芝麻栽培种与野生种（Sesamum schinzianum Asch、Sesamum radiatum Schum & Thonn）种间杂交后代的生物学特性

doi:10.3864/j.issn.0578-1752.2013.19.002

摘要/Abstract

摘要： 【目的】探明芝麻栽培种与野生种种间杂交的亲和性，分析杂交后代遗传特征。【方法】以26个基因型（Sesamum indicum L.）与刚果野芝麻（Sesamum schinzianum Asch）和野芝1号（Sesamum radiatum Schum & Thonn）野生种为杂交亲本，借助胚培养技术获得种间杂交后代。采用SSR标记和生物学方法鉴定并分析杂交种F1的遗传特性。【结果】通过胚培养技术获得2 430个种间杂交F1株系，对部分材料的检测表明杂种阳性率为95.83%。刚果野芝麻×栽培种的正反交杂交率分别为34.62%（A）和11.54%（C），野芝1号×栽培种的正反交杂交率分别为100%（B）和19.23%（D）。F1花粉粒存在部分不育和高度不育两种类型，F1自交结实率分别为2.32—2.57粒/蒴果（S. schinzianum×S. indicum）和0.30—2.45粒/蒴果（S. radiatum ×S. indicum）。【结论】S. radiatum与栽培种的杂交亲和率高于S. schinzianum。以野生种为母本与栽培种杂交，杂交种F1在株高、根系结构等性状方面超亲表现明显；株系高抗枯萎病；部分F1株系有低自交结实性。

关键词: 芝麻 , 种间杂交 , 鉴定 , 枯萎病抗性 , 遗传

Abstract: 【Objective】 In order to explore the compatibility of interspecific hybridization between Sesamum indicum L. and wild species of Sesamum, the genetic characteristics of F1 interspecific hybrids were analyzed. 【Method】Twenty-six cultivar accessions (S. indicum L.) and two wild species, i.e. Congo wild sesame (S. schinzianum Asch) and No. 1 wild sesame (S. radiatum Schum & Thonn) were used as cross parents, the F1 interspecific hybrid progenies were obtained using the immature embryo culture technique. The genetic characters of F1 hybrids were assessed and analyzed using the SSR marker polymorphism and biologic method. 【Result】A total of 2,430 lines of F1 interspecific hybrids were obtained through immature embryo rescue, and the ratio of true hybrid lines reached 95.83%. The crossability frequencies of ‘Congo wild sesame×S. indicum’and ‘No. 1 wild sesame×S. indicum’ cross combinations were 42.31% (direct cross), 11.54% (reciprocal cross), 100% (direct cross), and 19.23% (reciprocal cross), respectively. Pollens of F1 interspecific hybrids presented the partial sterility and high sterility types, and the ratios of F2 seed setting in ‘S. schinzianum×S. indicum’ and ‘S. radiatum ×S. indicum’ crosses were 2.32-2.57 and 0.30-2.45 viable seeds per capsule, respectively. 【Conclusion】The crossability ratio of the cross between S. radiatum and S. indicum is higher than that of the cross between S. schinzianum and S. indicum. F1 hybrids show an obvious superiority for the biological characters such as plant height and root structure, and presented a high resistance to Fusarium wilt disease. Partial F1 lines have the low ratios of viable seed setting.

Key words: sesame (Sesamum indicum L. , Sesamum radiatum , Sesamum schinzianum) , interspecific hybrid , identification , Fusarium wilt resistance , genetics

张海洋, 苗红梅, 张体德, 魏利斌, 李春, 王慧丽, 段迎辉, 琚铭. 芝麻栽培种与野生种（Sesamum schinzianum Asch、Sesamum radiatum Schum & Thonn）种间杂交后代的生物学特性[J]. 中国农业科学, 2013, 46(19): 3965-3977.

ZHANG Hai-Yang, MIAO Hong-Mei, ZHANG Ti-De, WEI Li-Bin, LI Chun, WANG Hui-Li, DUAN Ying-Hui, JU Ming. Biological Characters of Interspecific Hybrid Progenies Between Sesamum indicum L. and Wild Relatives (Sesamum schinzianum Asch, Sesamum radiatum Schum & Thonn)[J]. Scientia Agricultura Sinica, 2013, 46(19): 3965-3977.

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参考文献

[1]张海洋, 郑永战, 卫双玲. 我国芝麻产业现状与发展对策//全国芝麻和特油作物产业发展暨学术研讨会论文汇编. 2007: 9-18.

Zhang H Y, Zheng Y Z, Wei S L. Sesame industry status and development strategy in China//China sesame and Specific Oil Crops Industry Development and Academic Research Discussion Meeting Papers Edition. 2007: 9-18. (in Chinese)

[2]张海洋, 卫双玲, 郑永战, 张体德, 卫文星. 野生芝麻及其抗病耐渍性状利用研究初报. 河南农业科学, 2001, 10: 15-16.

Zhang H Y, Wei S L, Zheng Y Z, Zhang T D, Wei W X. Preliminary studies on wild sesame and its utilization. Journal of Henan Agricultural Sciences, 2001, 10: 15-16. (in Chinese)

[3]Baydar H. Breeding for the improvement of the ideal plant type of sesame. Plant Breeding, 2008, 124(3): 263-267.

[4]Mensah J K, Obadoni B O, Eruotor P G, Onome-Irieguna F. Simulated flooding and drought effects on germination, growth, and yield parameters of sesame (Sesamum indicum L.). African Journal of Biotechnolology, 2006, 5(13): 1249-1253.

[5]Ashri A. Sesame (Sesamum indicum L.) // Singh R J. Genetics Resources, Chromosome Engineering, and Crop Improvement. Boca Raton: CRC Press, USA, 2007, 4: 231-290.

[6]El-Bramawy M A S, Shaban W I. Nature of gene action for yield, yield components and major diseases resistance in sesame (Sesamum indicum L.). Research Journal of Agriculture and Biological Sciences, 2007, 3(6): 821-826.

[7]Yol E, Uzun B. Geographical patterns of sesame accessions grown under Mediterranean environmental conditions, and establishment of a core collection. Crop Science, 2012, 52(5): 2206-2214.

[8]孙建, 张秀荣, 张艳欣, 车卓, 黄波. 中国芝麻主要品种遗传多样性特点及遗传基础演变. 中国农业科学, 2009, 42(10): 3421-3431.

Sun J, Zhang X R, Zhang Y X, Che Z, Huang B. Analysis on genetic diversity and genetic basis of main sesame cultivars released in China. Scientia Agricultura Sinica, 2009, 42(10): 3421-3431. (in Chinese)

[9]岳文娣, 魏利斌, 张体德, 李春, 苗红梅, 张海洋. 芝麻种质资源SSR标记遗传多样性与群体结构分析. 作物学报, 2012, 38(12): 2286-2296.

Yue W D, Wei L B, Zhang T D, Li C, Miao H M, Zhang H Y. Genetic diversity and population structure of germplasm resources in sesame (Sesamum indicum L.) by SSR markers. Acta Agronomica Sinica, 2012, 38(12): 2286-2296. (in Chinese)

[10]Joshi A B. Sesamum. Hyderabad: Examiner Press, 1961.

[11]Kulkarni V V. Studies on interspecific hybridization with particular reference to development of male sterility in sesame (Sesamum indicum L.) [D]. Dharwad: University of Agricultural Sciences, India, 2006.

[12]Nimmakayala P, Perumal R, Mulpuri S, Reddy U K. Sesamum// Kole C. Wild Corp Relatives: Genomic and Breeding Resources Oilseeds. Berlin Heidelberg: Springer-Verlag Press, 2011: 261-273.

[13]Nayar N M, Mehra K L. Sesame: Its uses, botany, cytogenetics and origin. Economic Botany, 1970, 24(1): 20-31.

[14]Kobayashi T. Cytogenetics of sesame (Sesamum indicum). Tsuchi Y A T, Gupta P K. Chromosome Engineering in Plants: Genetics Breeding, Evolution. Amsterdam: Elseiver press, Netherlands, 1991: 581-592.

[15]Tarihal R, Sridevi O, Shenoy V V, Salimath P M. Study of fertilization barriers in crosses between Sesamum indicum and its wild relatives. The Indian Journal of Genetics and Plant Breeding, 2003, 63(2): 132-136.

[16]Lee J I, Lee B H, Seong N S, Kang C W. Studies on interspecific hybridization in sesame, 1: Characteristics and cross affinity of wild sesame. Korean Journal of Breeding, 1991, 22(4): 356-360.

[17]石淑稳. 芝麻野生种与栽培种的交配能力. 中国油料, 1993, 2: 18-21.

Shi S W. The crossability between wild and cultivated species in sesame. Chinese Oil Crops, 1993, 2: 18-21.

[18]瞿桢, 吴新镛, 夏伏建. 芝麻远缘杂种胚胎的营救和植株再生. 中国油料, 1994, 16(1): 33-35.

Qu Z, Wu X Y, Xia F J. Embryo rescue and plant regeneration of interspecific hybridization between S. indicum and a wild species S. schinzianum. Chinese Oil Crops, 1994, 16(1): 33-35. (in Chinese)

[19]Kulkarni V, Sridevi O. Production of interspecific hybrids through ovule culture in sesame. International Journal of Plant Sciences, 2009, 4(1): 128-130.

[20]Rajeswari S, Thiruvengadam V, Ramaswamy N M. Production of interspecific hybrids between Sesamum alatum Thonn and Sesamum indicum L. through ovule culture and screening for phyllody disease resistance. South African Journal of Botany, 2010, 76(2): 252-258.

[21]Prabakaran A J. Cytoplasmic male sterility in sesame from the species cross//Martínez J F. Sesamum malabaricum×S. indicum: Ⅰ. Substitution Backcrosses. Sesame and Safflower Newsletter. Córdoba: IAS Press, Spain, 1998: 1-6.

[22]Ram S G, Sundaravelpandian K, Kurnar M, Vinod K K, Bapu J P K, Raveendran T S. Pollen-pistil interaction in the inter-specific crosses of Sesamum sp. Euphytica, 2006, 152: 379-385.

[23]Paterson A H, Brubaker C, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Molecular Biology Reporter, 1999, 11(2): 122-127.

[24]苗红梅, 仇存璞, 魏利斌, 杜华, 张海洋. 一种尖孢镰刀菌芝麻专化型致病力的鉴定方法: 中国, CN102487630A. 2012-06-03 [2013-06-18].

Miao H M, Qiu C P, Wei L B, Du H, Zhang H Y. An evaluation method of the pathogenicity of Fusarium oxysporum f. sp. sesami: China, CN102487630A. 2012-06-03[2013-06-18]. (in Chinese).

[25]Peterson R, Slovin J, Chen C. A simplified method for differential staining of aborted and non-aborted pollen grains. International Journal of Plant Biology, 2010, 1(3): e13.

[26]Biwas A K, Mitra A K. Interspecfic hybridization in three species of Sesamum. Indian Journal of Genetics and Plant Breeding, 1990, 50, 4: 307-309.

[27]Kumar L S, Abraham A. A cytological study of sterility in Sesamum orientale L.. Indian Journal Genetics, 1941, 7: 41-60.

[28]王文泉, 郑永战, 柳家荣, 屠礼传, 梁秀银. 芝麻雄性核不育两系制种效果的研究. 中国油料, 1995, 17(1): 12-15.

Wang W Q, Zheng Y Z, Liu J R, Tu L C, Liang X Y. The effect of hybrid seed production by utilizing genetic male sterile lines in sesame. Chinese Oil Crops, 1995, 17(1): 12-15. (in Chinese)

[29]Kumar S T, Thangavelu S, Rangasamy S R S. Heterosis and combining ability for seed size characters in sesame (Sesamum indicum L.) // Martínez J F. Sesame and Safflower Newsletter. Córdoba: CSIC Press, 1998: 33-42.

[30]Kulkarni V, Sridevi O. In vivo studies on pollen germination, tube growth and micropylar penetration in interspecific crosses of sesame (Sesamum indicum L.). International Journal of Plant Sciences, 2009, 4(1): 250-254.

[31]刘红艳, 赵应忠. 芝麻栽培种与野生种种间杂交亲和性研究. 中国农学通报, 2011, 27(9): 156-159.

Liu H Y, Zhao Y Z. Studies on the hybridization compatibility between cultivated sesame and its wild species. Chinese Agricultural Science Bulletin, 2011, 27(9): 156-159. (in Chinese)

[32]张海洋, 苗红梅, 李春, 魏利斌, 马琴. 芝麻染色体核型及似近系数分析. 植物学报, 2012, 47(6): 602-614.

Zhang H Y, Miao H M, Li C, Wei L B, Ma Q. Analysis of sesame karyotype and resemblance-near coefficient. Chinese Bulletin of Botany, 2012, 47(6): 602-614. (in Chinese)

[33]Arasu N T. Self-incompatiblity in angiosperms: A review. Genetica, 1968, 39(1): 1-24.

[34]Kulkarni V V, Sridevi O, Salimath P M, Parameshwarappa K G. Cytoplasmic and nuclear genome interaction in interspecific crosses of sesame (Sesamum indicum L.). Indian Journal of Genetic and Plant Breeding, 2011, 71(2): 174-179.