中国农业科学 ›› 2020, Vol. 53 ›› Issue (20): 4248-4258.doi: 10.3864/j.issn.0578-1752.2020.20.013

• 园艺 • 上一篇    下一篇

暗期短暂远红光处理对南瓜幼苗生长、细胞形态和激素含量的影响

刘齐1(),梅延豪1,李琦1,马宏秀3,武永军2,杨振超1()   

  1. 1西北农林科技大学园艺学院/农业农村部西北设施园艺工程重点实验室,陕西杨凌 712100
    2西北农林科技大学生命学院,陕西杨凌712100
    3杨凌凌宇现代农业研究院,陕西杨凌 712100
  • 收稿日期:2020-03-14 接受日期:2020-05-12 出版日期:2020-10-16 发布日期:2020-10-26
  • 通讯作者: 杨振超
  • 作者简介:刘齐,E-mail: lq821798304@163.com
  • 基金资助:
    国家重点研发计划(2018YFD0201205-2);陕西省农业厅农业科技创新集成推广项目(NYKJ-2018-YL22)

Effects of End of Day Far-Red Light on Growth, Histiocyte Morphology and Phytohormones Content of Pumpkin Seedlings

LIU Qi1(),MEI YanHao1,LI Qi1,MA HongXiu3,WU YongJun2,YANG ZhenChao1()   

  1. 1College of Horticulture, Northwest A&F University/Key Laboratory of Protected Horticultural Engineering in Northwestern China, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi
    2College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi
    3Lingyu Modern Agricultural Research Institute, Yangling 712100, Shaanxi
  • Received:2020-03-14 Accepted:2020-05-12 Online:2020-10-16 Published:2020-10-26
  • Contact: ZhenChao YANG

摘要:

【目的】研究暗期前短暂远红光处理对南瓜幼苗生长形态、组织细胞形态和相关激素水平的影响,为远红光在农业上的应用提供理论依据。【方法】以南瓜品种‘日本雪松’为试验材料,分别在暗期前给予2(T1)、4(T2)、6(T3)、8(T4)、10(T5)和12(T6)mmol·m-2·d-1的远红光处理,以无远红光处理为对照(CK),测定植株生长形态下胚轴细胞形态以及生长素(IAA)、玉米素(ZT)、赤霉素(GA3)与油菜素内酯(BR)含量。【结果】在暗期短时外施远红光能显著提高南瓜幼苗下胚轴长度和株高,对植株茎粗,地上、地下部干/鲜重无显著影响;2、4、6、8、10和12 mmol·m-2·d-1远红光处理的下胚轴薄壁细胞轴向长度分别比CK显著增加34.6%、20.7%、31.3%、25.6%、32.8%和20.9%;下胚轴厚角组织厚度分别比CK显著增加19.6%、22.4%、21.2%、23.9%、19.6%和28%;经暗期前远红光处理后,南瓜幼苗根中生长素(IAA)含量,下胚轴中生长素(IAA)、赤霉素(GA3)、玉米素(ZT)含量,子叶中生长素(IAA)、赤霉素(GA3)、油菜素内酯(BR)含量以及真叶中生长素(IAA)与油菜素内酯(BR)含量均得到显著提高。【结论】暗期前短时远红光处理可能通过提高激素含量,进而改变细胞形态,促进下胚轴伸长生长。

关键词: 远红光, 南瓜幼苗, 下胚轴, 细胞形态, 激素, 远红光调控

Abstract:

【Objective】This study was aimed to understand the effects of growth, histiocyte morphologic and phytohormone levels of pumpkin seedlings under different doses of End-Of-Day Far-Red light (EOD-FR) treatment, so as to provide a theoretical basis for the application of far-red in agricultural industry. 【Method】 Taken the pumpkin (cv. Japanese cedar) as experimental material, the growth state of hypocotyl’s histiocyte morphology and the contents of auxin (IAA), zeatin (ZT), gibberellin (GA3) and brassinolide (BR) were measured under 0 (CK), 2 (T1) , 4 (T2) , 6 (T3) , 8 (T4) , 10 (T5) and 12 (T6) mmol·m-2·d-1dose far-red light treatments, respectively. 【Result】 The hypocotyl length and plant height of pumpkin seedlings were significantly increased by EOD-FR treatment, while there was no significant effect on the diameter, dry and fresh weight of pumpkin stem. Compared with the control group, the lengths of hypocotyl’s parenchyma cell of each treatment were significantly increased by 34.6%, 20.7%, 31.3%, 25.6%, 32.8% and 20.9%, respectively; the collenchyma's thickness were significant increased by 19.6%, 22.4%, 21.2%, 23.9%, 19.6% and 28%, respectively. After EOD-FR treatment, the contents of IAA in roots of pumpkin seedlings significantly increased, and the contents of IAA, GA3 and ZT in hypocotyls, IAA, GA3 and BR in cotyledons, IAA and BR in euphylla were also increased. 【Conclusion】Through increasing the hormone levels under EOD-FR treatment, the histiocyte morphology could be changed, and the hypocotyl elongation could be increased.

Key words: far-red light, pumpkin seedlings, hypocotyl, cell morphology, phytohormone, regulative of far-red light