HMGCS1基因对猪肌肉生长发育的影响及 miRNA-18a/b对HMGCS1基因的调控

doi:10.3864/j.issn.0578-1752.2013.12.015

中国农业科学 ›› 2013, Vol. 46 ›› Issue (12): 2543-2549.doi: 10.3864/j.issn.0578-1752.2013.12.015

HMGCS1基因对猪肌肉生长发育的影响及 miRNA-18a/b对HMGCS1基因的调控

孙伟, 唐中林, 谭林, 雷初朝, 李奎

1.西北农林科技大学动物科技学院，陕西杨凌 712100
2.中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与利用重点开放实验室，北京 100193

收稿日期:2012-12-06 出版日期:2013-06-15 发布日期:2013-04-02
通讯作者: 通信作者唐中林，E-mail：zhonglinqy_99@sina.com。通信作者雷初朝，E-mail：leichuzhao1118@126.com
作者简介:孙伟，E-mail：25092596@qq.com
基金资助:
国家自然科学基金项目（31171192，31172182）、国家重大科学研究计划（2012CB124706-6）

Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig

SUN Wei, TANG Zhong-Lin, TAN Lin, LEI Chu-Chao, LI Kui

1.College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi
2.Institute of Animal Science, Chinese Academy of Agricultural Sciences/State Key Laboratory of Farm Animal Genetic Resources and Utilization, Beijing 100193

Received:2012-12-06 Online:2013-06-15 Published:2013-04-02

摘要/Abstract

摘要： 【目的】研究HMGCS1基因对猪骨骼肌生长发育的影响及其靶标的验证。【方法】采用real-time PCR对通城猪不同组织以及背最长肌不同发育时间点的HMGCS1基因的表达变化进行检测。【结果】①HMGCS1基因在成年猪肺组织中的表达量最高；②在背最长肌发育中，该基因分别在胚胎期33 和65 d高表达，出生后20、30、40和60 d低表达；③双荧光素酶试验显示，转染miR-18a/b minics组荧光素酶活性低于对照组。【结论】HMGCS1参与了猪骨骼肌生长发育过程，并受miR-18a/b的调控，是猪的产肉性状的潜在候选基因。

关键词: 猪 , HMGCS1基因 , miR-18a/b , 骨骼肌 , 生长发育

Abstract: 【Objective】The purpose of the present research was to explore whether HMGCS1 impacts the skeletal muscle growth and development of pig and validate its target gene.【Method】In this study，the expression of HMGCS1 in different tissues and the expression change during Longissimus dorsi muscle development (28 stages) was investigated by real-time qPCR.【Result】The result showed that the HMGCS1 gene was strongly expressed in lung of adult pig, and the expression level of HMGCS1 gene in embryonic period 33 d and 65 d were higher than those in other periods in Tongcheng pigs, and lower expression at postnatal 20 d, 30 d, 40 d and 60 d. The luciferase activity was lower in miR-18a/b minics than in the control.【Conclusion】The results of study indicated that HMGCS1 regulates skeletal muscle growth and development by miR-18a/b, and is a potential candidate target gene of meat production traits.

Key words: pig , HMGCS1 gene , miR-18a/b , skeletal muscle , growth and development

孙伟, 唐中林, 谭林, 雷初朝, 李奎. HMGCS1基因对猪肌肉生长发育的影响及 miRNA-18a/b对HMGCS1基因的调控[J]. 中国农业科学, 2013, 46(12): 2543-2549.

SUN Wei, TANG Zhong-Lin, TAN Lin, LEI Chu-Chao, LI Kui. Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig[J]. Scientia Agricultura Sinica, 2013, 46(12): 2543-2549.

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HMGCS1基因对猪肌肉生长发育的影响及 miRNA-18a/b对HMGCS1基因的调控

Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig

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