中国农业科学 ›› 2007, Vol. 40 ›› Issue (4): 677-684 .

• 作物遗传育种·种质资源 • 上一篇    下一篇

甘蓝型油菜含油量及皮壳率的QTL分析

金梦阳,李加纳,付福友,张正圣,张学昆,刘列钊   

  1. 西南大学农学与生命科学学院
  • 收稿日期:2006-03-22 修回日期:1900-01-01 出版日期:2007-04-10 发布日期:2007-04-10
  • 通讯作者: 刘列钊

QTL Analysis of Oil Content and Hull Content in Brassica napus L.

  

  1. 西南大学农学与生命科学学院
  • Received:2006-03-22 Revised:1900-01-01 Online:2007-04-10 Published:2007-04-10

摘要: 【目的】通过构建甘蓝型油菜遗传连锁图谱,对含油量及皮壳率进行QTL分析。【方法】以黄籽亲本GH06和黑籽亲本P174杂交得到的F2:6家系的188个株系为作图群体,利用SRAP、SSR、AFLP及TRAP四种标记构建遗传连锁图谱,在此基础上采用复合区间作图法(CIM)对含油量及皮壳率两个性状进行QTL分析。【结果】图谱包含19个连锁群、300个标记位点,总长为1 248.5 cM。共得到7个与含油量相关的QTL,单位点遗传贡献率在3.73%~10.46%之间;4个与皮壳率相关的QTL,单位点遗传贡献率在4.89%~6.84%之间。【结论】黄籽油菜具有高含油量的优势;皮壳率对含油量有显著影响;SRAP标记具有较好的检测QTL位点的能力。

关键词: 甘蓝型油菜, 遗传图谱, 分子标记, 数量性状位点, 含油量, 皮壳率

Abstract: Abstract:【Objective】Constructing the linkage map of Brassica napus L. and mapping the QTL of oil content and hull content. 【Method】F2:6 RIL population with 188 lines, crossed by GH06 as female parent and Youyan 2 as male parent,were used as mapping Population. The SRAP、SSR、AFLP and TRAP markers were used to construct the linkage map, and the composite interval mapping (CIM) was used to identify quantitative trait loci associated with oil content and hull content.【Result】300 markers were integrated into 19 groups with software JoinMap3.0. It covered 1248.5cM with a mean marker interval of 4.16cM. Eight QTL were found for oil content with the phenotypic variance explained of 5.95%~9.47%; Two for hull content with the phenotypic variance explained of 6.91%and 6.05%respectively. 【Conclusion】It was confirmed that SRAP marker was a good marker system for the construction of linkage map. The hull content has a great effect on the oil content.

Key words: Brassica napus L, Genetic map, Molecular markers, QTL, Oil content, Hull content