秸秆还田配施化肥对土壤生态系统多功能性的影响

doi:10.3864/j.issn.0578-1752.2025.14.009

中国农业科学 ›› 2025, Vol. 58 ›› Issue (14): 2821-2837.doi: 10.3864/j.issn.0578-1752.2025.14.009

• 土壤肥料·节水灌溉·农业生态环境 • 上一篇下一篇

秸秆还田配施化肥对土壤生态系统多功能性的影响

宣泽鹏¹^,²(), 冯慧瑶¹^,², 陈美淇²^,³, 徐基胜², 刘梦璇²^,⁵, 赵炳梓²^,⁴^,^*(), 张佳宝¹^,²^,^*()

¹ 山西农业大学资源环境学院，山西太谷 030801
² 土壤与农业可持续发展国家重点实验室（中国科学院南京土壤研究所），南京 211135
³ 南京师范大学地理科学学院，南京 210023
⁴ 中国科学院大学南京学院，南京 211135
⁵ 南京林业大学林草学院、水土保持学院，南京 210037

收稿日期:2024-09-14 接受日期:2024-11-02 出版日期:2025-07-17 发布日期:2025-07-17
通信作者:
赵炳梓，E-mail：bzhao@issas.ac.cn。
张佳宝，E-mail：jbzhang@issas.ac.cn。
联系方式: 宣泽鹏，E-mail：1372298027@qq.com。
基金资助:
国家重点研发计划(2022YFD1500502); 国家重点研发计划(2022YFD1500401); 国家自然科学基金(42277347); 甘肃省白银市景泰县盐碱地综合利用试点项目（第二标段）核心试验示范工程(JTYJDSDXM-SG-02); 国家现代农业产业技术体系专项(CARS-03)

Effects of Straw Returning Combined with Chemical Fertilizer on Soil Ecosystem Multifunctionality

XUAN ZePeng¹^,²(), FENG HuiYao¹^,², CHEN MeiQi²^,³, XU JiSheng², LIU MengXuan²^,⁵, ZHAO BingZi²^,⁴^,^*(), ZHANG JiaBao¹^,²^,^*()

¹ College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, Shanxi
² State Key Laboratory of Soil and Sustainable Agriculture (Institute of Soil Science, Chinese Academy of Sciences), Nanjing 211135
³ College of Geographic Sciences of Nanjing Normal University, Nanjing 210023
⁴ College of Nanjing, University of Chinese Academy of Science, Nanjing 211135
⁵ College of Forestry and Grassland & College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037

Received:2024-09-14 Accepted:2024-11-02 Published:2025-07-17 Online:2025-07-17

摘要/Abstract

摘要：

【目的】探讨秸秆还田与化肥配施对农田生态系统多功能性的影响，为秸秆与化肥的科学配施及作物生产力的提高提供理论支撑。【方法】本研究进行为期8年的冬小麦-夏玉米轮作试验（2012—2020），设置不施肥对照（NF）、单施化肥（F）、单施秸秆（S）、秸秆还田与化肥配施（FS）4个处理，测定17种常用农田生态系统服务功能指标，计算土壤生态系统多功能性指数（MFI），同时测定根际与非根际细菌和真菌群落组成，探究化肥以及秸秆还田对土壤生态系统多功能性的耦合影响及土壤性质中主要的驱动因子。【结果】F和FS较NF处理显著提高了作物产量和MFI，但S处理只增加了土壤MFI，对产量无显著影响。随机森林模型显示，N-乙酰-β-氨基葡萄糖苷酶（NAG）和全氮（TN）是影响MFI的最关键因素。施肥还显著改变了根际和非根际土壤中细菌和真菌的群落结构；与NF处理相比，F和FS处理显著增加了非根际微生物放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）和担子菌门（Basidiomycota）的相对丰度以及根际真菌担子菌门（Basidiomycota）的相对丰度。冗余分析表明，土壤有机质及其组分可溶性有机碳是影响非根际细菌和真菌群落组成的关键因素。网络分析和相关性分析发现，MFI与非根际和根际微生物网络模块相对丰度显著相关。高产组（F和FS处理）在非根际土壤中富集的物种，如Hannaella、Chaetomium等，具有较强的氨基酸、核苷酸代谢能力，对土壤生态系统多功能性具有正效应；低产组（NF与S处理）则在非根际与根际土壤中富集了不利于土壤养分固持的物种，如Solibacterales、Phycicoccus和Pleosporales。【结论】秸秆还田与化肥配施可显著提高作物产量和生态系统多功能性指数。秸秆还田配施化肥可通过增加土壤酶活性和全氮含量来增强生态系统多功能性，同时在非根际土壤中富集参与养分循环、提高酶活性和增强氨基酸和核苷酸代谢能力的特定物种（Hannaella、Chaetomium等）从而有利于维持生态系统多功能性。

关键词: 生态系统多功能性指数, 秸秆还田, 化肥, 根际微生物, 土壤酶活性, 功能预测

Abstract:

【Objective】This study aimed to explore the impact of combined application of straw returning and chemical fertilizers on the multifunctionality of farmland ecosystem, so as to provide the theoretical support for the scientific combined application of straw and chemical fertilizers and the improvement of crop productivity. 【Method】An eight-year winter wheat-summer maize rotation experiment (2012-2020) was conducted with four treatments: no fertilization control treatment (NF), single application of chemical fertilizers (F), single application of straw (S), and combined application of straw returning and chemical fertilizers (FS). 17 general indexes of farmland ecosystem service function were determined to calculate soil ecosystem multifunction index (MFI). In addition, the compositions of bacterial and fungal communities in rhizosphere and non-rhizosphere soil were determined. The coupling effects of chemical fertilizers and straw returning on the multifunctionality of soil ecosystem and the main driving factors in soil properties were explored. 【Result】Compared with NF, F and FS treatments significantly increased crop yields and MFI, but S treatment only increased soil MFI without significant effect on yield. A random forest model showed that n-acetyl-β-glucosaminase (NAG) and total nitrogen (TN) were the most important factors affecting MFI. Long-term fertilization also significantly changed the community structure of bacteria and fungi in both the rhizosphere and bulk soil compartments. Compared with the NF treatment, F and FS treatments significantly increased the relative abundance of bulk microorganisms, such as Actinobacteria, Bacteroidetes and Basidiomycota, as well as the relative abundance of rhizosphere fungi, such as Basidiomycota. The redundancy analysis showed that SOM and its component, dissolved organic carbon, were the key factors affecting the composition of bulk soil bacterial and fungal communities. Network analysis and correlation analysis further showed that MFI was significantly correlated with the relative abundances of network modules for both bulk and rhizosphere microorganisms. Moreover, the F and FS treatments with the high production level enriched bulk soil microorganisms, such as Hannaella and Chaetomium, which had strong metabolism of amino acid and nucleotide, contributing to the soil ecosystem multifunctionality. Nevertheless, for the low-yielding group (NF and S treatments), the species that were not favorable to soil nutrient retention were enriched in both the bulk and rhizosphere soil, such as Solibacterales, Phycicoccus, and Pleosporales. 【Conclusion】The combined treatment of straw incorporation and chemical fertilizer could significantly improve both crop yield and ecosystem multifunction indexes. Long-term straw returning combined with chemical fertilizer could enhance ecosystem multifunctionality by increasing soil enzyme activity and total nitrogen content. This practice also fostered the proliferation of specific species (Hannaella, Chaetomium, etc.), which played key roles in nutrient cycling, enzyme activity, and promoted amino acid and nucleotide metabolism in the bulk soil, thus contributing to the maintenance of ecosystem multifunctionality.

Key words: ecosystem multifunctionality index, straw return, chemical fertilizers, rhizosphere microbiota, soil enzyme activity, function prediction

宣泽鹏, 冯慧瑶, 陈美淇, 徐基胜, 刘梦璇, 赵炳梓, 张佳宝. 秸秆还田配施化肥对土壤生态系统多功能性的影响[J]. 中国农业科学, 2025, 58(14): 2821-2837.

XUAN ZePeng, FENG HuiYao, CHEN MeiQi, XU JiSheng, LIU MengXuan, ZHAO BingZi, ZHANG JiaBao. Effects of Straw Returning Combined with Chemical Fertilizer on Soil Ecosystem Multifunctionality[J]. Scientia Agricultura Sinica, 2025, 58(14): 2821-2837.

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参考文献 62

[1]	朱兆良, 金继运. 保障我国粮食安全的肥料问题. 植物营养与肥料学报, 2013, 19(2): 259-273.
	ZHU Z L, JIN J Y. Fertilizer use and food security in China. Journal of Plant Nutrition and Fertilizers, 2013, 19(2): 259-273. (in Chinese)
[2]	唐继伟, 林治安, 李娟, 袁亮, 徐久凯, 田昌玉, 温延臣, 赵秉强. 潮土小麦-玉米轮作体系氮肥用量阈值及土壤硝态氮年际变化. 植物营养与肥料学报, 2020, 26(12): 2246-2252.
	TANG J W, LIN Z A, LI J, YUAN L, XU J K, TIAN C Y, WEN Y C, ZHAO B Q. Optimal nitrogen rate and the down movement of soil nitrate nitrogen in wheat-maize rotation system in fluvo-aquic soil. Journal of Plant Nutrition and Fertilizers, 2020, 26(12): 2246-2252. (in Chinese)
[3]	HU Q Y, LIU T Q, DING H N, LI C F, TAN W F, YU M, LIU J, CAO C G. Effects of nitrogen fertilizer on soil microbial residues and their contribution to soil organic carbon and total nitrogen in a rice-wheat system. Applied Soil Ecology, 2023, 181: 104648.
[4]	冀建华, 吕真真, 刘淑珍, 侯红乾, 刘益仁, 刘秀梅, 李絮花, 蓝贤瑾. 长期施用化肥对南方稻田土壤酸化和盐基离子损失的影响. 中国农业科学, 2024, 57(13): 2599-2611.doi: 10.3864/j.issn.0578-1752.2024.13.008.
	JI J H, LÜ Z Z, LIU S Z, HOU H Q, LIU Y R, LIU X M, LI X H, LAN X J. Long-term application of chemical fertilizers induces soil acidification and soil exchangeable base cation loss on paddy in southern China. Scientia Agricultura Sinica, 2024, 57(13): 2599-2611. doi: 10.3864/j.issn.0578-1752.2024.13.008. (in Chinese)
[5]	孟凡乔. 中国有机农业发展: 贡献与启示. 中国生态农业学报, 2019, 27(2): 198-205.
	MENG F Q. Organic agriculture development in China: Challenges and implications. Chinese Journal of Eco-Agriculture, 2019, 27(2): 198-205. (in Chinese)
[6]	FRANCIOLI D, SCHULZ E, LENTENDU G, WUBET T, BUSCOT F, REITZ T. Mineral vs. organic amendments: Microbial community structure, activity and abundance of agriculturally relevant microbes are driven by long-term fertilization strategies. Frontiers in Microbiology, 2016, 7: 1446.
[7]	LIU E K, YAN C R, MEI X R, HE W Q, BING S H, DING L P, LIU Q, LIU S, FAN T L. Long-term effect of chemical fertilizer, straw, and manure on soil chemical and biological properties in Northwest China. Geoderma, 2010, 158(3/4): 173-180.
[8]	YIN Y F, HE X H, GAO R, MA H L, YANG Y S. Effects of rice straw and its biochar addition on soil labile carbon and soil organic carbon. Journal of Integrative Agriculture, 2014, 13(3): 491-498.
[9]	刘淑军, 李冬初, 黄晶, 曲潇林, 马常宝, 王慧颖, 于子坤, 张璐, 韩天富, 柳开楼, 申哲, 张会民. 近30年来我国小麦和玉米秸秆资源时空变化特征及还田减肥潜力. 中国农业科学, 2023, 56(16): 3140-3155. doi: 10.3864/j.issn.0578-1752.2023.16.008.
	LIU S J, LI D C, HUANG J, QU X L, MA C B, WANG H Y, YU Z K, ZHANG L, HAN T F, LIU K L, SHEN Z, ZHANG H M. Spatial-temporal variation characteristics of wheat and maize stalk resources and chemical fertilizer reduction potential of returning to farmland in recent 30 years in China. Scientia Agricultura Sinica, 2023, 56(16):3140-3155. doi: 10.3864/j.issn.0578-1752.2023.16.008. (in Chinese)
[10]	赵亚丽, 郭海斌, 薛志伟, 穆心愿, 李潮海. 耕作方式与秸秆还田对土壤微生物数量、酶活性及作物产量的影响. 应用生态学报, 2015, 26(6): 1785-1792.
	ZHAO Y L, GUO H B, XUE Z W, MU X Y, LI C H. Effects of tillage and straw returning on microorganism quantity, enzyme activities in soils and grain yield. Chinese Journal of Applied Ecology, 2015, 26(6): 1785-1792. (in Chinese)
[11]	王晶, 孙松青, 李雪, 高凤婕, 吴钦泉, 石国明, 胡凯, 耿立中, 马学文. 秸秆不同还田方式对轮作小麦-玉米产量、土壤养分及蔗糖酶活性的影响. 江苏农业科学, 2023, 51(4): 85-90.
	WANG J, SUN S Q, LI X, GAO F J, WU Q Q, SHI G M, HU K, GENG L Z, MA X W. Effects of different straw returning methods on yield, soil nutrients and sucrase activity of wheat-maize in rotation. Jiangsu Agricultural Sciences, 2023, 51(4): 85-90. (in Chinese)
[12]	DUAN Y, CHEN L, LI Y M, WANG Q Y, ZHANG C Z, MA D H, LI J Y, ZHANG J B. N, P and straw return influence the accrual of organic carbon fractions and microbial traits in a Mollisol. Geoderma, 2021, 403: 115373.
[13]	周晓果. 林下植物功能群丧失对桉树人工林土壤生态系统多功能性的影响[D]. 南宁: 广西大学, 2016.
	ZHOU X G. Effects of loss of functional groups of undergrowth plants on the versatility of soil ecosystem of eucalyptus plantation[D]. Nanning: Guangxi University, 2016. (in Chinese)
[14]	NI H W, LIU C Y, SUN B, LIANG Y T. Response of global farmland soil organic carbon to nitrogen application over time depends on soil type. Geoderma, 2022, 406: 115542.
[15]	唐继伟, 徐久凯, 温延臣, 田昌玉, 林治安, 赵秉强. 长期单施有机肥和化肥对土壤养分和小麦产量的影响. 植物营养与肥料学报, 2019, 25(11): 1827-1834.
	TANG J W, XU J K, WEN Y C, TIAN C Y, LIN Z A, ZHAO B Q. Effects of organic fertilizer and inorganic fertilizer on the wheat yields and soil nutrients under long-term fertilization. Journal of Plant Nutrition and Fertilizers, 2019, 25(11): 1827-1834. (in Chinese)
[16]	张北赢, 陈天林, 王兵. 长期施用化肥对土壤质量的影响. 中国农学通报, 2010, 26(11): 182-187.
	ZHANG B Y, CHEN T L, WANG B. Effects of long-term uses of chemical fertilizers on soil quality. Chinese Agricultural Science Bulletin, 2010, 26(11): 182-187. (in Chinese) doi: 10.11924/j.issn.1000-6850.2009-2796
[17]	WANG C L, MA Y Q, HE W H, KUZYAKOV Y, BOL R, CHEN H Q, FAN M S. Soil quality and ecosystem multifunctionality after 13-year of organic and nitrogen fertilization. The Science of the Total Environment, 2024, 931: 172789.
[18]	CHEN Q L, DING J, ZHU D, HU H W, DELGADO-BAQUERIZO M, MA Y B, HE J Z, ZHU Y G. Rare microbial taxa as the major drivers of ecosystem multifunctionality in long-term fertilized soils. Soil Biology and Biochemistry, 2020, 141: 107686.
[19]	陈美淇, 马垒, 赵炳梓, 范树印, 谭钧, 鞠振山, 朱锦尉, 徐国华, 王淑媛, 徐基胜, 张佳宝. 木本泥炭对红黄壤性水田土壤有机质提升和细菌群落组成的影响. 土壤, 2020, 52(2): 279-286.
	CHEN M Q, MA L, ZHAO B Z, FAN S Y, TAN J, JU Z S, ZHU J W, XU G H, WANG S Y, XU J S, ZHANG J B. Effects of woody peat on quick improvement of soil organic matter and bacterial community composition in newly reclaimed red-yellow paddy soils. Soils, 2020, 52(2): 279-286. (in Chinese)
[20]	梁路, 张卫杰, 徐博涵, 庄秋丽, 姜秀芳, 黄玉波. 有机无机肥配施影响土壤肥力与土壤环境的研究进展. 河南农业科学, 2022, 51(3): 1-11.
	LIANG L, ZHANG W J, XU B H, ZHUANG Q L, JIANG X F, HUANG Y B. Research progress on effects of combined application of organic and inorganic fertilizers on soil fertility and soil environment. Journal of Henan Agricultural Sciences, 2022, 51(3): 1-11. (in Chinese) doi: 10.15933/j.cnki.1004?3268.2022.03.001
[21]	马垒, 李燕, 魏建林, 李子双, 周晓琳, 郑福丽, 吴小宾, 王利, 刘兆辉, 谭德水. 长期秸秆还田对潮土真菌群落、酶活性和小麦产量的影响. 环境科学, 2022, 43(10): 4755-4764.
	MA L, LI Y, WEI J L, LI Z S, ZHOU X L, ZHENG F L, WU X B, WANG L, LIU Z H, TAN D S. Effects of long-term straw returning on fungal community, enzyme activity and wheat yield in fluvo-aquic soil. Environmental Science, 2022, 43(10): 4755-4764. (in Chinese)
[22]	XU H D, ZHU B, WEI X M, YU M K, CHENG X R. Root functional traits mediate rhizosphere soil carbon stability in a subtropical forest. Soil Biology and Biochemistry, 2021, 162: 108431.
[23]	HU W, ZHANG Y P, RONG X M, ZHOU X, FEI J C, PENG J W, LUO G W. Biochar and organic fertilizer applications enhance soil functional microbial abundance and agroecosystem multifunctionality. Biochar, 2024, 6(1): 3.
[24]	鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科学出版社, 1999: 296-338.
	LU R K. Methods of Soil Agricultural Chemistry Analysis. Beijing: Chinese Agricultural Science and Technology Press, 1999: 296-338. (in Chinese)
[25]	JONES D, WILLETT V. Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil. Soil Biology and Biochemistry, 2005, 38(5): 991-999.
[26]	韩紫璇, 房静静, 武雪萍, 姜宇, 宋霄君, 刘晓彤. 长期秸秆配施化肥下土壤团聚体碳氮分布、微生物量与小麦产量的协同效应. 中国农业科学, 2023, 56(8): 1503-1514. doi: 10.3864/j.issn.0578-1752.2023.08.007.
	HAN Z X, FANG J J, WU X P, JIANG Y, SONG X J, LIU X T. Synergistic effects of organic carbon and nitrogen content in water-stable aggregates as well as microbial biomass on crop yield under long-term straw combined chemical fertilizers application. Scientia Agricultura Sinica, 2023, 56(8): 1503-1514. doi: 10.3864/j.issn.0578-1752.2023.08.007. (in Chinese)
[27]	VERCHOT L, BORELLI T. Application of-nitrophenol (NP) enzyme assays in degraded tropical soils. Soil Biology and Biochemistry, 2005, 37(4): 625-633.
[28]	关松荫. 土壤酶及其研究法. 北京: 农业出版社, 1986: 294-338.
	GUAN S Y. Soil Enzyme and Its Research Method. Beijing: Agricultural Press, 1986: 294-338. (in Chinese)
[29]	MAESTRE F T, QUERO J L, GOTELLI N J, ESCUDERO A, OCHOA V, DELGADO-BAQUERIZO M, GARCÍA-GÓMEZ M, BOWKER M A, SOLIVERES S, ESCOLAR C, et al. Plant species richness and ecosystem multifunctionality in global drylands. Science, 2012, 335(6065): 214-218. doi: 10.1126/science.1215442 pmid: 22246775
[30]	熊定鹏, 赵广帅, 武建双, 石培礼, 张宪洲. 羌塘高寒草地物种多样性与生态系统多功能关系格局. 生态学报, 2016, 36(11): 3362-3371.
	XIONG D P, ZHAO G S, WU J S, SHI P L, ZHANG X Z. The relationship between species diversity and ecosystem multifunctionality in alpine grasslands on the Tibetan Changtang Plateau. Acta Ecologica Sinica, 2016, 36(11): 3362-3371. (in Chinese)
[31]	白震, 张明, 宋斗妍, 张旭东. 不同施肥对农田黑土微生物群落的影响. 生态学报, 2008, 28(7): 3244-3253.
	BAI Z, ZHANG M, SONG D Y, ZHANG X D. Effect of different fertilizaiton on microbial community in an arable mollisol. Acta Ecologica Sinica, 2008, 28(7): 3244-3253. (in Chinese)
[32]	冯海萍, 陈卓, 杨虎. 微生物菌剂对连作芹菜根际土壤真菌群落多样性与结构的影响. 干旱地区农业研究, 2024, 42(2): 53-61, 70.
	FENG H P, CHEN Z, YANG H. Effects of biocontrol agents on fungal community diversity and structure in rhizosphere soil of continuous cropping celery. Agricultural Research in the Arid Areas, 2024, 42(2): 53-61, 70. (in Chinese)
[33]	ROGNES T, FLOURI T, NICHOLS B, QUINCE C, MAHÉ F. VSEARCH: A versatile open source tool for metagenomics. PeerJ, 2016, 4: e2584.
[34]	赵自超, 韩笑, 石岳峰, 吴文良, 孟凡乔. 硝化和脲酶抑制剂对华北冬小麦-夏玉米轮作固碳减排效果评价. 农业工程学报, 2016, 32(6): 254-262.
	ZHAO Z C, HAN X, SHI Y F, WU W L, MENG F Q. Effect of nitrification and urease inhibitor on carbon sequestration and greenhouse gas emissions in winter wheat and summer maize rotation system in North China. Transactions of the Chinese Society of Agricultural Engineering, 2016, 32(6): 254-262. (in Chinese)
[35]	LI K L, ZHANG H Y, LI X L, WANG C, ZHANG J L, JIANG R F, FENG G, LIU X J, ZUO Y M, YUAN H M, ZHANG C C, GAI J P, TIAN J. Field management practices drive ecosystem multifunctionality in a smallholder-dominated agricultural system. Agriculture, Ecosystems & Environment, 2021, 313: 107389.
[36]	WU G, LING J, ZHAO D Q, LIU Z X, XU Y P, KUZYAKOV Y, MARSDEN K, WEN Y, ZHOU S L. Straw return counteracts the negative effects of warming on microbial community and soil multifunctionality. Agriculture, Ecosystems & Environment, 2023, 352: 108508.
[37]	甄丽莎, 谷洁, 高华, 秦清军, 陈强龙. 秸秆还田与施肥对土壤酶活性和作物产量的影响. 西北植物学报, 2012, 32(9): 1811-1818.
	ZHEN L S, GU J, GAO H, QIN Q J, CHEN Q L. Effect of straws, manure and chemical fertilizer on soil properties and crop yields. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(9): 1811-1818. (in Chinese)
[38]	陈素英, 张喜英, 孙宏勇, 邵立威. 华北平原秸秆覆盖冬小麦减产原因分析. 中国生态农业学报, 2013, 21(5): 519-525.
	CHEN S Y, ZHANG X Y, SUN H Y, SHAO L W. Analysis on the causes of yield reduction of winter wheat under straw mulch in North China Plain. Chinese Journal of Eco-Agriculture, 2013, 21(5): 519-525. (in Chinese)
[39]	TANG H M, XIAO X P, TANG W G, LIN Y C, WANG K, YANG G L. Effects of winter cover crops residue returning on soil enzyme activities and soil microbial community in double-cropping rice fields. PLoS ONE, 2014, 9(6): e100443.
[40]	范淼珍, 尹昌, 范分良, 宋阿琳, 王伯仁, 李冬初, 梁永超. 长期不同施肥对红壤碳、氮、磷循环相关酶活性的影响. 应用生态学报, 2015, 26(3): 833-838.
	FAN M Z, YIN C, FAN F L, SONG A L, WANG B R, LI D C, LIANG Y C. Effects of different long-term fertilization on the activities of enzymes related to carbon, nitrogen, and phosphorus cycles in a red soil. Chinese Journal of Applied Ecology, 2015, 26(3): 833-838. (in Chinese)
[41]	ZHAO S C, LI K J, ZHOU W, QIU S J, HUANG S W, HE P. Changes in soil microbial community, enzyme activities and organic matter fractions under long-term straw return in north-central China. Agriculture, Ecosystems & Environment, 2016, 216: 82-88.
[42]	林先贵, 冯有智. 潮土农田微生物研究进展. 中国生态农业学报, 2016, 24(4): 416-434.
	LIN X G, FENG Y Z. Research progresses of farmland microorganisms in fluvo-aquic soil of China. Chinese Journal of Eco-Agriculture, 2016, 24(4): 416-434. (in Chinese)
[43]	郭伟, 周云鹏, 陈美淇, 李丹丹, 王青霞, 周谈坛, 赵炳梓. 秸秆与有机无机肥配施对潮土关键微生物及小麦产量的影响. 土壤学报, 2024, 61(4): 1134-1146.
	GUO W, ZHOU Y P, CHEN M Q, LI D D, WANG Q X, ZHOU T T, ZHAO B Z. Effects of combined application of straw and organic- inorganic fertilizers on key microorganisms and wheat yield in fluvo-aquic soil. Acta Pedologica Sinica, 2024, 61(4): 1134-1146. (in Chinese)
[44]	练金山, 王慧颖, 徐明岗, 魏文良, 段英华, 刘树堂. 长期施用有机肥潮土细菌的多样性及功能预测. 植物营养与肥料学报, 2021, 27(12): 2073-2082.
	LIAN J S, WANG H Y, XU M G, WEI W L, DUAN Y H, LIU S T. Bacterial diversity and functional prediction of long-term application of organic fertilizer in tidal soil. Journal of Plant Nutrition and Fertilizers, 2021, 27(12): 2073-2082. (in Chinese)
[45]	周晶. 长期施氮对东北黑土微生物及主要氮循环菌群的影响[D]. 北京: 中国农业大学, 2017.
	ZHOU J. Effects of long-term nitrogen application on microbes and main nitrogen circulating flora in black soil of Northeast China[D]. Beijing: China Agricultural University, 2017. (in Chinese)
[46]	KLAUBAUF S, INSELSBACHER E, ZECHMEISTER-BOLTENSTERN S, WANEK W, GOTTSBERGER R, STRAUSS J, GORFER M. Molecular diversity of fungal communities in agricultural soils from Lower Austria. Fungal Diversity, 2010, 44(1): 65-75. pmid: 23794962
[47]	WANG J T, ZHENG Y M, HU H W, ZHANG L M, LI J, HE J Z. Soil pH determines the alpha diversity but not beta diversity of soil fungal community along altitude in a typical Tibetan forest ecosystem. Journal of Soils and Sediments, 2015, 15(5): 1224-1232.
[48]	MA A Z, ZHUANG X L, WU J M, CUI M M, LÜ D, LIU C Z, ZHUANG G Q. Ascomycota members dominate fungal communities during straw residue decomposition in arable soil. PLoS ONE, 2013, 8(6): e66146.
[49]	罗嘉润, 宋文杰, 刘伟, 张大弘, 江勋, 卢碧林. 秸秆还田配施氮肥早期对水稻生长、土壤性质及微生物多样性的影响. 农业生物技术学报, 2023, 31(10): 2019-2034.
	LUO J R, SONG W J, JIU W, ZHANG D H, JIANG X, LU B L. Effects of straw returning combined with nitrogen fertilizer on rice growth, soil properties and microbial diversity in early stage. Journal of Agricultural Biotechnology, 2023, 31(10): 2019-2034. (in Chinese)
[50]	赵金花, 陈林, 段衍, 张丛志, 马东豪, 张佳宝. 秸秆还田配合化肥减施对潮土作物产量及土壤肥力的影响. 土壤学报, 2023, 60(1): 189-200.
	ZHAO J H, CHEN L, DUAN Y, ZHANG C Z, MA D H, ZHANG J B. Effects of straw returning instead of chemical fertilizer on crop yield and soil fertility in fluvo-aquic soil. Acta Pedologica Sinica, 2023, 60(1): 189-200. (in Chinese)
[51]	SONG Y, LI X N, YAO S, YANG X L, JIANG X. Correlations between soil metabolomics and bacterial community structures in the pepper rhizosphere under plastic greenhouse cultivation. The Science of the Total Environment, 2020, 728: 138439.
[52]	GE Z, LI S Y, BOL R, ZHU P, PENG C, AN T T, CHENG N, LIU X, LI T Y, XU Z Q, WANG J K. Differential long-term fertilization alters residue-derived labile organic carbon fractions and microbial community during straw residue decomposition. Soil and Tillage Research, 2021, 213: 105120.
[53]	李月, 张水清, 韩燕来, 李世莹, 王祎, 李慧, 李芳, 谭金芳. 长期秸秆还田与施肥对潮土酶活性和真菌群落的影响. 生态环境学报, 2020, 29(7): 1359-1366. doi: 10.16258/j.cnki.1674-5906.2020.07.010
	LI Y, ZHANG S Q, HAN Y L, LI S Y, WANG Y, LI H, LI F, TAN J F. Effects on fluvo-aquic soil enzyme activity and fungal community of long-term straw returning and fertilization. Ecology and Environmental Sciences 2020, 29(7): 1359-1366. (in Chinese)
[54]	KOECHLI C, CAMPBELL A N, PEPE-RANNEY C, BUCKLEY D H. Assessing fungal contributions to cellulose degradation in soil by using high-throughput stable isotope probing. Soil Biology and Biochemistry, 2019, 130: 150-158.
[55]	LINDAHL B D, FINLAY R D. Activities of chitinolytic enzymes during primary and secondary colonization of wood by basidiomycetous fungi. The New Phytologist, 2006, 169(2): 389-397.
[56]	VAN DER WAL A, KLEIN GUNNEWIEK P, DE HOLLANDER M, DE BOER W. Fungal diversity and potential tree pathogens in decaying logs and stumps. Forest Ecology and Management, 2017, 406: 266-273.
[57]	LIN Y X, YE G P, KUZYAKOV Y, LIU D Y, FAN J B, DING W X. Long-term manure application increases soil organic matter and aggregation, and alters microbial community structure and keystone taxa. Soil Biology and Biochemistry, 2019, 134: 187-196.
[58]	SULEIMAN A K A, LOURENÇO K S, PITOMBO L M, MENDES L W, ROESCH L F W, PIJL A, CARMO J B, CANTARELLA H, KURAMAE E E. Recycling organic residues in agriculture impacts soil-borne microbial community structure, function and N₂O emissions. The Science of the Total Environment, 2018, 631/632: 1089-1099.
[59]	CHEN Q Y, LIU Z J, ZHOU J B, XU X P, ZHU Y J. Long-term straw mulching with nitrogen fertilization increases nutrient and microbial determinants of soil quality in a maize-wheat rotation on China’s Loess Plateau. Science of the Total Environment, 2021, 775: 145930.
[60]	LEE S D. Phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed. International Journal of Systematic and Evolutionary Microbiology, 2006, 56(10): 2369-2373.
[61]	ZHANG Q W, YANG Z L, ZHANG H, YI J. Recovery efficiency and loss of ¹⁵N-labelled urea in a rice-soil system in the upper reaches of the Yellow River Basin. Agriculture, Ecosystems & Environment, 2012, 158: 118-126.
[62]	WANG Q F, MA M C, JIANG X, ZHOU B K, GUAN D W, CAO F M, CHEN S F, LI J. Long-term N fertilization altered ¹³C-labeled fungal community composition but not diversity in wheat rhizosphere of Chinese black soil. Soil Biology and Biochemistry, 2019, 135: 117-126.

土壤性质 Soil property	NF	F	S	FS
pH	8.53±0.04a	8.26±0.03c	8.48±0.02b	8.19±0.04d
有机质 Soil organic matter, SOM (g·kg^-1)	9.90±1.02c	12.95±0.39b	13.20±0.61b	22.11±1.33a
全氮 Total nitrogen, TN (g·kg^-1)	0.62±0.04d	0.84±0.06b	0.74±0.01c	1.05±0.06a
全磷 Total Phosphorus, TP (g·kg^-1)	0.95±0.06b	1.37±0.14a	0.91±0.05b	1.31±0.09a
全钾 Total potassium, TK (g·kg^-1)	15.7±0.59a	16.36±0.58a	15.08±0.35a	16.43±0.68a
有效磷 Available phosphorus, AP (mg·kg^-1)	2.47±0.88b	9.08±1.08a	2.68±1.10b	9.92±1.93a
速效钾 Available potassium, AK (mg·kg^-1)	52.68±2.06d	100.39±8.49b	71.98±6.19c	159.57±11.18a
硝态氮 Nitrate nitrogen, NO₃^--N (mg·kg^-1)	6.90±0.85c	8.32±0.92b	9.88±0.76b	13.18±2.68a
微生物生物量碳 Microbial biomass carbon, MBC (mg·kg^-1)	185.12±31.16d	352.17±34.21b	264.22±36.45c	454.91±68.84a
微生物生物量氮 Microbial biomass nitrogen, MBN (mg·kg^-1)	27.84±4.22c	53.15±4.14a	27.84±2.00c	47.03±5.43b
可溶性有机碳 Dissolved organic carbon, DOC (mg·kg^-1)	30.16±1.92b	34.41±3.97b	32.89±1.58b	44.25±3.50a
可溶性有机氮 Dissolved organic nitrogen, DON (mg·kg^-1)	5.19±0.73b	6.44±2.63b	8.27±0.70b	9.64±1.08a

酶活性 Enzyme activity	NF	F	S	FS
β-葡萄糖苷酶 β-glucosidase, BG (mg·g^-1·h^-1)	166.2±12.57d	280.5±12.1b	215.83±17.31c	479.75±67.85a
N-乙酰-β-氨基葡萄糖苷酶 β-1,4-N-acetylglucosaminidase, NAG (mg·kg^-1·h^-1)	35.65±2.51d	79.89±4.67b	52.50±9.31c	130.86±17.07a
脲酶 Urease, UR (mg·kg^-1·h^-1)	130.86±17.07a	1.63±0.21b	2.17±0.12a	1.23±0.29c
碱性磷酸酶 Alkaline phosphatase, AKP (mg·g^-1·h^-1)	901.09±69.33d	1604.89±186.31b	1078.93±93.18c	1979.98±282.57a
转化酶 Invertase, INV (mg·g^-1·h^-1)	1362.49±115.25d	2343.29±247.09b	1751.53±92.22c	3682.54±467.48a

	非根际土壤模块相对丰度 Relative abundances of module in bulk soil				根际土壤模块相对丰度 Relative abundances of module in rhizosphere soil
	Module1	Module2	Module3		Module1	Module2	Module3
生态多功能指数MFI	-0.133	0.523**	0.406		-0.436	-0.551**	-0.338

秸秆还田配施化肥对土壤生态系统多功能性的影响

Effects of Straw Returning Combined with Chemical Fertilizer on Soil Ecosystem Multifunctionality

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