中国农业科学 ›› 2018, Vol. 51 ›› Issue (5): 952-963.doi: 10.3864/j.issn.0578-1752.2018.05.013

• 园艺 • 上一篇    下一篇

根施褪黑素对NaCl胁迫下葡萄内源褪黑素及叶绿素荧光特性的影响

卞凤娥,肖秋红,郝桂梅,孙永江,陆文利,杜远鹏,翟衡   

  1. 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安 271018
  • 收稿日期:2017-07-20 出版日期:2018-03-01 发布日期:2018-03-01
  • 通讯作者: 翟衡,Tel:0538-8241335;E-mail:zhaih@sdau.edu.cn
  • 作者简介:卞凤娥,E-mail:bianfengeee@163.com
  • 基金资助:
    国家现代葡萄产业技术体系建设专项(CARS-29)、长江学者和创新团队发展计划(IRT15R42)

Effect of Root-Applied Melatonin on Endogenous Melatonin and Chlorophyll Fluorescence Characteristics in Grapevine Under NaCl Stress

BIAN FengE, XIAO QiuHong, HAO GuiMei, SUN YongJiang, LU WenLi, DU YuanPeng, ZHAI Heng   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong
  • Received:2017-07-20 Online:2018-03-01 Published:2018-03-01

摘要: 【目的】研究NaCl胁迫下内源褪黑素(melatonin,MT)及其关键代谢物的响应以及施加外源MT对NaCl胁迫下葡萄叶片的光抑制缓解作用,为MT的应用提供参考。【方法】以一年生盆栽‘威代尔’葡萄(Vitis vinifera cv. Vidal Blanc)为试材,进行100 nmol·L-1 NaCl和100 nmol·L-1 NaCl+MT处理,测定不同处理植株、不同器官内源MT、MT合成前体物质5-羟色胺(5-hydroxytryptamine,5-HT)和MT的主要代谢产物2-羟基褪黑素(2-Hydroxymelatonin,2-OHMel)的含量,分析根施MT对NaCl胁迫下叶片叶绿素荧光特性及内源MT代谢的影响。【结果】受NaCl胁迫后,植株不同器官的内源MT、5-HT及2-OHMel与清水对照相比有明显的时空变化,胁迫7 d时以根系的MT含量最高,其次是嫩梢,成叶中的最低;随着胁迫时间的延长,根系中的MT含量大幅度下降,而嫩梢中的5-HT含量增加。根施外源MT后与单纯的NaCl胁迫相比,显著提高了前期成叶中的5-HT含量以及后期新根中的MT含量,嫩梢和成叶中2-OHMel的含量。NaCl胁迫后快速叶绿素荧光曲线(OJIP)形状发生明显改变,最大荧光(Fm)及叶片最大光化学效率(Fv/Fm)显著下降了31.6%和11.6%,非光化学淬灭(NPQ)显著升高,光系统I(PSI)和光系统II(PSII)的线性电子传递速率ETR(I)和ETR(II)均显著下降;而根施MT后,叶片Fm和Fv/Fm的分别升高了12.9%和7.3%,OJIP曲线中K点和J点分别下降了23.6%和11.3%,增加了叶片对光能的利用效率,线性电子传递速率明显提高。【结论】NaCl胁迫严重抑制葡萄叶片光系统活性,但促进了植株中褪黑素的合成;根施MT缓解了光系统活性的抑制程度,促进MT在植株各器官中的代谢和分配,缓解NaCl胁迫对葡萄叶片光合作用的伤害程度。

关键词: NaCl胁迫, 葡萄, 叶绿素荧光, 褪黑素, 5-羟色胺, 2-羟基褪黑素

Abstract: 【Objective】 The content of the endogenous MT, the key metabolites, and the effect of photoinhibition on the leaf were studied in grapevine under NaCl stress, and the results will provide a reference for the application of MT in the future.【Method】One-year old potted ‘Vidal Blanc’ grapevines (Vitis vinifera cv. Vidal Blanc) were used as materials. The grapevines were irrigated with NaCl (100 nmol·L-1 ) and NaCl+MT (100 nmol·L-1), and then the content and distribution of the endogenous MT, MT precursors serotonin (5-hydroxytryptamine, 5-HT), and then the main metabolites of melatonin 2-hydroxymelatonin (2-OHMel) in different organs of different treatment plants, and the chlorophyll fluorescence characteristics in leaves and the metabolism of endogenous MT were measured and analyzed.【Result】The results showed that there were obvious temporal and spatial changes in the content of endogenous MT, 5-HT and 2-OHMel in different organs of plants under NaCl stress, and the MT content of roots reached the peak value after 7 days treatment, followed by the shoots, and the content of leaves were the lowest. With the prolongation of the stress time, the MT content in the roots decreased significantly, while the 5-HT content in the shoots increased. Compared with the only NaCl stress, the 5-HT content in the leaves, the MT content in the roots and the contents of 2-OHMel in the shoots and leaves increased significantly after root-applied MT. The shape of OJIP curve was significantly changed, Fm and leaf maximum photochemical efficiency (Fv/Fm) significantly decreased by 31.6% and 11.6%, the non-photochemical quenching (NPQ) was significantly increased, and the linear electron transport rate of PSI (ETR(I)) and PSII (ETR(II)) were significantly decreased under NaCl stress. While the Fm and Fv/Fm in leaves were reduced by 12.9% and 7.3%, the increase of K and J points in the OJIP curve was significantly reduced by 23.6% and 11.3%, and the utilization efficiency of the light linear and electron transfer rate is significantly improved under NaCl stress and MT treatment.【Conclusion】NaCl stress induced the inhibition of photosystem activity in grapevine leaves, but promoted the synthesis of MT in the plant. Photoinhibition was alleviated under MT treatment, root-applied MT promoted the metabolism and distribution of MT in various organs of plants, alleviated the damaged degree of photosynthesis of grapevine leaves by NaCl stress.

Key words: NaCl stress, grapevine, chlorophyll fluorescence, melatonin, 5-hydroxytryptamine, 2-hydroxymelatonin