胰岛素样生长因子-I对鸡等级前卵泡发育的促进作用

doi:10.3864/j.issn.0578-1752.2011.20.018

摘要/Abstract

摘要： 【目的】探讨胰岛素样生长因子-I（IGF-I）对产蛋鸡等级前卵泡发育的影响。【方法】用免疫组化和RT-PCR法检测IGF-I受体在鸡等级前卵泡中的表达，研究IGF-I对鸡早期卵泡发育的影响。【结果】IGF-I受体在等级前卵泡颗粒层和膜层均有表达，且随着卵泡的发育其表达水平逐渐增高，在小黄卵泡(SYF)阶段达到最大；同时，卵泡体外培养结果表明IGF-I（100 ng•mL-1）、FSH（10 n•mL-1）及IGF-I+FSH处理可显著增加SYF中膜层和颗粒层的厚度及细胞密度，且IGF受体在颗粒层和膜层中的表达水平也显著增加，其中以IGF-I+FSH联合处理组的效果最为显著。【结论】IGF-I能显著提高产蛋鸡SYF颗粒层和膜层的细胞数目以及厚度，同时增加IGF-I受体的表达，并可与FSH联合作用以提高IGF受体的表达，促进鸡卵泡细胞的增殖，进而调节鸡等级前卵泡的发育，此结果有助于揭示家禽卵泡发育的局域性内分泌调节作用。

关键词: 胰岛素样生长因子-I, 等级前卵泡, 卵泡发育, 鸡

Abstract: 【Objective】The aim of the experiment is to investigate the effect of IGF-I on prehierarchical follicle development from laying chickens.【Method】IGF-I receptor (IGF-IR) expression in granulosa and theca cells was studied in the prehierarchical follicles of laying chickens via immunohistochemistry and RT-PCR. In addition, the effects of IGF-I on small yellow follicle (SYF) development were investigated. 【Result】The expression of IGF-IR gradually increased in both granulosa and theca layers from the prehierarchical follicles. Moreover, IGF-IR displayed the highest expression in SYF. Compared with control, treatment with IGF-I and FSH alone or in combination significantly increased cell density, thickness of granulosa and theca layers, stimulated the expression IGF-IR in both granulosa and theca layer from SYF. Furthermore, IGF-I and FSH displayed an additive effect. 【Conclusion】 These results indicate that IGF-I may increase follicular cell number, thickness of granulosa and theca layers and stimulated IGF-IR mRNA expression in SYF. In addition, IGF-I exhibited synergistic effect with FSH to enhance prehierarchical follicular cell proliferation. Therefore, these results may facilitate delineating the mechanism of intra-ovarian endocrine regulation of poultry follicle development.

Key words: IGF-I, prehierarchicalfollicle, follicledevelopment, chicken

贾玉东, 颜菲菲, 曾卫东, 张才乔. 胰岛素样生长因子-I对鸡等级前卵泡发育的促进作用[J]. 中国农业科学, 2011, 44(20): 4295-4301.

JIA Yu-Dong, YAN Fei-Fei, ZENG Wei-Dong, ZHANG Cai-Qiao. Promoting Effect of IGF-I on Prehierarchical Follicle Development in Laying Chickens[J]. Scientia Agricultura Sinica, 2011, 44(20): 4295-4301.

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