   

  1. 1.山东农业大学动物科技学院,山东泰安 271018
  • Received:2011-05-19 Online:2012-02-01 Published:2011-11-29

Abstract: 【Objective】 An experiment was carried out to prove that the field chicken fibrosarcoma mass contains acute fibrosarcoma-inducing ALV-J associated virus. 【Method】 One-day-old 817 hybrid chickens were inoculated through different routes with tumor suspension filtrate containing ALV-J associated virus. One-day-old 817 hybrid chickens and SPF Leghan chickens were inoculated subcutaneously with tumor suspension filtrate containing ALV-J associated virus in different doses. Continuous observation of tumor development was made in 30 days. 【Result】 In the groups of inoculations through the subcutaneous, in muscule and intra-abdominal, the tumor incidence was 100% (20/20), 95.2% (20/21), and 100% (20/20), respectively. The tumor incidence of 817 hybrids and SPF chickens inoculated with undiluted tumor extract filtrate was 100% (10/10). When birds were inoculated with tumor extracts in 1﹕10 and 1﹕100, tumor rates were still in the range of 80% (8/10) to 100% (10/10) for both chicken lines. When birds were inoculated with tumor extracts in 1﹕1 000, tumor rates decreased to 40% (4/10) or 50% (5/10). No tumor development was observed in 30 days when birds were inoculated with the tumor extract of 1﹕10 000 dilution. However, there were 2/6 chickens developed tumor mass when inoculated with supernatant from tumor extract-infected DF-1 cell culture. Histopathology indicated that the induced tumors were typical fibrosarcoma.【Conclusion】The fibrosarcoma tissues of 817 hybrid chickens of field case contained ALV-J associated acutely tumorigenic virus. Both tumor extract filtrate or extract-infected DF-1 cell culture supernatant could induce acute fibrosarcomas in chickens of different lines, and the tumor started to appear as early as 7 days after inoculation. The dynamics of the fibrosarcoma development and tumor rates were closely related to doses of the tumor extracts in inoculation.

Key words: acute chicken fibrosarcomas, subgroup J avian leukosis virus, bird experiment model

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