伊拉兔宰后肌糖原变化及其与兔肉品质的相关性

doi:10.3864/j.issn.0578-1752.2014.04.022

摘要/Abstract

摘要： 【目的】探讨伊拉兔宰后36 h内肌糖原变化规律及与肉品品质变化的相关性。【方法】伊拉兔经屠宰之后，肌肉内的糖原会在一定时间内酵解转化为乳酸，从而改变胴体的pH值，并进一步影响肉品的持水力、剪切力、肉色等品质指标。以伊拉兔背最长肌（LD）和左后腿肌（LL）为原料，用试剂盒法分别测定了伊拉兔宰后45 min、4 h、8 h、12 h、24 h、36 h肌糖原含量的变化，并对兔宰后相应时间点的肉品品质指标如pH值、蒸煮损失、滴水损失、剪切力、色泽变化进行了检测与分析。此外，将主要设计因子（pH、蒸煮损失、滴水损失、总损失、剪切力、L*、a*、b*、C*）作为X变量，以肌糖原的含量作为Y变量，用偏最小二乘回归分析法（PLS2）对伊拉兔宰后LD和LL肉品品质与肌糖原变化规律的相关性进行探讨。【结果】伊拉兔宰后36 h内部位LD和LL的肌糖原含量均下降显著（P＜0.05），二者分别从屠宰初期的（2.61±0.50）mg•g-1和（2.77±0.55）mg•g-1减少为宰后36 h的（0.94±0.05）mg•g-1和（0.99±0.07）mg•g-1。与此同时，伊拉兔LD和LL的pH分别从宰后初期的（6.91±0.02）和（6.85±0.04）下降至（5.62±0.09）和（5.61±0.09）。在此期间，随着pH的下降，伊拉兔肉品品质指标也发生了相应地改变，如剪切力、亮度L*值、红度a*值降低；蒸煮损失、滴水损失、总损失、b*值和C*值升高。PLS2分析显示，以上各肉品品质指标与兔宰后肌糖原含量变化的相关性均较大，部位LD和LL的回归系数R值分别达0.796—0.972和0.890—0.996。其中，2个部位的总损失指标（蒸煮损失与滴水损失之和）较其他肉品品质的差异来源更为显著。此外，可以用以上兔肉宰后品质指标为自变量，运用Unscrambler建立回归方程对肌糖原变化评分较好地进行预测。【结论】伊拉兔宰后36 h内肌糖原下降显著，与其间肉品品质的变化密切相关，可利用其酵解规律与各品质指标的相关性建立回归方程，从而对肌糖原含量变化进行较好地预测。

关键词: 伊拉兔 , 肌糖原 , 兔肉品质 , 相关性

Abstract: 【Objective】This paper is to discuss the relevance between the change of muscle glycogen and meat quality of IRA rabbit within 36 h slaughter. 【Method】After slaughter, the intramuscular glycogen of IRA rabbit occurs glycolysis reaction into lactic acid in a certain period of time, thus changing the pH value of the carcass, and further affecting meat quality indicators, such as water holding capacity, shear force, color and so on. The longissimus dorsi (LD) muscle and the left leg (LL) muscle were used as raw materials and the variation in content of muscle glycogen of IRA rabbit at 45 min, 4 h, 8 h, 12 h, 24 h and 36 h slaughter period was measured, respectively, by kit method, and the related meat quality indexes during corresponding post-mortem, such as pH, cooking loss, drip loss, shear force and color changes were all determined. In addition, the correlation between meat quality and muscle glycogen variation of IRA rabbit from LD and LL during the 36 h post-mortem were discussed, by the way of partial least squares regression analysis (PLS2), making the main design factors (pH, cooking loss, drip loss, total loss, shear force, L*, a*, b*, C*) as X variables, and muscle glycogen content as Y variables. 【Result】The muscle glycogen content of LD and LL of IRA rabbit decreased significantly (P＜0.05) within 36 h slaughter, reduced from the initial (2.61±0.50) mg•g-1 and (2.77±0.55) mg•g-1 to (0.94±0.05) mg•g-1 and (0.99 ± 0.07) mg•g-1 after 36 h slaughter, respectively. At the same time, the pH value of LD and LL of Iraq rabbit meat decreased from (6.91 ± 0.02) and (6.85 ± 0.04) in the initial post-mortem to (5.62 ± 0.09) and (5.61 ± 0.09) after 36 h slaughter, respectively. In the meantime, the meat quality index of Ira rabbit occurred corresponding changes as the pH value decreased, such as shear strength, lightness value L*, redness value a* decreased; cooking loss, drip loss, total losses, the value of b* and the value of C* increased. PLS2 analysis shows that the correlations between the above meat quality indicators and muscle glycogen content of IRA rabbit during 36 h post-mortem were significant, and the R regression value of location LD and LL reached up to 0.796-0.972 and 0.890-0.996, respectively. Among them, the total loss indicators (sum of cooking loss and drip loss) of the two parts (LD and LL) were provided with more significant sources of variation, compared to other meat quality indexes. What’s more, these meat quality indicators after slaughter can be used as independent variables to predict the score of muscle glycogen changes of IRA rabbit successfully by using Unscrambler regression equation. 【Conclusion】The decrease of muscle glycogen of IRA rabbit within 36 h slaughter period was significant, and the variation was closely related to the corresponding meat quality. Therefore, the correlation between glycolysis law and meat quality indicator can be used to set regression equation for the better prediction of muscle glycogen content changes.

Key words: IRA rabbit , muscle glycogen , rabbit meat quality , correlation

薛山, 贺稚非, 李洪军. 伊拉兔宰后肌糖原变化及其与兔肉品质的相关性[J]. 中国农业科学, 2014, 47(4): 814-822.

XUE Shan, HE Zhi-Fei, LI Hong-Jun. Variation of Muscle Glycogen of IRA Rabbit After Slaughter and Its Correlation with Changes of Rabbit Meat Quality[J]. Scientia Agricultura Sinica, 2014, 47(4): 814-822.

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参考文献

[1]Zotte A D, Szendr? Z. The role of rabbit meat as functional food. Meat Science, 2011, 88(3): 319-331.

[2]Hernández P. Enhancement of Nutritional Quality and Safety in Rabbit Meat. 9th World Rabbit Congress, Verona, Italy, 2008: 367-383.

[3]薛山, 贺稚非, 李洪军. 兔肉胶原蛋白特性及评定方法研究. 食品工业科技, 2013, 34(4): 372-377.

Xue S, He Z F, Li H J. Characteristics and evaluation method of collagen from rabbit meat. Science and Technology of Food Industry, 2013, 34(4): 372-377. (in Chinese)

[4]Scheffler T L, Gerrard D E. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Science, 2007, 77(1): 7-16.

[5]Choe J H, Choi Y M, Lee S H, Shin H G, Ryu Y C, Hong K C, Kim B C. The relation between glycogen, lactate content and muscle fiber type composition, and their influence on postmortem glycolytic rate and pork quality. Meat Science, 2008, 80(2): 355-362.

[6]Essen-Gustavsson B, Karlsson A, Lundstrom K, Enfalt A C. Intramuscular fat and muscle fibre lipid contents in halothane- gene-free pigs fed high or low protein diets and its relation to meat quality. Meat Science, 1994, 38(2): 269-277.

[7]Ryu Y C, Kim B C. Comparison of histochemical characteristics in various pork groups categorized by postmortem metabolic rate and pork quality. Journal of Animal Science, 2006, 84(4): 894-901.

[8]谢晓红, 郭志强, 秦应和. 我国肉兔产业现状及发展趋势. 中国畜牧杂志, 2011(4): 34-38.

Xie X H, Guo Z Q, Qin Y H. The feeding rabbit industry situation and development trend in our country. Chinese Journal of Animal Husbandry, 2011(4): 34-38. (in Chinese)

[9]FAOSTAT (2010). http://faostat.fao.org/site/291/default.aspx.

[10]王文智, 武拉平, 秦应和. 我国兔肉产量、需求波动及与价格的关系分析. 中国养兔, 2011(1): 39-42.

Wang W Z, Wu L P, Qin Y H. Analysis of the rabbit meat production, demand fluctuations and the relationship with price. Chinese Journal of Rabbit Farming, 2011(1): 39-42. (in Chinese)

[11]秦应和. 国家兔产业技术体系“十二五”建设规划//首届(2011)中国兔业发展大会会刊. 山东: 首届(2011)中国兔业发展大会组委会, 2011: 34-37.

Qin Y H. The "twelfth five-year" construction plan of national rabbit industry technology system// The Proceedings of Conference of the First (2011) Rabbit Industry Development in China. Shandong: the organizing committee of the first (2011) Chinese rabbit industry development conference, 2011: 34-37. (in Chinese)

[12]段艳宇, 周利华, 袁飞, 李琳，麻骏武. 猪肌肉糖原酵解潜能及其组分与肉质、脂肪沉积和血液性状的相关性. 江西农业大学学报, 2010, 32(6): 1224-1229.

Duan Y Y, Zhou L H, Yuan F, Li L, Ma J W. Correlation of muscle glycolytic potential and its components with meat quality, fat deposition and blood indexes in pigs. Aeta Agriculturae Universitatis Jiangxiensis,2010, 32(6): 1224-1229. (in Chinese)

[13]Huff-Lonergan E, Baas T J, Malek M, Dekkers J C, Prusa K, Rothschild M F. Correlations among selected pork quality traits. Journal of Animal Science, 2002, 80(3): 617-627.

[14]Hamilton D N, Miller K D, Ellis M, McKeith F K, Wilson E R. Relationships between longissimus glycolytic potential and swine growth performance, carcass traits, and porkquality. Journal of Animal Science, 2003, 81(9): 2206-2212.

[15]Moeller S J, BaasT J, LeedsT D, Emnett R S, Irvin K M. Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire-sired pigs. Journal of Animal Science, 2003, 81(2): 402-410.

[16]Hamilton D N, Ellis M, Hemann M D, Mckeith F K, Miller K D, Purser K W. The impact of Longissmus glycolytic potential and short-term feeding of magnesium sulfate heptahydrate prior to slaughter on carcass characteristics and pork quality. Journal of Animal Science, 2002, 80(6): 1586-1592.

[17]Savell J W, Mueller S L, Baird B E. The chilling of carcasses. Meat Science, 2005, 70(3): 449-459.

[18]Zhu X, Ruusunen M, Gusella M, Zhou G, Puolanne E. High post-mortem temperature combined with rapid glycolysis induces phosphorylase denaturation and produces pale and exudative characteristics in broiler Pectoralis majormuscles. Meat Science, 2011, 89(2): 181-188.

[19]Ramírez J A, Oliver M À, Pla M, Guerrero L, Ariňo B, Blasco A, Pascual M, Gil M. Effect of selection for growth rate on biochemical, quality and texture characteristics of meat from rabbits. Meat Science, 2004, 67(4): 617-624.

[20]Yu L H, Lee E S, Jeong J Y, Paik H D, Choi J H, Kim C J. Effects of thawing temperature on the physicochemical properties of pre-rigor frozen chicken breast and leg muscles. Meat Science, 2005, 71(2): 375-382.

[21]Yu L H, Lim D G, Jeong S G, In T S, Kim J H, Ahn C N, Kim C J, Park B Y. Effects of temperature conditioning on postmortem changes in physico-chemical properties in Korean native cattle (Hanwoo). Meat Science, 2008, 79(1): 64-70.

[22]Boccard R, Butcher L, Casteels E, Cosentino E, Dransfìeld E, Hood D E, Joseph R L, MacDougall D B, Rhodes D N, Schön I, Tinbergen B J, Touraille C. Procedures for measuring meat quality characteristics in beef production experiments. Report of a working group in the Commission of the European Communities’ (CEC), beef production research programme. Livestock Production Science, 1981, 8(5): 385-397.

[23]Bowker B C, Grant A L, Forrest J C, Gerrard D E. Muscle metabolism and PSE pork. Journal of Animal Science, 2000, 79(1): 1-8.

[24]Pösö A R, Puolanne E. Carbohydrate metabolism in meat animals. Meat Science, 2005, 70(3): 423-434.

[25]Ryu Y C, Kim B C. The relationship between muscle fiber characteristics, postmortem metabolic rate, and meat quality of pig longissimus dorsi muscle. Meat Science, 2005, 71(2): 351-357.

[26]Metzger S Z, Bianchi M, Cavani C, Petracci M, Szab? A, Gyovai M, Bir?-Németh E, Radnai I, Szendr?. Effect of nutritional status of rabbit kits on their productive performance, carcass and meat quality traits. Livestock Science, 2011, 137(1/3): 210-218.

[27]徐舶. 花•马杂交F1鹿肉的品质特性研究[D]. 南京: 南京农业大学, 2006.

Xu B. Study on the Characteristcs in Venison of Hybrid F1 Deer[D]. Nanjing: Nanjing Agricultural University, 2006. (in Chinese)

[28]刘正远, 袁缨. 影响肉质的生物化学和组织学因素. 饲料工业, 2005, 26(11): 44-46.

Liu Z Y, Yuan Y. Analyse and application of the histology and biochemistry factors determinating meat quality. Feed Industry, 2005, 26(11): 44-46. (in Chinese)

[29]冯京海, 张敏红, 郑姗姗, 谢鹏, 李军乔. 日循环高温对肉鸡线粒体活性氧产生量、钙泵活性及胸肌品质的影响. 畜牧兽医学报, 2006, 37(12): 1304-1311.

Feng J H, Zhang M H, Zheng S S, Xie P, Li J Q. The effect of cyclic high temperature on mitochondrial ROS production, Ca2+-ATPase activity and breast meat quality of broilers. Acta Veterinaria et Zootechnica Sinica, 2006, 37(12): 1304-1311. (in Chinese)

[30]佟辉, 陈杰, 杨晓静, 邵根宝, 贾超, 陈伟华, 赵茹茜. 背最长肌肌红蛋白mRNA 表达与肉色表观性状之间的相关. 食品与生物技术学报, 2008, 27(3): 94-98.

Tong H, Chen J, Yang X J, Shao G B, Jia C, Chen W H, Zhao R Q. Relationship between level of myoglobin mRNA and phenotypic parameters of meat colour in longissimus dorsi. Journal of Food Science and Biotechnolog, 2008, 27(3): 94-98. (in Chinese)

[31]贾小翠, 李春保, 徐幸莲, 周光宏. 禁食时间对宰后早期鸡肉持水力和嫩度的影响. 食品科学, 2011, 32(7): 1-6.

Jia X C, Li C B, Xu X L, Zhou G H. Effect of fasting time on water-holding capacity and tenderness of chicken breast during early postmortem period. Food Science, 2011, 32(7): 1-6. (in Chinese)

[32]李超, 王道营, 卞欢, 高峰, 徐为民, 周光宏, 吕鑫. 肉鸭屠宰加工过程中色泽、剪切力、pH值及保水性的变化. 江苏农业学报, 2011, 27(4): 868-871.

Li C, Wang D Y, Bian H, Gao F, Xu W M, Zhou G H, Lü X. Changes of color, shear force, pH value and water holding capacity of meat duck during slaughtering. Jiangsu Journal of Agricultural Science, 2011, 27(4): 868-871. (in Chinese)

[33]吴菊清, 李春保, 周光宏, 徐幸莲, 鞠秀香, 陈怡晨. 宰后成熟过程中冷却牛肉、猪肉色泽和嫩度的变化. 食品科学, 2008, 29(10): 136-139.

Wu J Q, Li C B, Zhou G H, Xu X L, Ju X X, Chen Y C. Changes of meat color and tenderness of chilled beef and pork during postmortem aging. Food Science, 2008, 29(10): 136-139. (in Chinese)

[34]Lee Y S, Owens C M, Meullenet J F. Changes in tenderness, color, and water holding capacity of broiler breast meat during postdeboning aging. Journal of Food Science, 2009, 74(8): 449-454.

[35]李泽, 马霞, 靳烨. 不同年龄和部位羊肉中AMPK活性与糖酵解的差异. 食品与发酵工业, 2010, 36(1): 184-186.

Li Z, Ma X, Jin Y. Difference of AMPK and glycolysis in different ages and parts of lamb. Food and Fermentation Industries, 2010, 36(1): 184-186. (in Chinese)

[36]李梦云, 陈代文, 张克英. 肌糖原含量与猪生产性能、胴体品质及肉质性状间的关系. 中国畜牧杂志, 2008, 44(1): 25-29.

Li M Y, Chen D W, Zhang K Y. Relationships between muscle glycogen and swine growth performance, carcass taits and pork quality. Chinese Journal of Animal Husbandry, 2008, 44(1): 25-29. (in Chinese)

[37]Wheeler T L, Savell J W, Cross H R, Lunt D K, & Smith S B. . Mechanisms associated with the variation in tenderness of meat from brahman and hereford cattle. Journal of Animal Science, 1990, 68(12): 4206-4220.

[38]Rosenvold K, Petersen J S, Lwerke H N, Jensen S K, Therkildsen M, Karlsson A H, Møller H S, Andersen H J. Muscle glycogenstores andmeat quality asaffectedby strategic finishing feeding of slaughter pigs. Journal of Animal Science, 2001, 79(2): 382-391.

[39]Morton J D, Bickerstaffe R, Le Couteur C E, Keeley G M. Comparison of different types of low voltage electrical stimulation on tenderisation: Interaction with stress and calpains// Proceedings 43rd International Congress of Meat Science and Technology. Auckland, New Zealand, 1997: 672-673.

[40]门小明, 邓波, 徐子伟, 刘敏华, 齐珂珂. 猪宰后肌肉非乳酸供能特点及其与肉质性状相关性. 中国农业科学, 2011, 44(7): 1457-1465.

Men X M, Deng B, Xu Z W, Liu M H, Qi K K. Characteristics of ATP-CP system status in postmortem muscle and their associations with pork quality traits. Scientia Agricultura Sinica, 2011, 44(7): 1457-1465. (in Chinese)

[41]周光宏, 徐幸莲. 肉品学. 北京: 中国农业科技出版社, 1999.

Zhou G H, Xu X L. Meat Science. Beijing: China Agricultural Science and Technology Press, 1999. (in Chinese)

[42]Fernandez X, Lefaucheur L, Candek M. Comparative study of two classifications of muscle fibres: Consequences for the photo-metric determination of glycogen according to fibre type in red and white muscle of pig. Meat Science, 1995, 41(2): 225-235.

[43]Ozawa S, Mitsuhashi T, Mitsumoto M, Matsumoto S, Itoh N, Itagaki K, Kohno Y, Dohqo T. The characteristics of muscle fiber types of longissimus thoracis muscle and their influences on the quantity and quality of meat from Japanese Black steers. Meat Science, 2000, 54(1): 65-70.

[44]Ryu Y C, Choi Y M, Kim B C. Variations in metabolite contents and protein denaturation of thelongissimus dorsi muscle in various porcine quality classifications and metabolic rates. Meat Science, 2005, 71(3): 522-529.

[45]李洪军, 黄业传, 贺稚非, 李凤. 猪肉制品冷藏中感官特性和挥发性物质变化的回归分析. 中国农业科学, 2012, 45(1): 142-152.

Li H J, Huang Y C, He Z F, Li F. Regression analysis of sensory characteristics and volatile compounds in pork product during cold-storage. Scientia Agricultura Sinica, 2012, 45(1): 142-152. (in Chinese)