中国农业科学 ›› 2011, Vol. 44 ›› Issue (20): 4295-4301.doi: 10.3864/j.issn.0578-1752.2011.20.018

• 畜牧·资源昆虫 • 上一篇    下一篇

胰岛素样生长因子-I对鸡等级前卵泡发育的促进作用

贾玉东, 颜菲菲, 曾卫东, 张才乔   

  1. 1.浙江大学动物科学学院
  • 收稿日期:2010-08-20 出版日期:2011-10-15 发布日期:2010-12-30
  • 通讯作者: 通信作者张才乔,Tel:0571-88982976;E-mail:cqzhang@zju.edu.cn
  • 作者简介:贾玉东,E-mail:jydspeed456@163.com
  • 基金资助:

    国家自然科学基金项目(30871843)、浙江省科技厅项目(2008C22040)

Promoting Effect of IGF-I on Prehierarchical Follicle Development in Laying Chickens

 JIA  Yu-Dong, YAN  Fei-Fei, ZENG  Wei-Dong, ZHANG  Cai-Qiao   

  1. 1.浙江大学动物科学学院
  • Received:2010-08-20 Online:2011-10-15 Published:2010-12-30

摘要: 【目的】探讨胰岛素样生长因子-I(IGF-I)对产蛋鸡等级前卵泡发育的影响。【方法】用免疫组化和RT-PCR法检测IGF-I受体在鸡等级前卵泡中的表达,研究IGF-I对鸡早期卵泡发育的影响。【结果】IGF-I受体在等级前卵泡颗粒层和膜层均有表达,且随着卵泡的发育其表达水平逐渐增高,在小黄卵泡(SYF)阶段达到最大;同时,卵泡体外培养结果表明IGF-I(100 ng•mL-1)、FSH(10 n•mL-1)及IGF-I+FSH处理可显著增加SYF中膜层和颗粒层的厚度及细胞密度,且IGF受体在颗粒层和膜层中的表达水平也显著增加,其中以IGF-I+FSH联合处理组的效果最为显著。【结论】IGF-I能显著提高产蛋鸡SYF颗粒层和膜层的细胞数目以及厚度,同时增加IGF-I受体的表达,并可与FSH联合作用以提高IGF受体的表达,促进鸡卵泡细胞的增殖,进而调节鸡等级前卵泡的发育,此结果有助于揭示家禽卵泡发育的局域性内分泌调节作用。

关键词: 胰岛素样生长因子-I, 等级前卵泡, 卵泡发育,

Abstract: 【Objective】The aim of the experiment is to investigate the effect of IGF-I on prehierarchical follicle development from laying chickens.【Method】IGF-I receptor (IGF-IR) expression in granulosa and theca cells was studied in the prehierarchical follicles of laying chickens via immunohistochemistry and RT-PCR. In addition, the effects of IGF-I on small yellow follicle (SYF) development were investigated. 【Result】The expression of IGF-IR gradually increased in both granulosa and theca layers from the prehierarchical follicles. Moreover, IGF-IR displayed the highest expression in SYF. Compared with control, treatment with IGF-I and FSH alone or in combination significantly increased cell density, thickness of granulosa and theca layers, stimulated the expression IGF-IR in both granulosa and theca layer from SYF. Furthermore, IGF-I and FSH displayed an additive effect. 【Conclusion】 These results indicate that IGF-I may increase follicular cell number, thickness of granulosa and theca layers and stimulated IGF-IR mRNA expression in SYF. In addition, IGF-I exhibited synergistic effect with FSH to enhance prehierarchical follicular cell proliferation. Therefore, these results may facilitate delineating the mechanism of intra-ovarian endocrine regulation of poultry follicle development.

Key words: IGF-I, prehierarchicalfollicle, follicledevelopment, chicken