白叶枯病菌和细菌性条斑病菌多样性的TALE效应 蛋白调控水稻抗（感）病性机理与利用策略

doi:10.3864/j.issn.0578-1752.2013.14.005

摘要/Abstract

摘要： 培育和种植抗病品种是防治水稻白叶枯病和条斑病的有效措施。最近几年有关TALE效应蛋白调控水稻抗（感）病性研究取得了突破性进展，这将改变水稻抗性品种培育策略。为此，本文对稻黄单胞菌-水稻互作系统中已知TALE效应蛋白与水稻中的已知或未知抗（感）病基因（R或S）的对应关系进行了归纳，并就tale基因进化及对应水稻R或S基因发掘进行了分析。另外，文中还对以TALE为基础发展而来的TALEN技术遗传修饰水稻感病基因的前景进行了展望。利用TALEN技术可将高产优质但感病的水稻品种（材料）转变为高产优质兼广谱抗病的水稻品种（材料）。

关键词: 水稻 , 稻黄单胞菌 , TALE效应蛋白 , 抗（感）病机理 , TALEN

Abstract: Breeding and planting resistant rice varieties is one of the most effective and economic ways to control bacterial leaf blight and bacterial leaf streak diseases caused by Xanthomonas oryzae pv. oryzae (Xoo) and pv. oryzicola (Xoc), respectively. Recent land-marked advances in understanding the mechanism of rice resistance (R) or susceptibility(S) manipulated by transcriptional activator-like effector (TALEs) of Xoo and Xoc shall definitely alter present strategies in rice breeding for disease resistance. Hereby, the authors reviewed the corresponding relationships between known TALEs of a Xoo model strain PXO99A and known and unknown R or S genes in rice, forwarded the prospects regarding to co-evolution of new tale genes in the pathogens and to mine R or S genes in rice, and introduced a TALEN technology, developed based on DNA-binding specificity of TALEs, that has been used to genetically transfer a susceptible rice line into a resistant one. The TALEN technology is tremendously a powerful tool that will realize such a goal in altering susceptible rice varieties (materials) with high yield and high quality into resistant ones with high yield and high quality.

Key words: rice , Xanthomonas oryzae , TALE effector , resistance (susceptibility) mechanism , TALEN

李争, 熊鹂, 纪志远, 邹丽芳, 邹华松, 陈功友. 白叶枯病菌和细菌性条斑病菌多样性的TALE效应蛋白调控水稻抗（感）病性机理与利用策略[J]. 中国农业科学, 2013, 46(14): 2894-2901.

LI Zheng, XIONG Li, JI Zhi-Yuan, ZOU Li-Fang, ZOU Hua-Song, CHEN Gong-You. Mechanisms of Rice Resistance (Susceptibility) Manipulated by Diverse TALEs of Xanthomonas oryzae pv. oryzae and pv. oryzicola and Potential Utilization in Rice Breeding[J]. Scientia Agricultura Sinica, 2013, 46(14): 2894-2901.

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