中国农业科学 ›› 2013, Vol. 46 ›› Issue (21): 4506-4514.doi: 10.3864/j.issn.0578-1752.2013.21.013

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甜高粱SAI基因的表达与茎秆糖分积累的相关性分析

 聂元冬1, 钟海丽1, 顿宝庆1, 叶凯2, 王智1, 朱莉3, 李桂英1   

  1. 1.中国农业科学院作物科学研究所/生物质能源研究中心/农作物基因资源与基因改良国家重大科学工程,北京100081
    2.新疆农业科学院生物质能源研究所,乌鲁木齐830091
    3.中国农业科学院生物技术研究所,北京 100081
  • 收稿日期:2013-05-15 出版日期:2013-11-01 发布日期:2013-05-15
  • 通讯作者: 通信作者李桂英,Tel:010-82108601;E-mail:liguiying@caas.cn
  • 作者简介:聂元冬,E-mail:dongdong20061552@126.com
  • 基金资助:

    国家自然科学基金(31060036,31271790)、国家“十一五”科技支撑计划(2009BADA7B01)

Expression of Soluble Acid Invertase Gene and Its Relationship with Sugar Accumulation in Sweet Sorghum Stem

 NIE  Yuan-Dong-1, ZHONG  Hai-Li-1, DUN Bao-Qing-1 , YE  Kai-2, WANG  Zhi-1, ZHU  Li-3, LI  Gui-Ying-1   

  1. 1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Biomass Energy Research Center/National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081
    2.Institute of Biomass Energy, Xinjiang Academy of Agricultural Sciences, Urumuqi 830091
    3.Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2013-05-15 Online:2013-11-01 Published:2013-05-15

摘要: 【目的】通过检测不同甜高粱品种在各个生育时期叶片和茎秆中SAI基因的差异表达量与含糖量,探讨二者之间的相关性,为研究甜高粱糖分积累机制提供理论依据。【方法】高效液相色谱(HPLC)测定甜高粱品种不同生育时期茎秆中的含糖量,qRT-PCR分析叶片和茎秆中SAI基因的表达水平。【结果】拔节期蔗糖含量很低,主要是还原性糖,抽穗和开花期蔗糖含量显著增加,灌浆和成熟期持续增加至最高值,成熟后又有所下降。高糖品种整个生育时期叶片中SAI基因的表达水平高于中糖、低糖和髓干型三类品种,茎秆中SAI基因的表达水平则相反。甜高粱成熟期糖分含量与叶片中SAI基因的表达量呈显著正相关关系,相关系数达0.7239,而与茎秆中的表达量呈负相关关系,相关系数为-0.5971。【结论】甜高粱茎秆蔗糖积累与SAI基因在叶片和茎秆中的表达水平具有相关性,叶片中SAI基因的表达与蔗糖积累正相关,茎秆中SAI基因表达与蔗糖积累负相关,叶片的相关系数高于茎秆。

关键词: 甜高粱 , 糖分积累 , SAI基因 , 表达分析

Abstract: 【Objective】To better understand the mechanism of sucrose accumulation in sweet sorghum stem, the correlation between sugar content and soluble acid invertase(SAI) gene expression was analyzed by monitoring the expression of SAI in leaves and stems and the sugar content in stems.【Method】The sugar content and expression of SAI gene was investigated by using high performance liquid chromatography (HPLC) and qRT-PCR respectively.【Result】Reducing sugar was the major component at the elongation stage, and sucrose level was lower, then sucrose content was increased significantly at heading and flowering stages, until reached the maximum level at milking and ripening stages, while it declined after ripening. The expression of SAI gene in high-sugar varieties was higher than mid, low, and pith dry varieties in leaves, while it was just the reverse in stems. The sugar content of sweet sorghum at ripening stage was significantly negatively correlated with the expression of SAI gene in stems (r=-0.5971), and was significantly positively correlated with the expression of SAI gene in leaves (r=0.7239).【Conclusion】Sucrose accumulation was correlated with the expression of SAI gene in leaves and stems: a significantly positive correlation between sucrose and the expression of SAI gene in leaves, while a significant negative correlation between sucrose and the expression of SAI gene in stems, the correlation coefficient with leaves was higher than with stem.

Key words: sweet sorghum , sugar accumulation , soluble acid invertase (SAI) gene , expression analysis