[1]Bingham E T. Haploids from cultivated alfalfa, Medicago sativa. Nature, 1969, 221(5183): 865- 866.[2]Bingham E T, McCoy T J. Cultivated alfalfa at the diploid level: origin, reproductive stability, and yield of seed and forage. Crop Science, 1979, 19(1): 97-100.[3]洪绂曾, 卢欣石, 高洪文. 苜蓿科学. 北京: 中国农业出版社, 2009: 186-215.Hong F Z, Lu X S, Gao H W. Alfalfa Science. Beijing: China Agriculture Press, 2009: 186-215. (in Chinese)[4]Bingham E T, Murphy R P. Breeding and morphological studies on multifoliolate selections of alfalfa, Medicago sativa L. Crop Science, 1964, 5(3): 233-235.[5]武自念, 魏臻武, 赵艳, 刘高军, 甘欣, 雷艳芳, 黎明. 多叶苜蓿自交一代遗传关系的SSR分析. 中国草地学报, 2010, 32(6): 27-32.Wu Z N, Wei Z W, Zhao Y, Liu G J, Gan X, Lei Y F, Li M. SSR analysis of genetic relationships on multifoliolate selfing alfalfa. Chinese Journal of Grassland, 2010, 32(6): 27-32. (in Chinese)[6]Palmer C C E D, Palmer C E, Keller W W A, Kasha K J. Haploids in Crop Improvement II. Springer-Verlag, 2005.[7]Ferrie A M R, Caswell K L. Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture, 2011, 104(3): 301-309.[8]Saunders J W, Bingham E T. Production of alfalfa plants from dallus tissue. Crop Science, 1972, 12(6): 804-808.[9]Zagorska N, Dimitrov B, Gadeva P, Robeva P. Regeneration and characterization of plants obtained from anther cultures in Medicago sativa L. In Vitro Cellular and Developmental Biology-Plan, 1997, 33(2): 107-110. [10]Zagorska N, Dimitrov B. Induced androgenes is in alfalfa(Medicago sativa L. Plant Cell Reports, 1995, 14(4): 249-252.[11]郭景文, 曹致中, 师尚礼, 刘彩萍. 四种豆科牧草花药和组织培养的研究. 甘肃农业大学学报, 1990, 25(2): 131-137.Guo J W, Cao Z Z, Shi S L, Liu C P. The anther and tissue culturing for four legumes. Journal of Gansu Agricultural University, 1990, 25(2): 131-137. (in Chinese)[12]郭景文. 四种豆科牧草花药及组织培养研究的总结. 青海草业, 1998, 7(2): 9-13.Guo J W. A Study on anther and tissue culture of four species of legumes. Qinghai Pratacuiture, 1998, 7(2): 9-13. (in Chinese)[13]徐速. 苜蓿花粉植株的诱导. 科学通报, 1981, 12: 756-758.Xu S. Induction of alfalfa pollen plantlets. Chinese Science Bulletin, 1981, 12: 756-758. (in Chinese)[14]黄远新, 玉永雄, 胡艳. 南方紫花苜蓿不同外植体离体培养的研究. 中国草地, 2003, 25(3): 42-47.Huang Y X, Yu Y X, Hu Y. Tissue culture of different explants of alfalfa cultivated in Southern China in vitro. Grassland of China, 2003, 25(3): 42-47. (in Chinese)[15]吴丽芳, 毕玉芬, 奎嘉祥. 紫花苜蓿花药愈伤组织和幼苗诱导技术的研究. 中国草地, 2005, 27(6): 28-33.Wu L F, Bi Y F, Kui J X. Study on induction technology of anther callus and seedlings of alfalfa. Grassland of China, 2005, 27(6): 28-33. (in Chinese)[16]耿小丽, 魏臻武, 程鹏武, 刘欢. 苜蓿花药培养及倍性鉴定. 草原与草坪, 2008, 130(5): 1-5.Geng X L, Wei Z W, Cheng P W, Liu H. Induction of anther and identification of cell chromosome ploidy of alfalfa. Grassland and Turf, 2008, 130(5): 1-5. (in Chinese)[17]耿小丽, 魏臻武, 姚喜红. 采用流式细胞仪鉴定紫花苜蓿花药愈伤组织的变异. 草业学报, 2011, 20(3): 156-161.Geng X L, Wei Z W, Yao X H. Genetic variation of medicago sativa callus revealed by the ploidy analyser. Prataculturae Sinica, 2011, 20(3): 156-161. (in Chinese)[18]高霞, 石凤翎, 伊风艳, 张宇. 苜蓿雄性不育系花药愈伤形成及分化培养条件的研究. 中国草地学报, 2012, 34(2): 41-46.Gao X, Shi F L, Yi F Y, Zhang Y. Study on the culture conditions for anther callus induction and differentiation of male sterile line of alfalfa. Chinese Journal of Grassland, 2012, 34(2): 41-46. (in Chinese)[19]姚喜红, 魏臻武, 耿小丽. 苜蓿花药悬浮培养体系的建立及其影响因素. 草地学报, 2010, 18(3): 358-364. Yao X H, Wei Z W, Geng X L. Establishment of alfalfa anther suspension culture system and the influencing factors. Acta Agrestia Sinica, 2010, 18(3): 358-364. (in Chinese)[20]李增红, 张博, 陈爱萍, 罗玉鹏. 不同培养基对苜蓿花药愈伤组织继代和分化的影响. 新疆农业科学, 2008, 45(5): 950-955.Li Z H, Zhang B, Chen A P, Luo Y P. Effect of different culture medium on callus subculture and differentiation from alfalfa anther. Xinjiang Agricultural Sciences, 2008, 45(5): 950-955. (in Chinese)[21]马菊兰, 张博. 低温预处理和低温预培养对苜蓿新品系花药愈伤组织诱导的影响. 中国草地学报, 2007, 29(6): 59-63.Ma J L, Zhang B. Effects of cold pretreatment and low temperature preculture on the callus induction rate on anther culture of alfalfa. Chinese Journal of Grassland, 2007, 29(6): 59-63. (in Chinese) [22]Gamborg O L, Miller R A, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 1968, 50(1): 151-158.[23]李海营, 乔桂荣, 刘明英, 蒋晶, 张玲, 卓仁英. 麻竹花药诱导再生植株的染色体倍性分析. 植物学报, 2011, 46(1): 74-78.Li H Y, Qiao G R, Liu M Y, Jiang J, Zhang L, Zhuo R Y. Analysis of ploidy in dedrocalamus latiflorus plants obtained by anther culture. Chinese Bulletin of Botany, 2011, 46(1): 74-78. (in Chinese) [24]Dpoole?el J, Binarová P, Lcretti S. Analysis of Nuclear DNA content in plant cells by flow cytometry. Biologia Plantarum, 1989, 31(2): 113-120. [25]耿小丽, 魏臻武, 姚喜红, 赵艳. 苜蓿花药培养再生植株染色体倍性检测研究. 草地学报, 2010, 18(5): 714-718.Geng X L, Wei Z W, Yao X H, Zhao Y. Anther culture of medicago sativa L. and ploidy detection. Acta Agrestia Sinica, 2010, 18(5): 714-718. (in Chinese)[26]Bolibok H, Rakoczy-Trojanowska M. Genetic mapping of QTLs for tissue-culture response in plants. Euphytica, 2006, 149(1/2): 73-83. |