中国农业科学 ›› 2005, Vol. 38 ›› Issue (07): 1364-1368 .

• 植物保护 • 上一篇    下一篇

小麦抗叶锈病基因Lr45的AFLP分子标记

张娜,刘大群,杨文香,闫红飞,褚 栋,孟庆芳,张 汀   

  1. 河北农业大学植物病理系
  • 收稿日期:2004-12-01 修回日期:1900-01-01 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 刘大群

Molecular Markers of Wheat Leaf Rust Resistance Gene Lr45 Based on AFLP

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  1. 河北农业大学植物病理系
  • Received:2004-12-01 Revised:1900-01-01 Online:2005-07-10 Published:2005-07-10

摘要: 利用AFLP技术对小麦抗叶锈基因Lr45进行了标记,从60对AFLP引物中筛选出2对在亲本及TcLr45×Thatcher F2抗感群体间揭示多态性的引物P-AGG/M-GAG和P-ACA/M-GGT。其扩增片段为261 bp和105 bp,2个标记与Lr45的遗传距离分别为0.6 cM和1.3 cM。测序比较261 bp片段与大麦属Vulgare HotrI基因部分序列同源性达86%,105 bp片段与一粒小麦磷脂酰丝氨酸脱羧酶基因部分序列同源性高达96%。2个测序片段均包含开放阅读框(ORF)序列。

关键词: 小麦叶锈病, AFLP, Lr45, 基因定位

Abstract: AFLP technology was employed to isolate wheat leaf rust resistance gene Lr45. Sixty primer pairs were screened, of which two primer pairs, P-AGG/M-GAG and P-ACA/M-GGT, displayed polymorphsim in the population of TcLr45, Thatcher, and their F2 generation. Two markers were found tightly linked to Lr45. One links to the Lr45 locus with a distance of 0.6 cM, and the other with a distance of 1.3 cM. The fragement of 261 bp was 86 percent the same as a part of the original sequence of Vulgare HortI gene, and the 105 bp was 96 percent compared with a part of the triticum monococcum phosphatidylserine decarboxylase gene. Both of them included an open reading frame (ORF).

Key words: Wheat leaf rust, AFLP, Lr45, Gene localization