中国农业科学 ›› 2025, Vol. 58 ›› Issue (8): 1650-1662.doi: 10.3864/j.issn.0578-1752.2025.08.015

• 研究简报 • 上一篇    

基于红蓝光调控分析VvBES1-1参与‘红地球’葡萄花芽的分化

汤学燊(), 党仕卓, 周娟, 李佳豪, 李梅花, 胡豪, 张亚红()   

  1. 宁夏大学葡萄酒与园艺学院,银川 750021
  • 收稿日期:2024-09-09 接受日期:2024-12-23 出版日期:2025-04-16 发布日期:2025-04-21
  • 通信作者:
    张亚红,E-mail:
  • 联系方式: 汤学燊,E-mail:986616585@qq.com。
  • 基金资助:
    宁夏回族自治区重点研发计划(2021BEF2016); 宁夏回族自治区重点研发计划(2023BCF1054)

Analysis of VvBES1-1 Involvement in Flower Bud Differentiation of Red Globe Grape Based on Red and Blue Light Regulation

TANG XueShen(), DANG ShiZhuo, ZHOU Juan, LI JiaHao, LI MeiHua, HU Hao, ZHANG YaHong()   

  1. School of Enology and Horticulture, Ningxia University, Yinchuan 750021
  • Received:2024-09-09 Accepted:2024-12-23 Published:2025-04-16 Online:2025-04-21

摘要:

【目的】BRI1-EMS-Suppressor 1(BES1)是油菜素内酯(BR)信号传导的重要转录因子,调节植物光形态建成,并参与光周期开花。研究红光和蓝光调控下BES1在‘红地球’葡萄花芽分化中的功能,为解析红光和蓝光介导的BR调控机制奠定基础,也为研究其他木本果树的开花调控提供参考。【方法】应用生物信息学分析VvBES1-1蛋白结构及序列比对。以‘红地球’葡萄花芽为试材,以温室自然光为对照(CK),设置红蓝4﹕1(R4B1)为光照处理,采集‘红地球’葡萄花芽,利用qPCR分析VvBES1-1在‘红地球’葡萄花芽分化过程中的时空表达模式和组织特异性。运用转化烟草方法进行亚细胞定位。通过双分子荧光互补(BiFC)试验探究VvBES1-1与其他蛋白的相互作用,以及运用酵母自激活活性测定评估VvBES1-1的转录激活能力。【结果】VvBES1-1具有BES1_N结构域,是一种BES1-S型蛋白,与毛果杨BES1亲缘关系最近,在‘红地球’葡萄各时期均有表达。在R4B1处理期间,与对照相比,VvBES1-1表达量显著降低。在花序次轴发育期,VvBES1-1表达量达到最高,此外,经10 mmol·L-1EBR处理后,促进了‘红地球’葡萄花芽VvBES1-1的表达。酵母自激活试验表明,VvBES1-1具有自激活活性。亚细胞定位结果表明,VvBES1-1定位于细胞核。过表达VvBES1-1烟草延迟开花时间,促进茎的伸长,并增加了分生组织的数量。VvBES1-1的下调表达则整合了BR和光周期信号,促进了‘红地球’葡萄花芽的分化。在‘红地球’葡萄花芽分化过程中,VvBES1-1受红光和蓝光的显著调节,且在R4B1处理下,VvBES1-1的表达水平在10 mmol·L-1的EBR处理6 h时达到最高。【结论】VvBES1-1在‘红地球’葡萄花芽分化中起重要作用。VvBES-1能够整合BR与光周期信号抑制葡萄花芽分化。而在红蓝光的影响下,能够有效下调VvBES-1的表达,达到促进葡萄花芽分化的目的。

关键词: ‘红地球’葡萄, VvBES1-1, 红蓝光, BR, 花芽分化

Abstract:

【Objective】 BRI1-EMS-Suppressor 1 (BES1), a key transcription factor in brassinosteroid (BR) signaling, regulates plant photomorphogenesis and photoperiodic flowering. This study aimed to investigate the role of BES1 in flower bud differentiation of Vitis vinifera Red Globe under red and blue light regulation, thereby elucidating the BR-mediated mechanisms driven by light quality and providing insights into flowering regulation in other woody fruit trees.【Method】 Bioinformatics analysis was performed to characterize the protein structure and sequence alignment of VvBES1-1. Flower buds of Red Globe grape were collected under greenhouse natural light (CK, control) and red:blue=4:1 (R4B1) light treatment. qPCR was used to analyze the spatiotemporal expression patterns and tissue specificity of VvBES1-1 during flower bud differentiation. Subcellular localization was determined via tobacco (Nicotiana benthamiana) transient transformation. Protein-protein interactions were examined using bimolecular fluorescence complementation (BiFC), and transcriptional activation activity was assessed via yeast autoactivation assays. 【Result】VvBES1-1 contains a BES1_N domain and belongs to the BES1-S-type protein family, showing the closest phylogenetic relationship with Populus trichocarpa BES1. It is expressed throughout all developmental stages of Red Globe grapevines. During R4B1 treatment, the expression level of VvBES1-1 was significantly reduced compared to the control. Its expression peaked during the development of secondary inflorescence axes. Additionally, treatment with 10 mmol·L-¹ EBR enhanced VvBES1-1 expression in Red Globe grape flower buds. Yeast autoactivation assays demonstrated that VvBES1-1 possesses self-activation activity. Subcellular localization analysis revealed that VvBES1-1 is localized in the nucleus. Overexpression of VvBES1-1 in tobacco delayed flowering time, promoted stem elongation, and increased meristem number. Downregulation of VvBES1-1 expression integrated brassinosteroid (BR) and photoperiod signaling pathways to promote flower bud differentiation in Red Globe grapes. During flower bud differentiation, VvBES1-1 expression was significantly regulated by red and blue light. Under R4B1 treatment, its expression peaked after 6 hours of 10 mmol·L-¹ EBR exposure.【Conclusion】VvBES1-1 plays a critical role in flower bud differentiation of Red Globe grapes. It integrates BR and photoperiod signals to inhibit grape flower bud differentiation. However, under red and blue light conditions, the expression of VvBES1-1 is effectively downregulated, thereby promoting flower bud differentiation. This study provides insights into the regulatory mechanisms of light and phytohormones in grape reproductive development.

Key words: Red Globe grape, VvBES1-1, red and blue light, BR, bud differentiation