水稻花色素苷合成调节基因hrd1(t)的鉴定

doi:10.3864/j.issn.0578-1752.2013.19.001

摘要/Abstract

摘要： 【目的】对籼稻种质hrd1紫叶性状进行遗传分析和基因定位。【方法】在特籼占13与02428杂交后代中获得籼稻紫叶材料hrd1。对hrd1主要农艺性状及表型进行观察；配制hrd1与02428的F1、F2、BC1F1群体，观察紫叶与绿叶性状的分离情况，明确其遗传模式；进一步利用F2群体实现紫叶性状基因的精细定位和候选基因遴选。【结果】hrd1在2叶期的叶尖出现紫色，并逐步扩散至全株大部分组织，成熟期农艺性状与对照（特籼占13）具有较大差异。hrd1叶绿素含量正常，花色素苷含量显著高于对照。遗传分析表明其紫叶性状受1对隐性核基因hrd1(t)控制，该基因位于第4染色体InDel标记HRD10和HRD21之间，物理距离为32.5 kb。对该区域候选基因测序，发现hrd1在LOC_Os04g50660基因（编码WD和G-beta重复域蛋白）的第3外显子发生单碱基突变（A→C），导致其编码的第196位氨基酸由对照的赖氨酸变为苏氨酸。【结论】hrd1(t)编码WD40类转录因子，可能参与花色素合成调控。

关键词: 水稻, 紫叶, 花色素苷, WD40转录因子

Abstract: 【Objective】 The objective of this study is to conduct genetic analysis and gene mapping for the purple leaf character of rice germplasm hrd1 (Oryza sativa L. ssp. indica). 【Method】 The regulatory gene hrd1, is a rice germplasm with purple leaf which originated from the hybrids of two green leaf accessions Texianzhan 13 and 02428. Major agronomic traits of hrd1 was firstly investigated, and the segregation ratio of purple and green leaf characters was assayed by using the F1, F2 and BC1F1 populations derived from the crosses of hrd1 and 02428. Fine mapping for the gene contributed to purple leaf character was further conducted by using F2 population, and the candidate gene was identified. 【Result】 The purple character initiates on leaf apex of hrd1 at the 2nd leaf stage and gradually spreads to most parts of the whole plant. At the ripening stage, the agronomic trait of hrd1 was significantly different from the control cultivar (Texianzhan 13). Chlorophyll content was equal between hrd1 and the control, while the anthocyanin content in hrd1 was much higher than that in control. A recessive gene named hrd1(t) controls the purple leaf character in hrd1 according to genetic analysis, and it was mapped to a 32.5 kb region on chromosome 4 between InDel markers HRD10 and HRD21. By sequencing for genes within the mapped region, a single base mutation (A to C) was found in the 3rd exon of LOC_Os04g50660 (encoding a WD and G-beta repeats containing protein), which leads to the predicted amino acid at position 196 converts from lysine in control to threonine in hrd1. 【Conclusion】hrd1(t) encodes a WD40-class transcription factor and may be involved in a regulatory mechanism of pigments.

Key words: rice , purple leaf trait , anthocyanin , WD40 regulatory gene

刘永柱, 罗文龙, 黄翠红, 陈立凯, 王慧, 陈志强, 郭涛. 水稻花色素苷合成调节基因hrd1(t)的鉴定[J]. 中国农业科学, 2013, 46(19): 3955-3964.

LIU Yong-Zhu, LUO Wen-Long, HUANG Cui-Hong, CHEN Li-Kai, WANG Hui, CHEN Zhi-Qiang, GUO Tao. Characterization of the Regulatory Gene hrd1(t) Involved in Anthocyanin Biosynthesis[J]. Scientia Agricultura Sinica, 2013, 46(19): 3955-3964.

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