





中国农业科学 ›› 2019, Vol. 52 ›› Issue (3): 428-444.doi: 10.3864/j.issn.0578-1752.2019.03.004
收稿日期:2018-07-05
接受日期:2018-12-28
出版日期:2019-02-01
发布日期:2019-02-14
联系方式:
魏廷邦,E-mail: weitingbang@163.com。
基金资助:
WEI TingBang1,CHAI Qiang2(
),WANG WeiMin1,WANG JunQiang1
Received:2018-07-05
Accepted:2018-12-28
Published:2019-02-01
Online:2019-02-14
摘要:
目的 针对土壤水分、氮肥供应不足以及玉米早衰、种植密度不合理等严重制约绿洲灌区玉米的生产问题,通过研究不同水氮配比及种植密度对玉米光合作用、干物质积累特征和产量的影响,以期为该区玉米高产、稳产提供技术支撑。方法 2016—2017年,于河西绿洲灌区进行大田试验,以先玉335为参试品种,采用裂裂区设计,灌水水平(W1:4 050 m 3·hm -2,W2:3 720 m 3·hm -2)做主区,施氮水平(不施氮N0:0,低施氮N1:300 kg·hm -2,高施氮N2:450 kg·hm -2)为裂区,种植密度(低密度D1:75 000株/hm 2,中密度D2:97 500株/hm 2,高密度D3:120 000 株/hm 2)为裂裂区,测定光合速率、干物质积累量和产量等指标。 结果 施氮量、种植密度对玉米全生育期净光合速率、干物质最大增长速率及其出现天数、干物质积累量、产量、WUE和氮肥利用率有显著影响。水肥耦合可增强玉米密植条件下的光合作用,提高干物质最大增长速率,提前干物质最大增长速率出现的天数,增大干物质积累量和产量。在减量20%灌水和高施氮水平下,中密度处理的全生育期净光合速率较低密度和高密度分别提高17.31%和11.43%;高密度和中密度处理的干物质最大增长速率及最大增长速率出现天数较低密度处理分别提高21.07%、7.52%和提前6.7 d、4.1 d;高密度处理的干物质积累量较中密度、低密度分别提高4.27%和10.59%,中密度处理的产量、水分利用效率和氮肥利用率较低密度、高密度处理分别提高24.2%、11.4%、29.9%和29.2%、18.4%、13.8%。在减量20%灌水条件下,中密度高施氮处理的全生育期净光合速率、干物质积累量和产量分别较中施氮、不施氮分别提高7.34%、11.63%、14.63%和49.54%、44.53%、69.03%;高密度高施氮处理的干物质最大增长速率及最大增长速率出现天数较中施氮、不施氮分别提高19.07%、54.35%和提前3.9 d、6.8 d;同等密度高施氮处理的氮肥利用率较低施氮处理提高24.5%。综上,减量20%灌水与高施氮耦合主要通过提高密植玉米的光合作用和干物质积累速率,延长干物质积累的持续时间,提高WUE和氮肥利用率,从而对干物质积累量和产量产生调控作用。结论 在绿洲灌区,采用水肥耦合(生育期减量20%灌水(3 720 m 3·hm -2)、施氮量450 kg·hm -2、中密度97 500株/hm 2)的最优栽培模式,可为进一步发掘密植条件下玉米高产、高效栽培提供技术指导。
魏廷邦, 柴强, 王伟民, 王军强. 水氮耦合及种植密度对绿洲灌区玉米光合作用和 干物质积累特征的调控效应[J]. 中国农业科学, 2019, 52(3): 428-444.
WEI TingBang, CHAI Qiang, WANG WeiMin, WANG JunQiang. Effects of Coupling of Irrigation and Nitrogen Application as well as Planting Density on Photosynthesis and Dry Matter Accumulation Characteristics of Maize in Oasis Irrigated Areas[J]. Scientia Agricultura Sinica, 2019, 52(3): 428-444.
表1
不同处理玉米干物质积累阶段特征"
| 年份 Year | 处理 Treatment | 渐增期Early stage | 快增期Fast stage | 缓增期Late stage | |||
|---|---|---|---|---|---|---|---|
| 持续时间 Duration (d) | 平均积累速率 Average accumulate rate (kg·hm-2·d-1) | 持续时间 Duration (d) | 平均积累速率 Average accumulate rate (kg·hm-2·d-1) | 持续时间 Duration (d) | 平均积累速率 Average accumulate rate (kg·hm-2·d-1) | ||
| 2016 | W1N0D1 | 66.50b | 76.13c | 32.12b | 430.64q | 46.37o | 85.34o |
| W1N0D2 | 64.77c | 88.41bc | 33.76b | 463.38p | 46.45n | 116.33c | |
| W1N0D3 | 62.79d | 92.21b | 30.62b | 516.46l | 51.58f | 95.32j | |
| W1N1D1 | 65.81bc | 100.75ab | 35.12b | 515.82m | 44.06q | 100.53g | |
| W1N1D2 | 65.45bc | 104.36a | 32.12b | 636.67f | 47.43m | 108.41e | |
| W1N1D3 | 61.26de | 111.59a | 32.52b | 677.32d | 51.22g | 121.62b | |
| W1N2D1 | 64.07cd | 102.04ab | 30.98b | 576.42i | 49.94k | 70.29r | |
| W1N2D2 | 61.33de | 109.84a | 28.32b | 649.74e | 55.35c | 86.39m | |
| W1N2D3 | 60.35e | 112.02a | 24.16b | 742.14b | 60.48a | 88.13l | |
| W2N0D1 | 68.69a | 76.62c | 37.63b | 382.14r | 38.68r | 70.88q | |
| W2N0D2 | 68.79a | 78.78c | 30.98b | 477.79o | 45.22p | 86.16n | |
| W2N0D3 | 66.24bc | 86.11bc | 30.98b | 502.83n | 47.77l | 100.07h | |
| W2N1D1 | 64.33cd | 91.13b | 29.93b | 535.09k | 50.74i | 81.52p | |
| W2N1D2 | 62.26de | 107.24a | 32.52b | 560.97j | 50.22j | 110.54d | |
| W2N1D3 | 61.19d | 110.04a | 32.12b | 624.83h | 51.68e | 130.56a | |
| W2N2D1 | 65.97bc | 102.18ab | 28.94b | 636.24g | 50.08h | 88.83k | |
| W2N2D2 | 62.71d | 112.37a | 28.32b | 679.77c | 53.96d | 99.16i | |
| W2N2D3 | 61.44de | 118.15ab | 25.82a | 768.09a | 57.74b | 105.34f | |
| 2017 | W1N0D1 | 66.85a | 97.26n | 30.27b | 586.77m | 27.87o | 29.41h |
| W1N0D2 | 82.73a | 87.73o | 37.09ab | 534.56p | 25.17q | 42.23c | |
| W1N0D3 | 73.48a | 114.04h | 41.15a | 556.33n | 30.36k | 45.06b | |
| W1N1D1 | 76.95a | 107.41i | 33.46ab | 674.74i | 34.58i | 34.72f | |
| W1N1D2 | 81.21a | 106.13k | 35.12ab | 670.54j | 28.66n | 58.69a | |
| W1N1D3 | 78.71a | 115.14g | 35.59ab | 695.58h | 30.71j | 40.03d | |
| W1N2D1 | 73.42a | 121.35e | 34.52ab | 705.13e | 37.05g | 21.12l | |
| W1N2D2 | 77.34a | 133.04c | 40.21a | 699.08g | 27.44p | 5.75o | |
| W1N2D3 | 75.55a | 139.23a | 39.31ab | 731.05d | 40.13d | 13.03n | |
| W2N0D1 | 74.97a | 75.99q | 35.59ab | 437.31r | 34.43i | 27.87i | |
| W2N0D2 | 76.51a | 85.37p | 33.64ab | 530.42q | 34.86h | 33.83g | |
| W2N0D3 | 71.01a | 102.82l | 36.58ab | 545.18o | 37.42f | 23.93k | |
| W2N1D1 | 80.54a | 97.67m | 35.21b | 610.42l | 29.24l | 19.41m | |
| W2N1D2 | 79.74a | 106.59j | 36.08ab | 643.61k | 29.18m | 28.27i | |
| W2N1D3 | 71.57a | 123.82d | 33.01ab | 733.65c | 40.42c | 41.92c | |
| W2N2D1 | 71.51a | 120.96f | 33.77ab | 699.91f | 39.72e | 25.64j | |
| W2N2D2 | 67.97a | 139.22a | 34.16ab | 756.88b | 42.86b | 38.81e | |
| W2N2D3 | 71.68b | 134.41b | 30.98b | 849.51a | 45.33a | 44.42b | |
表2
不同处理玉米干物质积累速率的Logistic 方程回归分析"
| 年份 Year | 处理 Treatment | 回归方程 Regression equation | R2 | 最大增长速率 出现天数 The days of MIR (d) | 最大增长速率 Maximum increase rate (kg·d-1·hm-2) |
|---|---|---|---|---|---|
| 2016 | W1N0D1 | Y = 23959.28 / (1 + e6.77 - 0.082 t) | 0.993 | 82.56c | 491.16b |
| W1N0D2 | Y = 27102.43 / (1 + e6.370 - 0.078 t) | 0.998 | 81.66c | 528.49b | |
| W1N0D3 | Y = 27397.08 / (1 + e6.717 - 0.086 t) | 0.994 | 78.10gh | 589.03ab | |
| W1N1D1 | Y = 31376.54 / (1 + e6.253 - 0.075 t) | 0.996 | 83.37c | 588.31ab | |
| W1N1D2 | Y = 35421.91 / (1 + e6.684 - 0.082 t) | 0.991 | 81.51d | 726.14ab | |
| W1N1D3 | Y = 38148.18 / (1 + e6.279 - 0.081 t) | 0.995 | 77.51h | 772.50ab | |
| W1N2D1 | Y = 30937.64 / (1 + e6.763 - 0.085 t) | 0.989 | 79.56f | 657.42ab | |
| W1N2D2 | Y = 31873.15 / (1 + e7.020 - 0.093 t) | 0.987 | 75.48i | 741.05a | |
| W1N2D3 | Y = 33991.32 / (1 + e7.895 - 0.109 t) | 0.996 | 78.43g | 926.26ab | |
| W2N0D1 | Y = 24905.35 / (1 + e6.125 - 0.07 t) | 0.998 | 87.50a | 435.84b | |
| W2N0D2 | Y = 25644.27 / (1 + e7.164 - 0.085 t) | 0.989 | 84.28b | 544.94ab | |
| W2N0D3 | Y = 26987.54 / (1 + e6.947 - 0.085 t) | 0.994 | 81.72d | 573.48ab | |
| W2N1D1 | Y = 27740.45 / (1 + e6.978 - 0.088 t) | 0.992 | 79.29f | 610.29ab | |
| W2N1D2 | Y = 31595.12 / (1 + e6.360 - 0.081 t) | 0.996 | 78.52g | 639.80ab | |
| W2N1D3 | Y = 34762.66 / (1 + e6.335 - 0.082 t) | 0.998 | 77.25h | 712.63ab | |
| W2N2D1 | Y = 31896.55 / (1 + e7.320 - 0.091 t) | 0.983 | 80.44e | 725.64ab | |
| W2N2D2 | Y = 33346.21 / (1 + e7.149 - 0.093 t) | 0.987 | 76.87h | 775.29a | |
| W2N2D3 | Y = 34354.051/ (1 + e7.584 - 0.102 t) | 0.991 | 74.35j | 876.02ab | |
| 2017 | W1N0D1 | Y = 30769.12 / (1 + e7.133 - 0.087 t) | 0.906 | 81.98r | 669.22m |
| W1N0D2 | Y = 34348.66 / (1 + e7.191 - 0.071 t) | 0.949 | 101.28a | 609.68p | |
| W1N0D3 | Y = 39656.88 / (1 + e6.020 - 0.064 t) | 0.967 | 94.06h | 634.51n | |
| W1N1D1 | Y = 39113.70 / (1 + e7.373 - 0.079 t) | 0.970 | 93.68i | 769.56i | |
| W1N1D2 | Y = 40787.85 / (1 + e7.408 - 0.075 t) | 0.968 | 98.77b | 764.77j | |
| W1N1D3 | Y = 42882.84 / (1 + e7.141 - 0.074 t) | 0.976 | 96.50f | 793.33h | |
| W1N2D1 | Y = 42160.80 / (1 + e6.919 - 0.076 t) | 0.959 | 90.68l | 804.22e | |
| W1N2D2 | Y = 45691.69 / (1 + e6.383 - 0.066 t) | 0.948 | 97.45e | 797.32g | |
| W1N2D3 | Y = 47778.52 / (1 + e6.379 - 0.067 t) | 0.954 | 95.21g | 833.79d | |
| W2N0D1 | Y = 26960.35 / (1 + e6.865 - 0.074 t) | 0.940 | 92.77k | 498.76r | |
| W2N0D2 | Y = 30904.47 / (1 + e7.307 - 0.078 t) | 0.971 | 93.32j | 604.95q | |
| W2N0D3 | Y = 34544.28 / (1 + e6.429 - 0.072 t) | 0.969 | 89.29m | 621.79o | |
| W2N1D1 | Y = 37229.95 / (1 + e7.342 - 0.075 t) | 0.964 | 98.15c | 696.20l | |
| W2N1D2 | Y = 40222.45 / (1 + e7.138 - 0.073 t) | 0.943 | 97.78d | 734.06k | |
| W2N1D3 | Y = 41942.52 / (1 + e7.029 - 0.080 t) | 0.948 | 88.08o | 836.75c | |
| W2N2D1 | Y = 40936.35 / (1 + e6.895 - 0.078 t) | 0.973 | 88.39n | 798.25f | |
| W2N2D2 | Y = 44785.55 / (1 + e6.558 - 0.077 t) | 0.950 | 85.05p | 863.24b | |
| W2N2D3 | Y = 45594.61 / (1 + e7.410 - 0.085 t) | 0.941 | 83.17q | 968.88a | |
| 显著性(P值)Significance (P value) | |||||
| 灌水水平W | — | — | * | NS | |
| 施氮水平N | — | — | * | * | |
| 种植密度D | — | — | * | * | |
| 灌水水平×施氮水平W×N | — | — | * | * | |
| 灌水水平×种植密度W×D | — | — | * | * | |
| 施氮水平×种植密度N×D | — | — | * | NS | |
| 灌水水平×施氮水平×种植密度W×N×D | — | — | * | NS | |
表3
不同处理玉米的产量、水分利用效率及氮肥利用率"
| 年份 Year | 处理 Treatment | 产量 Grain yield | 水分利用效率 WUE | 氮肥利用率 Nitrogen utilization efficiency |
|---|---|---|---|---|
| 2016 | W1N0D1 | 8015.63c | 12.18d | — |
| W1N0D2 | 8599.57c | 12.70d | — | |
| W1N0D3 | 8406.93c | 12.38d | — | |
| W1N1D1 | 12211.91b | 17.83b | 13.10d | |
| W1N1D2 | 10725.40b | 19.81a | 15.33c | |
| W1N1D3 | 11008.27b | 15.81bc | 19.30a | |
| W1N2D1 | 12037.13b | 17.19bc | 17.52f | |
| W1N2D2 | 11273.53b | 18.68ab | 20.64e | |
| W1N2D3 | 11403.40c | 16.99bc | 24.36bc | |
| W2N0D1 | 8093.53c | 12.66d | — | |
| W2N0D2 | 8247.37c | 12.69d | — | |
| W2N0D3 | 7948.39b | 12.19d | — | |
| W2N1D1 | 10471.90b | 15.55c | 12.62c | |
| W2N1D2 | 12170.47b | 17.80b | 13.92bc | |
| W2N1D3 | 11161.16b | 16.10bc | 17.84b | |
| W2N2D1 | 11238.43b | 16.40bc | 20.85f | |
| W2N2D2 | 13871.22a | 18.99ab | 22.28ef | |
| W2N2D3 | 11078.11b | 15.69c | 23.18d | |
| 2017 | W1N0D1 | 7159.1c | 11.42ef | — |
| W1N0D2 | 7605.8c | 10.74f | — | |
| W1N0D3 | 7674.9c | 12.46ef | — | |
| W1N1D1 | 9971.6cb | 20.40b | 19.29c | |
| W1N1D2 | 11329.5ab | 20.65a | 27.55a | |
| W1N1D3 | 11792.6ab | 16.02d | 23.47b | |
| W1N2D1 | 10514.2b | 17.26d | 18.86c | |
| W1N2D2 | 12297.8ab | 19.63c | 24.18a | |
| W1N2D3 | 11930.0ab | 16.10d | 21.59bc | |
| W2N0D1 | 7729.6c | 12.14ef | — | |
| W2N0D2 | 8063.5c | 13.21e | — | |
| W2N0D3 | 7708.6c | 12.07ef | — | |
| W2N1D1 | 9633.7cb | 15.31d | 16.62c | |
| W2N1D2 | 11880.5ab | 17.95cd | 27.33a | |
| W2N1D3 | 12154.3ab | 16.09d | 20.83bc | |
| W2N2D1 | 10956.1b | 17.08d | 17.42c | |
| W2N2D2 | 13699.3a | 18.29cd | 27.42a | |
| W2N2D3 | 10271.2cb | 15.80d | 20.48bc | |
| 显著性(P值)Significance (P value) | ||||
| 灌水水平W | NS | * | * | |
| 施氮水平N | * | * | * | |
| 种植密度D | * | * | * | |
| 灌水水平×施氮水平W×N | * | * | * | |
| 灌水水平×种植密度W×D | * | NS | * | |
| 施氮水平×种植密度N×D | * | * | * | |
| 灌水水平×施氮水平×种植密度W×N×D | * | * | * | |
表4
水氮耦合条件下不同密度玉米主要指标间的相关分析"
| 指标 Item | 光合速率 Pn | 干物质积累量 Dry matter accumulation | 干物质积累最大增长速率 Maximum increase rate | 水分利用效率 WUE | 籽粒产量 Grain yield |
|---|---|---|---|---|---|
| 光合速率Pn | 1 | -0.5 | 0.647** | 0.919** | 0.855** |
| 干物质积累量 Dry matter accumulation | 1 | -0.2208 | -0.259 | 0.178* | |
| 干物质积累最大增长速率 Maximum increase rate | 1 | 0.641** | 0.669** | ||
| 水分利用效率WUE | 1 | 0.898** | |||
| 籽粒产量Grain yield Grain Yield | 1 |
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