中国农业科学 ›› 2005, Vol. 38 ›› Issue (07): 1369-1372 .

• 植物保护 • 上一篇    下一篇

大豆对斜纹夜蛾抗性的遗传分析及相关QTL的定位

刘华,王慧,李群,徐鹏,盖钧镒,喻德跃   

  1. 南京农业大学大豆研究所,国家大豆改良中心,作物遗传与种质创新国家重点实验室
  • 收稿日期:2005-03-31 修回日期:1900-01-01 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 喻德跃 nauwh3

Inheritance Analysis and Mapping QTLs Related to Cotton Worm Resistance in Soybeans

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  1. 南京农业大学大豆研究所,国家大豆改良中心,作物遗传与种质创新国家重点实验室
  • Received:2005-03-31 Revised:1900-01-01 Online:2005-07-10 Published:2005-07-10

摘要: 以大豆组合皖82-178×通山薄皮黄豆甲衍生的重组自交系群体(RIL)为材料,以斜纹夜蛾幼虫重为抗性鉴定指标,应用主基因+多基因的混合遗传模型对大豆抗虫性进行遗传分析。结果表明,该群体对斜纹夜蛾的抗性遗传符合两对主基因+多基因的遗传模型,主基因的遗传率为89.85%。以该群体所构建的遗传连锁图谱为基础,利用软件Cartgrapher(V.2.0)采用复合区间作图法检测到2个与抗虫有关的QTL,分别位于wt-11和wt-12连锁群上,其在对应连锁群的端距离分别为5.51cM、11.51cM,加性效应估计值分别为-0.0619、-0.0419,对性状变异的解释率分别为17.22%和8.60%。

关键词: 大豆抗虫, 斜纹夜蛾, 遗传分析, QTL定位

Abstract: This study employed the soybean Recombinant Inbred Lines(RIL) which involved 133 lines from Wan82-178×Tongshanbopihuangdoujia as experimental materials. Larval weight of cotton worm (Prodenia litura (L.) Fabricius) was examined in as index of resistance. The inheritance of soybean resistance to cotton worm was estimated by major gene plus polygene mixed model, the result indicated that resistance was controlled by two major genes plus polygene, and the heritability value of major genes is 89.85%. Based on the linkage map constructed with SSR markers of this RIL population, two QTLs related to the trait were mapped on linkage group wt-11 and wt-12, and the position were 5.51cM and 11.51cM, respectively. The QTLs’ additive effects were –0.0619 and –0.0419 and explained 17.22% and 8.60% of the total variation of the larval weight, respectively.

Key words: Soybean Insect Resistance, Cotton worm, Inheritance Analysis, QTLs mapping