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Analysis of a hAT Family Transposase Gene in Bombyx mori

SHEN Xiao-juan1, SUN Xia1, WANG Sheng-peng2, GUO Xi-jie2   

  1. 1、College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu;
    2、The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu
  • Received:2010-07-05 Online:2011-04-02 Published:2010-09-30

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Abstract: 【Objective】The objective of this study is to analyze a latent hAT transposase gene of Bombyx mori, named as BmhAT. 【Method】Sequence homology and structural domain of BmhAT were studied using bioinformatical method, the copy number of BmhAT in Bombyx mandarin and different varieties of Bombyx mori were measured by Real-time PCR, and the relative expression of BmhAT at mRNA level in different tissues of Bombyx mori was analyzed by Real-time RT-PCR.【Result】Bioinformatical analysis revealed that BmhAT had significant homology to some hAT family transposase in sequence and contains conserved domains of this family transposase. Real-time PCR results showed that BmhAT had about 3-6 copies in Bombyx mori genome. Quantitative real-time RT-PCR results indicated that the relative expression of BmhAT to actin3 reference gene in posterior silk gland and fat body was 0.4 folds and 0.6 folds, respectively.【Conclusion】BmhAT may be an active transposase gene in Bombyx mori, encoding a new hAT family transposase of Bombyx mori.

Key words: Bombyx mori , hAT family transposon , transposase

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