关键词: 葡萄, 花发育, 亚细胞定位, 表达分析, VvAG, VvAP3, VvFLC, VvFUL

Abstract:

【Objective】The aim of this study was to isolate the open reading frame sequence of Vitis vinifera AGAMOUS (VvAG), Vitis vinifera APETALA 3 (VvAP3), Vitis vinifera FLOWERING LOCUS C (VvFLC), Vitis vinifera FRUITFUL (VvFUL), Vitis vinifera FLOWERING LOCUS T (VvFT), Vitis vinifera APETALA2 (VvAP2) and Vitis vinifera SUPPRESSOR OF OVER EXPRESSION OF CO 1 (VvSOC1) from ‘Xiangyue’, one of the most popular table grape cultivars, and for some preliminary study on their functions. 【Method】 Specific primers RT-PCR method was used to clone genes, and semi-quantitative PCR was used to analyze the expression of genes in different organs and tissues. Recombinant plasmid was introduced into onion epidermal cells by the particle bombardment method with a PDS1000/He. Transformed cells were incubated for 24 h at 25℃ in the dark and green fluorescence was monitored under a Laser scanning confocal microscope. 【Result】The expression results of the genes in different tissues showed that they were expressed ubiquitously in all the organs and tissues, but the expression levels were some different. VvFT, VvFUL and VvAP3 were expressed highest in young fruit, VvAG and VvAP2 highest in flower, and VvSOC1 and VvFLC highest in young leaves. VvSOC1, VvFT, VvFLC and VvAP2 combined with GFP were only located in nucleus of onion epidermal cell, which showed typical characteristics of transcription factor; however, VvAG, VvFUL and VvAP3 combined with GFP were located in both the plasma membrane and nucleus. 【Conclusion】All these genes were involved in the developments both of reproductive and vegetative organs, and showed the nucleus location phenomena by combining with GFP except that VvAG, VvFUL, and VvAP3 showed signal at plasma membrane.

Key words: VvSOC1, VvAP2, VvFT, grapevine, flower development, sub-cellular localization, expression analysis, VvAG, VvAP3, VvFLC, VvFUL