Abstract:

【Objective】 Glycosyl hydrolase family 62 encode putative alpha-L-arabinofuranosidases, a group of cell-wall degrading enzymes which may involve in the fungal pathogenesis. To better understand the role of cell-wall degrading enzymes in pathogenesis, the putative glycosyl hydrolase family 62 in Magnaporthe grisea we characterized in this study. 【Method】 The bioinformatics tools were firstly used to analyze putative members in the annotated glycosyl hydrolase family 62, including their gene structure, protein secretion characters, and conduct a phylogenetic analysis. Then overexpression and knockout techniques were applied to further study the function of MGG_01403.6, one of the 8 members in the family. 【Result】 The result showed that all the members are secreted proteins with cell-wall degrading enzyme (Glycosyl hydrolase family 62) domain. Phylogenetic analysis showed that 8 members of glycosyl hydrolase family 62 of the fungus could be classified into 2 clusters. Gene expression showed difference among family members after fungal infection. But gene knockout and overexpression of MGG_01403.6 had no effect on the fungal pathogenicity. 【Conclusion】 Functional redundancy may exist in glycosyl hydrolase family 62 members from Magnaporthe grisea. The double or multiple deletions are necessary for further characterization of the function of this multi-gene family.

Key words: Magnaporthe grisea, cell-wall degrading enzymes, glycosyl hydrolase family 62