中国农业科学 ›› 2006, Vol. 39 ›› Issue (11): 2250-2254 .

• 植物保护 • 上一篇    下一篇

与黄瓜抗黑星病基因连锁的分子标记研究

张桂华,韩毅科,孙小红,李淑菊,魏爱民,杜胜利   

  1. 南开大学生命科学学院
  • 收稿日期:2006-08-08 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10
  • 通讯作者: 杜胜利

Molecular Markers Linked to High Temperature Resistance QTLs in Cucumber

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  1. 南开大学生命科学学院
  • Received:2006-08-08 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

摘要: 【目的】筛选与黄瓜抗黑星病基因连锁的分子标记,探讨黄瓜抗病材料鉴定和选择的分子方法。【方法】以黄瓜抗黑星病和感黑星病亲本组合(Q6×Q12)的F2分离群体为试材,采用BSA法和AFLP技术筛选与黄瓜抗黑星病基因连锁的分子标记。【结果】AFLP引物组合E20M64在抗病池和感病池间约130 bp处表现多态性。经F2单株分析,在抗病个体中扩增出了约为130 bp的特异片段,而感病个体无此条带。该特异标记与黑星病抗性基因紧密连锁,遗传距离为4.83 cM。测序结果显示,目标片段的大小为125 bp,并将该标记转换为了SCAR标记。【结论】提供了黄瓜黑星病抗性鉴定的分子手段,可用于分子标记辅助育种。

关键词: 黄瓜, 黑星病, 分子标记, AFLP, SCAR

Abstract: 【OBJECTIVE】High temperature resistance trait is very important in cucumber production in summer. In order to higher the efficiency of breeding high temperature resistance cucumber lines, molecular markers associated to this trait was analyzed in this research;【METHOD】Two inbred lines, 863-7 (heat-tolerant) and 863-6 (heat-susceptible), were used to construct F2 population. Bulked segregant analysis and selective genotyping were used to screen the candidate SSR and SRAP markers. The screened markers were analyzed in the F2 population;【RESULTS】Sixteen candidate markers, which possibly associated to the high temperature resistance, were gotten. After analyzed with F2 population, 10 markers including 1 SSR marker and 9 SRAP markers were found to be associated with high temperature resistance QTL, each accounted for 6%~17% of phenotypic variation. Mapmaker analysis revealed that these markers represented 3 QTLs accounting for 32.3% of total phenotypic variation;【CONCLUSION】Molecular markers linked to high temperature resistance QTL were selected in this research. It will lay a foundation for high temperature resistance line breeding in cucumber.