Abstract:

【Objective】 Fermentation technology was explored to increase the yield of coronatine which was producted by a high-producing mutant strain Pseudomonas syringae pv. glycinea M-18. 【Method】 The effect of initial glucose concentration, feeding concentration of glucose, feeding time, feeding times and pH were investigated in shaker firstly and then in 5 L stirred bioreactors. 【Result】 The experiment results showed that with an initial glucose concentration at 10 g•L-1, feeding 0.005 mmol FeCl3 per litre fermentation broth after 84 h, keeping glucose concentration between 5-2 g•L-1 and buffer pH at 6.7 with 40 g•L-1 MgCO3, the production of coronatine increased by 31% in the fed batch fermentation compared with batch fermentation.【Conclusion】 The production of coronatine produced by P. syringae pv. glycinea M-18 can be increased evidently when the concentration of glucose was controlled in a appropriate range.

Key words: coronatine')">coronatine, fed-bach fermentation, Pseudomonas syringae, pH