茶-山苍子间作对茶园土壤微生物群落结构的影响

doi:10.3864/j.issn.0578-1752.2021.18.014

摘要/Abstract

摘要：

【目的】解析山苍子间作条件下茶园土壤微生物群落结构的变化规律及其影响因素,为茶-山苍子间作的土壤生态效应评估提供数据支撑。【方法】综合运用GC-MS、ICP-MS,以及16S、ITS高通量测序技术分别对土壤浸提液成分、土壤矿质营养元素含量、土壤细菌和真菌种群结构进行测定分析。【结果】间作处理显著改变了土壤微生物种群结构,土壤中参与氮（N）、磷（P）和锰（Mn）等过程的功能性细菌丰度显著增加,植物致病细菌和真菌丰度显著降低,根系分布层具有溶解磷（P）功能的伯克氏菌属伯克氏菌丰度是对照的86倍,镰刀菌属镰刀霉丰度比对照降低了73.13%。间作影响了P、铁（Fe）、Mn等土壤矿质营养元素的含量,尤其是P,根系分布层土壤P含量比对照增加76.42%。间作区域土壤分别含有12.80%的樟脑、6.72%的Alpha-松油醇和12.65%的香茅醇等杀菌或抑菌物质。冗余分析表明,间作区域土壤中的樟脑、桉叶油醇、Alpha-松油醇、乙酸冰片酯和香茅醇等次生代谢物与P元素是影响土壤微生物群落结构的主要环境因子。【结论】茶-山苍子间作显著改变了土壤微生物群落结构和丰度,樟脑、Alpha-松油醇和香茅醇等次生代谢产物和P元素是导致土壤微生物群落变化的主要环境因子,茶园及其他农田系统间作植物的选择应重视次生代谢产物的输入,尤其是其中的杀菌或抑菌性成分。

关键词: 山苍子, 茶, 间作, 土壤微生物, 土壤性质

Abstract:

【Objective】 The aim of this study was to analyze the changes of soil microbial community structure and its driving factors, so as to provide data support for soil ecological effect evaluation of Tea-Litsea cubeba intercropping. 【Method】GS-MS, ICP-MS, 16S and ITS sequence analysis were used to determine the soil extract composition, soil mineral element content, soil bacteria and soil fungi community structure.【Result】 The soil microbial community structure was significantly affected by the model of Tea-Litsea cubeba intercropping. The abundance of soil functional bacteria related to N, P and Mn transformation increased significantly, while the abundance of plant pathogenic bacteria and fungi was significantly decreased. In the root distribution layer, the abundance of Burkholderia paraburkholderia was 86 times compared to control, and the abundance of Fusarium graminearum was 73.13% lower than that of the control. Tea-Litsea cubeba intercropping increased the P content of tea plantation soil. In the root distribution layer, P content increased by 76.42%. The soil of Tea-Litsea cubeba intercropping contained some antibacterial substance, including 12.80% camphor, 6.72% Alpha-terpineol, and 12.65% citronellol. Redundancy analysis (RDA) showed that the soil extraction and P in the intercropping area were the main environmental factors affecting the community structure of soil microbes.【Conclusion】 Tea-Litsea cubeba intercropping changed the abundance and community structure of soil microbes, and the camphor, Alpha-terpineol, citronellol and P were the main ecological factors that affected the soil microbial community. The selection of intercropping plants in tea plantations and other farmland systems should pay attention to the secondary metabolites that enter the soil, especially those with killing or antibacterial functional components.

Key words: Litsea cubeba, tea, intercropping, soil microbes, soil properties

郝海平,白红彤,夏菲,郝渊鹏,李慧,崔洪霞,谢晓明,石雷. 茶-山苍子间作对茶园土壤微生物群落结构的影响[J]. 中国农业科学, 2021, 54(18): 3959-3969.

HAO HaiPing,BAI HongTong,XIA Fei,HAO YuanPeng,LI Hui,CUI HongXia,XIE XiaoMing,SHI Lei. Effects of Tea-Litsea Cubeba Intrercropping on Soil Microbial Community Structure in Tea Plantation[J]. Scientia Agricultura Sinica, 2021, 54(18): 3959-3969.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2021.18.014

https://www.chinaagrisci.com/CN/Y2021/V54/I18/3959

图/表 10

表1

图1

图2

图3

图4

表2

表3

图5

图6

表4

参考文献 33

[1]	邓欣, 谭济才, 尹丽蓉, 任佑华, 刘红艳. 不同茶园土壤微生物数量状况调查初报. 茶叶通讯, 2005, 32(2): 7-9.
	DENG X, TAN J C, YIN L R, REN Y H, LIU H Y. Investigation on the quantitative condition of soil microbes in different tea garden. Tea Communication, 2005, 32(2): 7-9. (in Chinese)
[2]	BHATTACHARYYA P N, SARMAH S R, SATYA R S. The role of microbes in tea cultivation. In: Sharma VS, Kumudini MT. Global tea science//Global Tea Science. Burleigh Dodds Science Publishing. India, 2018: 135-167.
[3]	周才碧, 陈文品. 茶园土壤微生物的研究进展. 中国茶叶, 2014, 36(3): 14-15.
	ZHOU C B, CHEN W P. Research progress on soil microorganisms in tea gardens. China Tea, 2014, 36(3): 14-15. (in Chinese)
[4]	张正群, 田月月, 高树文, 许永玉, 黄晓琴, 张丽霞. 茶园间作芳香植物罗勒和紫苏对茶园生态系统影响的研究.
	Ecological effects of intercropping tea with aromatic plant basil and perill in young tea plantation. Journal of Tea Science, 2016, 36(4): 389-395. (in Chinese)
[5]	CULLINGTON J E. WALKER A. Rapid biodegradation of diuron and other phenylurea herbicides by a soil bacterium. Soil Biology Biochemistry, 1999, 31(5): 677-686. doi: 10.1016/S0038-0717(98)00156-4
[6]	李秀英, 赵秉强, 李絮花, 李燕婷, 孙瑞莲, 朱鲁生, 徐晶, 王丽霞, 李小平, 张夫道. 不同施肥制度对土壤微生物的影响及其与土壤肥力的关系. 中国农业科学, 2005, 38(8): 1591-1599.
	LI X Y, ZHAO B Q, LI X H, LI Y T, SUN R L, ZHU L S, XU J, WANG L X, LI X P, ZHANG F D. Effects of different fertilization systems on soil microbe and its relation to soil fertility. Scientia Agricultura Sinica, 2005, 38(8): 1591-1599. (in Chinese)
[7]	钱玮, 陈宏伟, 张铭连, 金琎, 邱劲, 胡翠英. 不同施肥处理对茶树根际微生物群落结构的影响. 基因组学与应用生物学, 2019(3): 1205-1210.
	QIAN W, CHEN H W, ZHANG M L, JIN J, QIU J, HU C Y. Effects of different fertilization treatments on community structure of rhizosphere microbe of camellia sinensis. Genomics and Applied Biology, 2019(3): 1205-1210. (in Chinese)
[8]	刁晓平, 孙英健, 孙振钧, 沈建忠. 安普霉素对不同土壤中微生物活动的影响. 生态环境, 2004, 13(4): 565-568.
	DIAO X P, SUN Y J, SUN Z J, SHEN J Z. Effects of apramycin on microbial activity in different type of soil. Ecology and Environment, 2004, 13(4): 565-568. (in Chinese)
[9]	吴红英, 孔云, 姚允聪, 毕宁宁, 亓丽萍, 付占国. 间作芳香植物对沙地梨园土壤微生物数量与土壤养分的影响. 中国农业科学, 2010, 43(1): 140-150.
	WU H Y, KONG Y, YAO Y C, BI N N, QI L P, FU Z G. Effects of intercropping aromatic plants on soil microbial quantity and soil nutrients in pear orchard. Scientia Agricultura Sinica, 2010, 43(1): 140-150. (in Chinese)
[10]	朱庆松, 刘松虎, 赵海英. 不同覆盖措施对信阳毛尖茶园土壤微生物量动态变化的影响. 河南农业科学, 2010, 39(7): 53-55.
	ZHU Q S, LIU S H, ZHAO H Y. Study of different mulching measures on the dynamic changes of microbial biomass in the tea garden of Xinyang. Journal of Henan Agricultural Sciences, 2010, 39(7): 53-55. (in Chinese)
[11]	杨清平, 毛清黎, 杨新河. 不同生态茶园土壤微生物及脲酶活性研究. 湖北大学学报(自然科学版), 2014, 36(4): 300-302, 306.
	YANG Q P, MAO Q L, YANG X H. The studying of soil microorganism and urease activity of different types of ecological tea garden. Journal of Hubei University (Natural Science Edition), 2014, 36(4): 300-302, 306. (in Chinese)
[12]	徐晨光, 张奇春, 侯昌萍. 外源抗生素对茶园土壤微生物群落结构的影响. 浙江大学学报(农业与生命科学版), 2014, 40(1): 75-84.
	XU C G, ZHANG Q C, HOU C P. Effect of exogenous antibiotics on soil microbial community structure in tea garden. Journal of Zhejiang University (Agriculture & Life Sciences), 2014, 40(1): 75-84. (in Chinese)
[13]	王慧敏. 间作芳香植物对茶园土壤肥力状况的影响[D]. 山东农业大学, 2016.
	WANG H M. Effects of intercropping aromatic plants on the soil fertility in tea plantation[D]. Shandong Agricultural University, 2016. (in Chinese)
[14]	CHEN X X, SONG B Z, YAO Y C, WU H Y, HU J H, ZHAO L L. Aromatic plants play an important role in promoting soil biological activity related to nitrogen cycling in an orchard ecosystem. The Science of the Total Environment, 2014, 472:939-946. doi: 10.1016/j.scitotenv.2013.11.117
[15]	SACCHETTI G, MAIETTI S, MUZZOLI M, SCAGLIANTI M, MANFREDINI S, RADICE M, BRUNI R. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemistry, 2005, 91(4): 621-632. doi: 10.1016/j.foodchem.2004.06.031
[16]	LIANG Q, LI W R, SHI Q S, ZHANG W M. Antifungal activity of Litsea cubeba oil against candida albicans. Biotechnology Bulletin, 2015, 31(10): 205-210.
[17]	LUO M, JIANG L K, ZOU G L. Acute and genetic toxicity of essential oil extracted from Litsea cubeba (Lour.) Pers. Journal of Food Protection, 2005, 68(3): 581-588. doi: 10.4315/0362-028X-68.3.581
[18]	郝海平. 间作山苍子和红豆杉对茶园土壤微生物和微量元素的影响. 博士后出站报告,中国科学院植物研究所, 2019.
	HAO H P. Effects of intercropping Listsea cubeba and Taxus chinensis on soil microbes and trace element in tea plantation. Institute of Botany, The Chinese Academy of Sciences, 2019. (in Chinese)
[19]	HAO H P, LI H, JIANG C D, TANG Y D, SHI L. Ion micro-distribution in varying aged leaves in salt-treated cucumber seedlings. Plant Physiology and Biochemistry, 2018, 129:71-76. doi: 10.1016/j.plaphy.2018.05.022
[20]	汪华. 茶园土壤微生物群落结构对植茶年限、施肥和高温条件的响应研究[D]. 杭州: 浙江大学, 2015.
	WANG H. The response of soil microbial community structure to cultivating age, fertilization and high temperature in tea orchard. Hangzhou: Zhejiang University, 2015. (in Chinese)
[21]	黄伟, 黄欠如, 胡锋, 吴洪生, 李辉信. 红壤溶磷菌的筛选及溶磷能力的比较. 生态与农村环境学报, 2006, 22(3): 37-40.
	HUANG W, HUANG Q R, HU F, WU H S, LI H X. Screening and comparing of phosphorus solubilizing bacteria in red soil. Journal of Ecology and Rural Environment, 2006, 22(3): 37-40.(in Chinese)
[22]	KHAN A A, JILANI G, AKHTAR M S, SAQLAN S M, RASHEED M. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. Journal of Agriculture and Biological Sciences, 2009, 1(1): 48-58.
[23]	BEBBER D P, RICHARDS V R. A meta-analysis of the effect of organic and mineral fertilizers on soil microbial diversity. BioRxiv, 2020. doi: doi.org/10.1101/2020.10.04.325373. doi: doi.org/10.1101/2020.10.04.325373
[24]	ENEBE M C, BABALOLA O O. Effects of inorganic and organic treatments on the microbial community of maize rhizosphere by a shotgun metagenomics approach. Annals of Microbiology, 2020, 70(1): 49. doi: 10.1186/s13213-020-01591-8
[25]	SHIV P, LAL C M, JAIRAM C, SUDHA K, MONIKA K, SANDEEP K, AJAR N Y. Soil Microbiomes for Healthy Nutrient Recycling// YADAV A N, SINGH J, SINGH C, YADAV N. Current Trends in Microbial Biotechnology for Sustainable Agriculture. Environmental and Microbial Biotechnology. Springer, Singapore, 2021.
[26]	SHRIDHAR B S. Nitrogen Fixing Microorganisms. International Journal of Microbiological Research, 2012, 3(1): 46-52.
[27]	赵兴鸽, 张世挺, 牛克昌. 青藏高原高寒草甸土壤真菌多样性与植物群落功能性状和土壤理化特性的关系. 应用与环境生物学报, 2020, 26(1): 1-9.
	ZHAO X G, ZHANG S T, NIU K C. Relationships between soil fungal diversity, plant community functional traits, and soil attributes in Tibetan alpine meadows. Chinese Journal of Applied & Environmental Biology, 2020, 26(1): 1-9. (in Chinese)
[28]	吴晓宗, 王岩. 生物有机肥防治烟草青枯病及对土壤微生物多样性的影响. 中国土壤与肥料, 2019(4): 193-199.
	WU X Z, WANG Y. Research of bio-organic fertilizer on prevention of tobacco bacterial wilt and its effects on soil microbial diversity. Soil and Fertilizer Sciences in China, 2019(4): 193-199. (in Chinese)
[29]	史芳芳, 李向泉. 葡萄根际土壤真菌群落多样性分析. 中国农业科技导报, 2019, 21(7): 47-58.
	SHI F F, LI X Q. Diversity analysis of fungus community in rhizosphere soil of grape. Journal of Agricultural Science and Technology, 2019, 21(7): 47-58. (in Chinese)
[30]	BULGARELLI D, ROTT M, SCHLAEPPE K, THEMAAT E V L, AHMADINEJAD N, ASSENZA F, RAUF P, HUETTEL B, REINHARDT R, SCHMELZER E, PEPLIES J, GLOECHNER F O, AMANN R, EICKHORST T, LEFERT P S. Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota. Nature, 2012, 488(7409): 91-95. doi: 10.1038/nature11336
[31]	李萃邦. 十种植物提取物对茶树叶部病原菌生物活性的研究[D]. 武汉: 华中农业大学, 2017.
	LI C B. Research on biological activities of the extracts from 10 kinds of plants against camellia sinensis pathogens[D]. Wuhan: Huazhong Agricultural University, 2017. (in Chinese)
[32]	SOUZA C M C, PEREIRA JUNIOR S A, MORAES T D S, DAMASCENO J L, AMORIM MENDES S, DIAS H J, STEFANI R, TAVARES D C, MARTINS C H G, CROTTI A E M, MENDES- GIANNINI M J S, PIRES R H. Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells. Medical Mycology, 2016, 54(5): 515-523. doi: 10.1093/mmy/myw003
[33]	PARK S N, LIM Y K, FREIRE M O, CHO E, JIN D C, KOOK J K. Antimicrobial effect of linalool and α-terpineol against periodontopathic and cariogenic bacteria. Anaerobe, 2012, 18(3): 369-372. doi: 10.1016/j.anaerobe.2012.04.001

处理编号 Treatment number	土层 Soil layer
处理编号 Treatment number	表土层 Top soil layer (0—10 cm)	根系分布层 Root distribution layer (10—30 cm)
1	CK.1	CK.2
2	Lit.1	Lit.2

微生物 Microbes		潜在功能 Potential functions	丰度 OTU number
微生物 Microbes		潜在功能 Potential functions	CK.1	Lit.1	CK.2	Lit.2
细菌 Bacteria	黄单胞杆菌Norank xanthomonadales	植物病原菌 Plant pathogens	218	0	493	0
	伯克氏菌 Burkholderia paraburkholderia	溶解磷;生物降解 Decomposition and release P; Biodegradation	1	63	0	86
	黄色菌 Norank xanthobacteraceae	五氯硝基苯降解 Pentachloronitrobenzene degradation	2	1523	8	851
	慢生根瘤菌 Bradyrhizobium	固氮 Nitrogen fixation	269	662	315	385
	土微菌 Pedomicrobium	锰氧化 Manganese oxidantion	0	241	1	214
	真杆菌 Norank acidobacteria	还原硝酸盐 Nitrate reduction	1	484	3	604
	栖热菌属 Acidothermus	还原硝酸盐 Nitrate reduction	18	84	121	49
	浮霉状菌属 Planctomyces	缺氧条件下氧化铵生成氮气 Amine generates nitrogen in anoxic environment	0	78	2	63
真菌 Fungi	镰刀霉 Fusarium graminearum	致病菌 Pathogenic bacteria	4729	695	3122	839
	毛壳菌 Chaetomium	纤维素分解 Cellulose breakdown	1	624	4	255
	拟康宁木霉 Trichoderma koningiopsis	生物防治菌 Biocontrol bacteria	41	1589	29	6942
	山野壳菌 Paraphaeosphaeria	病原真菌 Pathogenic fungi	145	14	216	33

峰 Peak	时间 RT	面积 Area	峰面积比例 Peak area ratio (%)	名称 Name
1	4.42	108958	3.01	苯乙烯 Phenylethylene
2	6.01	135087	3.73	桉叶油醇 Cineole
3	7.17	463228	12.80	樟脑 (+)-2-Bornanone
4	7.64	243391	6.72	Alpha-松油醇 Alpha-Terpineol
5	7.99	457853	12.65	香茅醇 Citronellol
6	8.30	90066	2.49	乙酸冰片酯 Bornyl acetate
7	8.74	103561	2.86	乙酸香茅酯 Citronellol acetate
8	9.00	61857	1.71	丙酸 Propanoic acid
9	9.35	293552	8.11	羟基香茅醛 4-Octene-2,7-diol
10	11.08	48177	1.33	2-甲基十七烷 Dibuyl phthalate
11	12.10	132366	3.66	邻苯二甲酸二异丁酯 1,2-Benzenedicarboxylic acid
12	12.68	280323	7.74	邻苯二甲酸二正丁酯 Dibuyl phthalate
13	14.37	167173	4.62	棕榈酰胺 Hexadecanamide
14	16.13	1034060	28.57	油酸酰胺 Oleamide

环境因子 Name	关联度 Explain	Pseudo -F值 Pseudo-F	P值 P
Ci 桉叶油醇 Cineole	48.4	3.7	0.03*
Alp Alpha-松油醇 Alpha-Terpineol	48.1	3.7	0.01*
Ph 苯乙烯 Phenylethylene	48.0	3.7	0.018*
Bo 樟脑 (+)-2-Bornanone	48.0	3.7	0.034*
Cit 香茅醇 Citronellol	47.8	3.7	0.028*
P 磷 Phosphorus	47.8	3.7	0.04*
Fe 铁 Iron	46.7	3.5	0.068
Zn 锌 Zinc	46.1	3.4	0.06
Mn 锰 Manganese	44.4	3.2	0.112
Mg 镁 Magnesium	43.5	3.1	0.06
Cu 铜 Copper	36.1	2.3	0.114