关键词: 耐冬山茶, AFLP标记, 遗传多样性, 亲缘关系

Abstract:

【Objective】The genetic diversity of Camellia Japonica (Naidong) cultivar accessions was analyzed at molecular level,and the helpful information for cultivar classification was obtained.【Method】The genetic diversity of 16 cultivars and 2 mutation types of Camellia Japonica (Naidong) with 5 cultivars of C. japonica from south of China, 2 cultivars of C. reticulata was analyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoRⅠ/Mse 1 primer combinations. 【Result】 In the genomes of the 30 tested samples,a total of 1 062 spectral bands amplified from the 8 pairs of primer combinations were selected from AFLP analysis and 987 bands appeared to be polymorphic (92.9%). The data were used to calculate the Nei’s genetic distance and to construct a dendrogram based on UPGMA cluster analysis. 【Conclusion】 It showed that the cultivars of Camellia japonica (Naidong) have a remote genetic relationship compared with the cultivars of Camellia from south of China,and cultivars of Camellia japonica (Naidong) can be divided into 6 groups,which indicated that Camellia japonica (Naidong) have rich genetic variability.

Key words: Camellia japonica (Naidong), AFLP marker, genetic diversity, genetic relationship