Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3446-3453.doi: 10.3864/j.issn.0578-1752.2011.16.018

• VETERINARY SCIENCE • Previous Articles     Next Articles

The Function of PI3K/Akt Signal Pathway During ALV-J Infection in DF-1 Cells

FENG  Shao-Zhen, LI  Jiao, WU  Xiao-Chan, CAO  Wei-Sheng, LIAO  Ming   

  1. 华南农业大学兽医学院/农业部动物疫病防控重点开放实验室
  • Received:2011-04-14 Revised:2011-05-11 Online:2011-08-15 Published:2011-06-22
  • Contact: Shao-zhen FENG E-mail:fengshaozhen111@hotmail.com

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Abstract: 【Objective】 Given the biological importance of the PI3K/Akt pathway in virus infection, this experiment investigated whether or not the subgroup J avian leukosis virus (ALV-J) strain HN06 infection in DF-1 cells is correlated with the activity of Akt. 【Method】After inoculation of ALV-J strain HN06 which mainly induces hemangiomas and strain NX0101 which induces myelocytomas in DF-1 cells, respectively, the expression levels of Akt phosphration, viral RNA transcription and viral protein synthesis were analyzed.【Result】 Results showed that the replication rates in vivo of ALV-J strains HN06 and NX0101 were different. HN06 infection led to increased Akt phosphorylation at a very early stage which was virus titer-dependent. LY294002, a PI3K-specific inhibitor, could suppress HN06 induced Akt phosphorylation, indicating that Akt phosphorylation was PI3K-dependent. Moreover, in the presence of LY294002, viral RNA transcription level, viral envelope protein ENV expression and virons secreted in supernatants were suppressed significantly. The PI3K/Akt activation profile and function of HN06 were similar to that of NX0101 reported. 【Conclusion】These data suggest that PI3K/Akt signaling pathway plays an important role in ex vivo ALV-J replication, although the precise mechanism remains under investigation.

Key words: Subgroup J avian leukosis virus, Hemangioma, myelocytomas, Virus early infection, PI3K/Akt signal pathway

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  • A

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