中国农业科学 ›› 2020, Vol. 53 ›› Issue (11): 2149-2160.doi: 10.3864/j.issn.0578-1752.2020.11.002
梁慧珍1,2,许兰杰1,董薇1,余永亮1,杨红旗1,谭政委1,李磊1,刘新梅1
收稿日期:
2019-09-26
接受日期:
2019-12-03
出版日期:
2020-06-01
发布日期:
2020-06-09
作者简介:
梁慧珍,Tel:0371-65751589;E-mail: lhzh66666@163.com
基金资助:
LIANG HuiZhen1,2,XU LanJie1,DONG Wei1,YU YongLiang1,YANG HongQi1,TAN ZhengWei1,LI Lei1,LIU XinMei1
Received:
2019-09-26
Accepted:
2019-12-03
Online:
2020-06-01
Published:
2020-06-09
摘要:
【目的】通过对大豆γ-生育酚进行混合遗传和QTL定位分析,了解其遗传机制,定位其主效QTL,为高γ-生育酚含量大豆品种的选育奠定基础。【方法】以栽培大豆晋豆23为母本,以山西农家品种大豆灰布支黑豆为父本杂交衍生的重组自交系作为供试群体构建遗传图谱。图谱全长2 047.6 cM,平均图距8.8 cM,包括227个SSR标记,232个标记位点。重组自交系试验群体及亲本材料分别于2011年、2012年和2015年夏季在河南省农业科学院原阳试验基地种植,冬季在海南省三亚南繁试验基地种植。田间试验采取随机区组设计,2次重复。从6个环境中每个家系选取15.00 g籽粒饱满,大小一致的大豆种子,利用高效液相色谱法定性、定量测定样品中的γ-生育酚含量。采用主基因+多基因混合遗传分离分析法,对大豆γ-生育酚含量进行混合遗传分析;采用WinQTLCart 2.5复合区间作图法,对大豆γ-生育酚含量进行QTL定位分析。【结果】主基因+多基因混合遗传分析表明,γ-生育酚受2对重叠作用主基因×加性多基因控制。遗传基因分布在双亲中。三亚试验数据检测出2对主基因间上位性效应值为0.4010—0.5169和多基因的加性效应值为0.1797—0.2146,主基因遗传率为11.27%—13.05%,多基因遗传率为82.51%—86.55%,多基因效应大于主基因效应。原阳试验数据检测到2对主基因间上位性效应值为0.9646—1.8455,主基因遗传率为39.51%—58.96%,没有检测出多基因效应。采用WinQTLCart 2.5复合区间作图(CIM)共检测到9个影响γ-生育酚的QTL,分布于A1(Chr.5)、A2(Chr.8)、C1(Chr.4)、K(Chr.9)、M(Chr.7)和G(Chr.8)6条染色体中,单个QTL的贡献率为7.29%—29.55%。qγ-G-1同时在2011年原阳、2012年三亚、2015年三亚3个环境下检测到,且均定位在G(Chr.18)染色体Satt275—Satt038标记区间0.01 cM处,解释的表型变异分别为8.97%、8.12%和7.91%。qγ-A1-1同时在2011年原阳和2015年原阳2个环境下检测到,且均定位在A1(Chr.5)染色体Satt276—Satt364标记区间0.01 cM处,解释的表型变异分别为29.54%和28.23%。qγ-G-1和qγ-A1-1 2个QTL能够稳定遗传。【结论】γ-T最适遗传模型符合MX2-Duplicate-A,即2对重叠作用主基因×加性多基因模型。其遗传同时受到基因型、环境和上位性的影响。检测到γ-T的2个稳定主效QTL,Satt275—Satt038和Satt276—Satt364是共位标记区间。
梁慧珍,许兰杰,董薇,余永亮,杨红旗,谭政委,李磊,刘新梅. 大豆γ-生育酚的混合遗传分析与QTL定位[J]. 中国农业科学, 2020, 53(11): 2149-2160.
LIANG HuiZhen,XU LanJie,DONG Wei,YU YongLiang,YANG HongQi,TAN ZhengWei,LI Lei,LIU XinMei. Mixed Inheritance Analysis and QTL Mapping for γ-Tocopherol Content in Soybean[J]. Scientia Agricultura Sinica, 2020, 53(11): 2149-2160.
表1
RIL群体大豆γ-T含量表型变异"
年份 Year | 平均值Mean (μg·g-1) | 亲本差 P2—P1 (μg·g-1) | RIL变幅RIL range (μg·g-1) | GCV (%) | 遗传率 h2 | ||||
---|---|---|---|---|---|---|---|---|---|
原阳 Yuanyang | 三亚 Sanya | 原阳 Yuanyang | 三亚 Sanya | 原阳 Yuanyang | 三亚 Sanya | 原阳 Yuanyang | 三亚 Sanya | ||
2011 | 111.61 | 100.83 | -51.12 | -48.77 | 25.59—164.15 | 22.85—158.31 | 22.66 | 20.08 | 0.509 |
2012 | 109.92 | 101.18 | -49.90 | -51.23 | 33.65—152.19 | 27.65—161.02 | 20.07 | 16.21 | 0.437 |
2015 | 110.26 | 100.66 | -41.10 | -44.26 | 20.01—158.03 | 31.03—165.06 | 24.61 | 22.12 | 0.498 |
平均Mean | 110.60 | 100.88 | -48.43 | -48.09 | 20.01—164.15 | 22.85—165.06 | 22.59 | 19.65 | 0.488 |
表3
大豆种子γ-T含量相关性分析"
环境 Environment | 原阳2011 Yuanyang2011 | 原阳2012 Yuanyang2012 | 原阳2015 Yuanyang2015 | 三亚2011 Sanya2011 | 三亚2012 Sanya2012 | 三亚2015 Sanya2015 |
---|---|---|---|---|---|---|
原阳2011 Yuanyang2011 | 1 | |||||
原阳2012 Yuanyang2012 | -0.050 | 1 | ||||
原阳2015 Yuanyang2015 | 0.137 | 0.043 | 1 | |||
三亚2011 Sanya2011 | 0.417* | 0.023 | -0.006 | 1 | ||
三亚2012 Sanya2012 | 0.307 | 0.542** | 0.303 | 0.238 | 1 | |
三亚2015 Sanya2015 | 0.307 | -0.243 | 0.047 | 0.356 | 0.361 | 1 |
表4
γ-生育酚最适模型及遗传参数估计结果"
参数 Parameter | γ-生育酚γ-T | 参数 Parameter | γ-生育酚 γ-T | |||||
---|---|---|---|---|---|---|---|---|
原阳Yuanyang | 三亚Sanya | 原阳Yuanyang | 三亚Sanya | |||||
最适模型Optimal model | MX2-Duplicate-A | MX2-Duplicate-A | ||||||
一阶参数1st order parameter | 二阶参数2nd order parameter | |||||||
M | 2011 | 8.6329 | 11.3909 | σp2 | 2011 | 6.3918 | 2.8311 | |
2012 | 10.3476 | 10.4479 | 2012 | 3.8596 | 3.2818 | |||
2015 | 7.0225 | 12.4529 | 2015 | 9.3853 | 3.0353 | |||
iab(i*) | 2011 | 1.6711 | 0.4329 | σmg2 | 2011 | 3.4311 | 0.3695 | |
2012 | 0.9646 | 0.5169 | 2012 | 1.5250 | 0.4158 | |||
2015 | 1.8455 | 0.4010 | 2015 | 5.5337 | 0.3420 | |||
[d] | 2011 | 0.1797 | σpg2 | 2011 | 2.3360 | |||
2012 | 0.2146 | 2012 | 2.7899 | |||||
2015 | 0.1921 | 2015 | 2.6270 | |||||
h2mg (%) | 2011 | 53.68 | 13.05 | |||||
2012 | 39.51 | 12.67 | ||||||
2015 | 58.96 | 11.27 | ||||||
hpg2 (%) | 2011 | 82.51 | ||||||
2012 | 84.99 | |||||||
2015 | 86.55 |
表5
γ-生育酚QTL位置及其参数"
年份 Year | 地点 Location | QTL | 染色体 Chr. | 标记区间 Marker interval | 位置 Position (cM) | 置信区间 Confidence interval | LOD | 加性效应 Additive | R2 (%) |
---|---|---|---|---|---|---|---|---|---|
2011 | 原阳Yuanyang | qγ-A1-1 | A1(5) | Satt276—Satt364 | 12.01 | 7.01—14.35 | 3.71 | 0.622 | 29.54 |
qγ-G-1 | G(18) | Satt275—Satt038 | 0.01 | 0.00—5.15 | 2.50 | -0.590 | 8.97 | ||
三亚Sanya | qγ-A2-1 | A2(8) | Satt315—Satt632 | 0.01 | 0.00—4.78 | 2.54 | -1.235 | 9.45 | |
qγ-A1-2 | A1(5) | Satt364—Satt382 | 22.24 | 18.35—27.11 | 2.63 | 0.621 | 29.55 | ||
2012 | 原阳Yuanyang | qγ-K_2-1 | K_2(9) | Satt264—Sat_044 | 37.68 | 35.40—37.70 | 3.14 | -1.448 | 12.15 |
qγ-A1-3 | A1(5) | Satt382—Satt593 | 55.77 | 48.12—56.69 | 3.18 | -0.212 | 14.20 | ||
三亚Sanya | qγ-A2-2 | A2(8) | Satt315—Satt632 | 2.01 | 0.67—6.17 | 2.73 | -0.711 | 14.31 | |
qγ-G-1 | G(18) | Satt275—Satt038 | 0.01 | 0.00—4.73 | 2.56 | -0.535 | 8.12 | ||
qγ-M-1 | M(7) | Satt220—Satt323 | 75.57 | 73.27—75.60 | 2.73 | -0.658 | 10.04 | ||
2015 | 原阳Yuanyang | qγ-C1-1 | C1(4) | Satt565—Satt578 | 18.82 | 18.80—28.97 | 2.99 | 0.355 | 7.29 |
qγ-A1-1 | A1(5) | Satt276—Satt364 | 12.01 | 8.38—15.03 | 3.81 | 0.613 | 28.23 | ||
三亚Sanya | qγ-G-1 | G(18) | Satt275—Satt038 | 0.01 | 0.00—7.02 | 2.60 | -0.801 | 7.91 |
表6
QTI定位结果比较"
亲本Parent | 性状Traits | 文献References |
---|---|---|
晋豆23×灰布支黑豆Jindou23×Huibuzhi | A1(Satt276, Satt364, Satt382), A2(Satt315), C1(Satt565), G(Satt275), K(Satt264), M(Satt220) | 本研究This study |
Bayfield×合丰25 Bayfield ×Hefeng 25 | A2(Sat_383), C1(Satt565), C2(Satt286), G(Satt199), D1b(Satt266), O(Satt576), J(Satt280) | [19] |
Bayfield×合丰25 Bayfield ×Hefeng 25 | A2(Sat_383), C1(Satt565), C2(Satt286), G(Satt199), D1b(Satt266), O(Satt576), E(Satt355), J(Satt280) | [20] |
Bayfield×Shire | C1(Satt646), E(Satt598), I(Satt354), F(Satt334), H(Satt279), K(Satt552) | [21] |
Bayfield×合丰25 Bayfield ×Hefeng 25 | G(Sat_372), D1b(Satt266), L(Satt561, Satt527), M(Satt567) | [22] |
Essex×ZDD2315 | C2(Satt489), D1a(Sat_346), D1b(AI856415), O(Satt633), K(Satt617, Satt260) | [23] |
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