水稻白叶枯病菌内源过氧化氢在细胞分裂周期中的时空定位变化

doi:10.3864/j.issn.0578-1752.2012.08.005

中国农业科学 ›› 2012, Vol. 45 ›› Issue (8): 1499-1504.doi: 10.3864/j.issn.0578-1752.2012.08.005

水稻白叶枯病菌内源过氧化氢在细胞分裂周期中的时空定位变化

李欣, 李鑫玲, 庞新跃, 朱文学, 樊金玲, 罗磊, 杜琳, 王娜, 王利平

1.河南科技大学食品与生物工程学院，河南洛阳 471003
2.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京100193
3.河南科技大学医学技术与工程学院，河南洛阳 471003

收稿日期:2011-08-25 出版日期:2012-04-15 发布日期:2011-09-20
通讯作者: 通信作者朱文学，Tel：0379-64282798；E-mail：zwx@mail.haust.edu.cn
作者简介:李欣，Tel/Fax：0379-64282342；E-mail：lixinpxy@hotmail.com
基金资助:
国家自然科学基金（31000017，30670070）、中国博士后科学基金（20110490046）、植物病虫害生物学国家重点实验室开放基金（SKL2011OP06）

Spatio-Temporal Localization Changes of Endogenous Hydrogen Peroxide During Cell Division Cycle of Xanthomonas oryzae pv. oryzae

LI Xin, LI Xin-Ling, PANG Xin-Yue, ZHU Wen-Xue, FAN Jin-Ling, LUO Lei, DU Lin, WANG Na, WANG Li-Ping

1.河南科技大学食品与生物工程学院，河南洛阳 471003
2.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京100193
3.河南科技大学医学技术与工程学院，河南洛阳 471003

Received:2011-08-25 Online:2012-04-15 Published:2011-09-20

摘要/Abstract

摘要： 【目的】分析细菌内源过氧化氢在细胞分裂周期中的时空变化，探讨细菌增殖中过氧化氢的生理功能。【方法】采用组织化学法通过透射电子显微镜技术观察水稻白叶枯病菌内源过氧化氢的积累位置。【结果】不同菌株内源过氧化氢水平不同，在细菌的整个细胞分裂周期中观察到过氧化氢在细胞壁上的积累水平维持稳定，在多个菌株的细胞分裂过程中，除细胞壁之外，还在2个新的位点（类间体结构和核区）出现过氧化氢的大量积累,表现出数量和空间定位的显著变化。【结论】在多个水稻白叶枯病菌菌株中发现胞内额外的大量过氧化氢积累和定位与细胞分裂的进程密切相关，这种过氧化氢的时空变化很可能是细菌分裂时的普遍现象。推测过氧化氢应在细菌增殖中具有重要作用。

关键词: 细胞分裂, 过氧化氢, 定位, 类间体结构, 核区, 水稻白叶枯病菌

Abstract: 【Objective】The objective of this study is to investigate the changes of spatio-temporal localization of endogenous hydrogen peroxide (H2O2) in strains of Xanthomonas oryzae pv. oryzae, and to illustrate the physiological function of H2O2 in bacterial proliferation. 【Method】 Histochemical methods through transmission electron microscope were used to observe localization of endogenous H2O2. 【Result】 Even though the levels of endogenous H2O2 production were different among various strains, the produced H2O2 was localized in the cell wall of all Xanthomonas oryzae pv. oryzae strains tested. Interestingly, the accumulated H2O2 was also localized in the mesosome-like structure and nucleoids during the cell division cycle of strains. Furthermore, results revealed the quantitative and dimensional changes of H2O2 accumulation in the two additional locations. 【Conclusion】Additional locations of the accumulated H2O2 are closely associated with the process of cell division of all Xanthomonas oryzae pv. oryzae strains investigated. Spatio-temporal localization changes of endogenous H2O2 should be an ubiquitous phenomenon during cell division cycle of bacterium. H2O2 may play key roles in bacterial proliferation.

Key words: cell division, hydrogen peroxide, localization, mesosome-like structure, nucleoid, Xanthomonas oryzae pv. oryzae

李欣, 李鑫玲, 庞新跃, 朱文学, 樊金玲, 罗磊, 杜琳, 王娜, 王利平. 水稻白叶枯病菌内源过氧化氢在细胞分裂周期中的时空定位变化[J]. 中国农业科学, 2012, 45(8): 1499-1504.

LI Xin, LI Xin-Ling, PANG Xin-Yue, ZHU Wen-Xue, FAN Jin-Ling, LUO Lei, DU Lin, WANG Na, WANG Li-Ping. Spatio-Temporal Localization Changes of Endogenous Hydrogen Peroxide During Cell Division Cycle of Xanthomonas oryzae pv. oryzae[J]. Scientia Agricultura Sinica, 2012, 45(8): 1499-1504.

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水稻白叶枯病菌内源过氧化氢在细胞分裂周期中的时空定位变化

Spatio-Temporal Localization Changes of Endogenous Hydrogen Peroxide During Cell Division Cycle of Xanthomonas oryzae pv. oryzae

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