中国农业科学 ›› 2007, Vol. 40 ›› Issue (1): 41-47 .

• 作物遗传育种·种质资源 • 上一篇    下一篇

大豆主要农艺性状的QTL分析

陈庆山,张忠臣,刘春燕,辛大伟,单大鹏,邱红梅,单彩云   

  1. 东北农业大学大豆研究所
  • 收稿日期:2005-11-07 修回日期:1900-01-01 出版日期:2007-01-10 发布日期:2007-01-10
  • 通讯作者: 陈庆山

QTL Analysis of Main Agronomic Traits in Soybean

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  1. 东北农业大学大豆研究所
  • Received:2005-11-07 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10

摘要: 【目的】大豆的多数农艺性状均为重要的数量性状。对大豆的数量性状进行基因定具有重要的研究和应用价值。【方法】以美国半矮秆大豆品种Charleston为母本,东北农业大学高蛋白大豆品系东农594为父本及其F2:10代重组自交系的154个株系为实验材料。164个SSR引物经亲本筛选后用于群体扩增,并构建遗传图谱。对亲本间表现多态的12个农艺性状进行了调查及QTL分析。【结果】农艺性状包括品质性状(蛋白质含量、油分含量、蛋脂总量等);产量性状(单株荚数、单株粒重、百粒重等)和其它农艺性状(株高、生育期、分枝数、主茎节数、平均叶长、平均叶宽等)。结果表明,12个农艺性状共检出68个QTLs。每个性状的QTLs检出个数从平均叶宽的3个到百粒重、株高等的8个,平均每个性状检测出5.8个。与国内外对应农艺性状QTL检测结果相比,多个性状的QTL位点均一致,说明QTL检测准确率较高,可以进一步用于分子辅助育种。【结论】获得了大豆12个重要农艺性状的68个主效QTLs。

关键词: 大豆, 农艺性状, QTL, SSR, 分析

Abstract: F2:10 RIL population with 154 lines, crossed by Charleston as female parent and Dongnong 594 as male parent were used. 164 SSR primers were screened with the two parents and amplified on the 154 lines. 12 agronomic traits different between the two parents are investigated, and QTLs of all traits are analyzed with windows QTL Cartographer V2.0. The agronomic traits includes quality traits: content of protein, content of oil, total content of protein and oil; yield traits: pods per plant, seed weight per plant, and seed weight per hundred; and other traits: plant height, maturity period, branches, main stem nodes, average leaf length, average leaf width. The results show that: 68 QTLs are scanned out for the 12 agronomic traits. QTLs of each trait varied from 3 of average leaf width to 11 of seed weight per hundred and plant height, and the average is 5.8. Compared with the QTLs results at home and abroad, more QTL loci are identical, so they can be used for MAS.

Key words: Soybean, Agonomic trait, QTL, SSR, Analysis