核桃亲子鉴定方法的建立

doi:10.3864/j.issn.0578-1752.2011.20.013

摘要/Abstract

摘要： 【目的】针对核桃育种中父本不清、亲本混淆问题，建立简便快捷高效的核桃亲本鉴定方法，也为其它林木和果树品种亲本鉴定提供参考。【方法】以‘鲁果2号’为鉴定材料，采用表型性状和SSR标记的方法对‘鲁果2号’及其可能的亲本进行亲子分析。【结果】表型性状方法统计的8对坚果性状在各品种间差异很小，不能鉴定出子代亲本；SSR分型检测出疑似亲本中只有‘上宋6号’和‘鲁香’与子代‘鲁果2号’的等位基因遗传符合孟德尔遗传规律，其累积RCP（亲子关系概率）为99.997%，‘slz-40’与已知亲本鉴定结果与实际相符。【结论】‘上宋6号’和‘鲁香’是‘鲁果2号’的亲本，SSR分型技术较好地鉴别出了‘鲁果2号’的亲本，为其它林木和果树品种寻找和鉴别亲本，提供有效手段。

关键词: 核桃, 表型性状, SSR, 亲权指数, 亲子鉴定

Abstract: 【Objective】A simple, rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding ,which provided reference for other varieties. 【Method】'Luguo-2' was used as material. Phenotypic traits and SSR markers were conducted to analyse parentage relationship between 'Luguo-2' and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results. In genotype results, the heredity of 'Shangsong-6', 'Luxiang' and 'Luguo-2' accords with Mendelian genetic regularity, there may be a paternity. PI and RCP wre analyzed by using the results of 10 SSR primer pairs, and the cumulative RCP was 99.997%. In addition, the appraisal result between ‘Slz-40’ and its known parent was consistent with the reality. 【Conclusion】'Shangsong-6' and 'Luxiang' were the parents of 'Luguo-2' with SSR clustering results supporting the conclusion. The parents of 'Luguo-2' were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.

Key words: walnut, phenotypes, SSR, PI, paternity testwalnut, phenotypes, SSR, PI, paternitytest

周贝贝, 马庆国, 王滑, 王伟, 张美勇, 裴东. 核桃亲子鉴定方法的建立[J]. 中国农业科学, 2011, 44(20): 4258-4264.

ZHOU Bei-Bei, MA Qing-Guo, WANG Hua, WANG Wei, ZHANG Mei-Yong, PEI Dong. Establishment of Walnut Paternity Test[J]. Scientia Agricultura Sinica, 2011, 44(20): 4258-4264.

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