龙眼SPL基因家族全基因组鉴定及表达分析

doi:10.3864/j.issn.0578-1752.2020.20.014

摘要/Abstract

摘要：

【目的】鉴定龙眼SPL（DlSPL）基因家族所有成员,并对其成员进行表达分析,为探究SPL在龙眼生长发育中的功能研究提供参考。【方法】采用生物信息学方法对龙眼SPL基因家族成员进行鉴定,并对其基本理化性质、基因结构、系统进化关系、启动子顺式作用元件、FPKM值进行分析;利用qRT-PCR技术检测其在非胚性愈伤组织（nonembryonic callus,NEC）、胚性培养物及不同激素处理下的胚性愈伤组织（embryonic callus,EC）中的表达情况。【结果】DlSPL基因家族共有14个成员,其基因结构和蛋白结构均高度保守且只有一个SBP结构域,DlSPL启动子存在大量的光反应元件、组织特异性调控元件、激素应答元件、胁迫响应元件和植物生长发育有关的顺式调控元件。RNA-seq表达量分析（FPKM值）表明,11个DlSPL成员在不同光质处理的EC中表达,只有DlSPL8在蓝光和白光处理下的EC中呈下调表达趋势;11个DlSPL成员在激素2,4-D和KT中表达,其中DlSPL3和DlSPL13在2,4-D和激动素（kinetin,KT）共同处理下的表达量高于2,4-D和KT分开处理;有13个DlSPL成员在非胚性及胚性培养物中表达,其中7个成员在NEC阶段表达量最高。qRT-PCR结果表明,在不同胚性培养物中,DlSPL1和DlSPL14在EC阶段表达最高,DlSPL5、DlSPL7和DlSPL13在不完全胚性紧实结构（incomplete embryonic compact structure,ICpEC）阶段表达量最高;DlSPL1、DlSPL5、DlSPL7和DlSPL13成员响应脱落酸（abscisic acid,ABA）信号并显著下调;DlSPL5响应茉莉酸甲酯（methyl jasmonate,MeJA）信号且呈上调表达趋势,其他3个成员呈下调表达趋势。【结论】共鉴定出龙眼SPL基因家族成员14个,均含有一个高度保守的SBP结构域;DlSPL可能参与龙眼体胚的形态建成,并响应ABA和MeJA的应答。

关键词: 龙眼, SPL, 全基因组鉴定, 表达分析

Abstract:

【Objective】To provide a reference for the study of the function of SPL in Dimocarpus longan growth and development, all members of the longan SPL(DlSPL) gene family were identified and their expression patterns were analyzed in this study.【Method】The DlSPL family members, with their basic physicochemical properties, gene structures, phylogenetic relationships, and cis-acting promoter elements, were identified by bioinformatics analysis, and their expression patterns in non-embryonic callus (NEC), embryogenic culture and embryonic callus (EC) under different hormone treatments were analyzed by FPKM and qRT-PCR technology. 【Result】The results showed that there were 14 DlSPL gene family members, and their gene and protein structures were all highly conserved and contained only one SBP domain. The promoters of DlSPL genes had a large number of light response elements, stress response elements, hormone response elements, tissue specific regulatory elements and cis-regulatory elements related to plant growth and development, indicating that DlSPL genes might be regulated by light, hormone, stress and other factors. RNA-seq expression analysis showed that 11 of 14 DlSPL members were detected in EC under the different light qualities and different hormones treatments, and only DlSPL8 showed a down-regulated expression trend under blue and white light treatments, and the expression levels of DlSPL3 and DlSPL13 members were higher in EC under 2, 4-D+KT treatment than that of under 2, 4-D and KT treatment, respectively; 13 DlSPLs were detected in non-embryogenic and embryogenic cultures, seven members of them had the highest expression levels in the NEC stage. QRT-PCR results showed that DlSPL1 and DlSPL14 had the highest expression levels in the EC stage, DlSPL5, DlSPL7 and DlSPL13 had the highest expression levels in the incomplete embryonic compact structure (ICpEC) stage; DlSPL1, DlSPL5, DlSPL7, and DlSPL13 were down-regulated under abscisic acid treatments. DlSPL5 was up-regulated under methyl jasmonate acid treatments, while the other three members were down-regulated. 【Conclusion】 A total of 14 longan SPL gene family members contained a highly conserved SBP domain were identified, and the DlSPL gene family might play an important role in longan somatic embryos, and respond to abscisic acid and methyl jasmonate element treatments.

Key words: Dimocarpus longan, SPL, genome-wide identification, expression analysis

路保顺,朱永静,张舒婷,吕煜梦,李晓斐,宋雨洋,赖钟雄,林玉玲. 龙眼SPL基因家族全基因组鉴定及表达分析[J]. 中国农业科学, 2020, 53(20): 4259-4270.

LU BaoShun,ZHU YongJing,ZHANG ShuTing,LÜ YuMeng,LI XiaoFei,SONG YuYang,LAI ZhongXiong,LIN YuLing. Whole-Genome Identification and Expression Analysis of SPL Gene Family in Dimocarpus Longan[J]. Scientia Agricultura Sinica, 2020, 53(20): 4259-4270.

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