Ogura雄性不育甘蓝型油菜×白菜、芜菁杂种F1及其回交后代染色体减数分裂行为分析

中国农业科学 ›› 2004, Vol. 37 ›› Issue (09): 1352-1357 .

Ogura雄性不育甘蓝型油菜×白菜、芜菁杂种F1及其回交后代染色体减数分裂行为分析

崔辉梅,曹家树

浙江大学蔬菜研究所

收稿日期:2003-09-16 修回日期:2004-02-16 出版日期:2004-09-20 发布日期:2004-09-20
通讯作者: 曹家树

Analysis of Chromosome Meiotic Behavior of F1 Hybrids and Its Backcross Generations Between Ogura-Type Male-SterileBrassica napus L. and B. campestris L.

浙江大学蔬菜研究所

Received:2003-09-16 Revised:2004-02-16 Online:2004-09-20 Published:2004-09-20

摘要/Abstract

摘要： 以甘蓝型油菜（Brassica napus L.，AACC，2n＝38）Ogura CMS材料与白菜（B. campestris ssp. chinensis Makino, AA，2n＝20）品种新选1号、矮脚黄和芜菁（B. campestris ssp. rapifera Sinsk , AA，2n＝20）品种耐病98-1进行杂交、回交。F1花粉母细胞减数分裂染色体构型为10Ⅱ+9Ⅰ。BC1生长势强的群体中，新选1号和耐病98-1染色体构型多为10Ⅱ+9Ⅰ，矮脚黄的染色体构型以10Ⅱ和10Ⅱ+7Ⅰ占优势；BC1生长势弱的群体中，新选1号和耐病98-1染色体构型多为10Ⅱ+7Ⅰ，矮脚黄的染色体构型多为10Ⅱ+1Ⅰ。BC2生长势强的群体中，新选1号的染色体构型多为10Ⅱ+1Ⅰ，矮脚黄多为10Ⅱ；BC2生长势弱的群体中，新选1号和矮脚黄多为10Ⅱ，耐病98-1多为10Ⅱ+6Ⅰ、10Ⅱ+5Ⅰ。BC3根尖染色体观察新选1号、矮脚黄染色体数达到100%，耐病98-1中染色体数为20的细胞达到78.12%。同时对BC1代不同染色体数在减数分裂Ⅰ的分离规律进行了研究。结果表明，AACC染色体组物种与AA染色体组物种杂交经少数几代回交即可获得后代性状相对稳定的不育系。

关键词: Ogura雄性不育, 白菜, Brassica campestris, 芜菁, B. rapa, 染色体, 减数分裂

Abstract: F1 between female parent of Ogura male sterility Brassica napus L. (AACC，2n＝38) and male parents of B. campestris ssp. chinensis, B. campestris ssp. rapifera (AA, 2n＝20) was made, and BC1 and BC2 generations were produced. The number of somatic and gametic chromosomes of F1 hybrid were 29 and 19, respectively. In the strong growth plant of BC1, the chromosome configuration of Xinxuan1 and Naibing98-1was 10Ⅱ+9Ⅰ,the chromosome configuration of Aijiaohuang were mostly 10Ⅱ and 10Ⅱ+7Ⅰ. In the weak growth plant of BC1, the chromosome configuration of Xinxuan1 and Naibing98-1was 10Ⅱ+7Ⅰ, the chromosome configuration of Aijiaohuang was mostly 10Ⅱ+1Ⅰ. In the strong growth plant of BC2, the chromosome configurations of Xinxuan1 and Aijiaohuang were 10Ⅱ+1Ⅰand 10Ⅱ, respectively, the chromosome configuration of Aijiaohuang was mostly 10Ⅱ. In the weak growth plant of BC2, the chromosome configuration of Xinxuan1 and Aijiaohuang was 10Ⅱ, the chromosome configurations of Naibing98-1 were mostly 10Ⅱ+6Ⅰand 10Ⅱ+5. The chromosome number in Aijiaohuang and Xinxuan1 was 20. The percentage of cell with 2n=20 was 78.12%. Irregular segregation of chromosomes was observed of the BC1 plants during meiosis. These results suggested that the homologous of chromosomes between AACC and AA genome, and it is easy to obtain the CMS in B. compestris by several generations of backcross.

Key words: Ogura male sterility, Chinese cabbage, Brassica campestris, Turnip, Brassica rapa, Chromosome, Meiosis

崔辉梅,曹家树. Ogura雄性不育甘蓝型油菜×白菜、芜菁杂种F1及其回交后代染色体减数分裂行为分析[J]. 中国农业科学, 2004, 37(09): 1352-1357 .

,. Analysis of Chromosome Meiotic Behavior of F1 Hybrids and Its Backcross Generations Between Ogura-Type Male-SterileBrassica napus L. and B. campestris L.[J]. Scientia Agricultura Sinica, 2004, 37(09): 1352-1357 .

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