中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2274-2280 .

• 园艺 • 上一篇    下一篇

设施黄瓜连作和轮作中土壤微生物群落多样性的变化及其与产量品质的关系

吴凤芝,王学征   

  1. 东北农业大学园艺学院
  • 收稿日期:2006-06-23 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10

Study of Relativity of Soil Microbial Diversity and Cucumber Yield, Quality in Protected Cultivation

  

  1. 东北农业大学园艺学院
  • Received:2006-06-23 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】研究设施黄瓜连作和轮作中,土壤微生物多样性的变化及其对产量品质的影响。【方法】利用RAPD技术研究了设施黄瓜连作和轮作对土壤微生物群落DNA序列多样性及产量品质的影响。【结果】连作黄瓜2年和7年的土壤微生物群落多样性指数、丰富度及其均匀度指数均随着黄瓜种植年限的增加而降低,黄瓜产量显著下降。而对于15、18和21年3种轮作种植的土壤,尽管其种植年限较长,但其微生物群落DNA序列多样性及均匀度均高于7年黄瓜连作的土壤,而且黄瓜VC含量均高于连作土壤。【结论】设施栽培条件下,轮作有利于土壤微生物群落的多样性和稳定性的提高,有利于土壤生态环境的改善和黄瓜产量品质的形成。

关键词: RAPD, 多样性指数, 均匀度指数, 产量, 品质

Abstract: Abstract: 【Objective】 To study the effect of cucumber planting years and cultivated modes on DNA sequence diversity of microbial community and relativity with cucumber yield and quality. 【Method】RAPD techniques were adopted to study the effect of cucumber planting years and cultivated modes on DNA sequence diversity of microbial community and relativity with cucumber yield and quality.【Result】 The results showed that the diversity index, richness and evenness index of microbial community which was in soil of continuous cropping cucumber for 2 years and 7 years decreased with increasing of planting years, and cucumber yield dropped evidently. However, in soil of crop rotation for 15, 18 and 21 years, though the planting years were longer, DNA sequence diversity and evenness of microbial community were both higher than those in soil of continuous cropping cucumber for 7 years. It showed that the rotation of crops is propitious to DNA sequence diversity and stability of microbial community, and also the vitamin C content of cucumber of rotation was higher than that of continuous cropping soil cultivation. DNA sequence diversity index of soil microbial community was positively correlated with cucumber yield and quality evidently.

Key words: RAPD, Diversity Index, Evenness Index, Yield, Relativity