Abstract:

【Objective】 In this study, the objective is to screen and clone the drought stress resistance-related genes, and analyze the expression pattern of those genes which aim to probe into the molecular mechanism of drought resistance and provide candidate genes for drought-resistance plant breeding. 【Method】 Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs, a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends (RACE) method, and the gene expression character was analyzed by real time RT-PCR. 【Result】 Four obvious up-regulated ESTs of SSADH were obtained in the drought stress Erianthus arundinaceus througth cDNA microarray hybridization. The full-length cDNA of SSADH termed as sc-SSADH from sugarcane was 1 914 bp in length, contains a 1 581 bp open reading frame (ORF) encoding a 527 amino acid protein, with 25 bp in the 5′ UTR and 306 bp in the 3′ UTR, including a poly (A) signal at 1 749 position. GenBank Blast analysis showed that SSADH was 73% identical to Arabidopsis thaliana SSADH (NM_106592.3). Real time RT-PCR analysis revealed that the sc-SSADH was a whole range PEG-stress responsive gene, and it could also be regulated by Ca2+ in sugarcane. 【Conclusion】 It is the first report of the cloning and expression analysis of SSADH in sugarcane, and the sc-SSADH is a dehydration-responsive gene. At the same time, Ca2+ has been proposed to play a role in the regulation of SSADH

Key words: Saccharum officinarum L., water stress, ALDH, SSADH