Abstract:

【Objective】 The physiological responses of grape roots under CdCl2 treatment were investigated for exploring the difference of endurance to CdCl2 stress among cultivars of grape. 【Method】 The experiment was carried out with the cutting seedlings of nine grape cultivars under 0.5 mmol•L-1CdCl2 stress via hydroponics. The changes in the activity of root, the activity of H+-ATPase in plasma membrane (PM) and the malondialdehyde (MDA) content of roots after CdCl2 treatment were studied. 【Result】 The results showed that the root activities of the nine cultivars under normal circumstances was in the order as below: Muscat Hamburg＞Kyoho＞Guibao＞Autumn Royal＞Qtilia Seedless＞Zana＞Red Globe＞Zexiang＞Tamina. After being treated in 0.5 mmol·L-1 CdCl2 for 16 hours, the root activity of all the cultivars decreased and the decrease range according to the order from high to low as follow: Kyoho＞Autumn Royal＞Muscat Hamburg＞Guibao＞Red Globe＞Zana＞Tamina＞Zexiang＞Qtilia Seedless. The activity of PM H+-ATPase in root of all the cultivars decreased and the root MDA content increased after treatment of CdCl2. Among the nine grape cultivars, Kyoho presented the greatest decrease in the activity of PM H+-ATPase and the greatest increase in its MDA content, while Qtilia Seedless presented the greatest increase in its MDA content. 【Conclusion】 The sensitivity and endurance of grape roots to the CdCl2 stress were different significantly among different cultivars. The root activity and PM H+-ATPase activity of Kyoho was the most sensitive to cadmium and Kyoho was the most affected by cadmium stress among the nine cultivars, while the root activity of Qtilia Seedless has better endurance to the CdCl2 stress than that of other cultivars and was the least sensitive to CdCl2 treatment.

Key words: grape')">grape, CdCl2, root activity, cultivar