人工合成高抗菌核病甘蓝型油菜几种关键酶编码基因的表达与其抗性的关系

doi:10.3864/j.issn.0578-1752.2012.22.001

中国农业科学 ›› 2012, Vol. 45 ›› Issue (22): 4543-4551.doi: 10.3864/j.issn.0578-1752.2012.22.001

• 作物遗传育种·种质资源·分子遗传学 • 下一篇

人工合成高抗菌核病甘蓝型油菜几种关键酶编码基因的表达与其抗性的关系

万华方, 刘, 瑶, 梅家琴, 丁一娟, 梁, 颖, 曲存民, 卢, 坤, 李加纳, 钱, 伟

西南大学农学与生物科技学院/教育部南方山地农业工程研究中心/重庆市油菜工程技术研究中心，重庆 400716

收稿日期:2012-09-04 出版日期:2012-11-15 发布日期:2012-10-08
通讯作者: 通信作者钱伟，Tel：023-68250701；Fax：023-68250701；E-mail：qianwei666@hotmail.com
作者简介:万华方，Tel：023-6260794；E-mail：wanhua05@163.com。刘瑶，E-mail：liuyao963@126.com。万华方和刘瑶为同等贡献作者。
基金资助:
中央高校基本科研业务费专项（XDJK2010C082）、国家自然科学基金项目（31171585）、高等学校学科创新工程计划“111”项目（B12006）

Relationship Between the Expression of Genes Encoding Resistance-related Enzymes and the Resistance to Sclerotinia sclerotiorum in Resynthesized Brassica napus with High Level of Resistance

WAN Hua-Fang, LIU , YAO , MEI Jia-Qin, DING Yi-Juan, LIANG , YING , QU Cun-Min, LU , KUN , LI Jia-Na, QIAN , WEI

College of Agronomy and Biotechnology, Southwest University/ Engineering Research Center of South Upland Agriculture, Ministry of Education/Chongqing Rapeseed Engineering and Technology Research Center, Chongqing 400716

Received:2012-09-04 Online:2012-11-15 Published:2012-10-08

摘要/Abstract

摘要： 【目的】研究人工合成高抗菌核病甘蓝型油菜RB165的几种关键酶编码基因与菌核病抗性的关系。【方法】以RB165及其野生甘蓝抗病亲本BP（Brassica incana）和耐、感病甘蓝型油菜为试材，离体鉴定叶、茎菌核病抗性，并用qRT-PCR对4个病程相关基因（OXO、Cu/Zn SOD、PR2和PR3）在叶片接种0、6、12、24、36 h的表达进行检测。【结果】参试材料叶片和茎杆的抗病性达到极显著正相关（r = 0.93）； RB165的抗病性低于RP，但极显著高于耐、感病品（种）系；接种核盘菌后，OXO的表达被抑制，Cu/Zn SOD的表达则先下降后上升，PR2的表达出现2次峰值，PR3的表达被剧烈诱导，4个基因在不同材料间的相对表达量差异极显著，但变化规律相似。【结论】RB165的抗病性强于耐病甘蓝型油菜，但几种酶编码基因受核盘菌的诱导表达与菌核病抗性没有直接关系。

关键词: 甘蓝, 人工合成甘蓝型油菜, 核盘菌, 病程相关基因, qRT-PCR

Abstract: 【Objective】 The relationship between the expression of genes encoding resistance-related enzymes and the resistance to S. sclerotiorum in resynthesized B. napus RB165 with high level of resistance was investigated.【Method】 RB165 was employed to investigate sclerotinia resistance using detached leaf and stem assay and to monitor the expression of genes encoding resistance-related enzymes (oxalate oxidase, Cu/Zn superoxidase dismutase, β-1,3-glucanase and chitinase) after sclerotinia inoculation with qRT-PCR, together with the resistant parental adopter (RP), four natural rapeseed lines with diverse levels of resistance.【Result】The leaf resistance was highly and positively correlated to that of the stem (r = 0.93) among 6 accessions. The resistance of RB165 was lower than that of RP, but significantly higher than that of natural rapeseed. The pattern of gene relative expression detected with qRT-PCR at 0, 6, 12, 24, 36 hours post inoculation (hpi) of S. sclerotiorum significantly differed in leaf, but the dynamics was similar among 6 accessions, i.e. OXO was inhibited, Cu/Zn SOD was inhibited at the beginning of inoculation, and then activated, PR2 was strongly induced twice and PR3 was dramatically activated. 【Conclusion】 RB165 was more resistant to S. sclerotiorum than natural rapeseed. No significant relationship was detected between S. sclerotiorum resistance and the expression of OXO, Cu/Zn SOD, PR2 and PR3.

Key words: Brassica incana, resynthesized Brassica napus, Sclerotinia sclerotiorum, pathogenesis related genes, qRT-PCR

万华方, 刘, 瑶, 梅家琴, 丁一娟, 梁, 颖, 曲存民, 卢, 坤, 李加纳, 钱, 伟. 人工合成高抗菌核病甘蓝型油菜几种关键酶编码基因的表达与其抗性的关系[J]. 中国农业科学, 2012, 45(22): 4543-4551.

WAN Hua-Fang, LIU , YAO , MEI Jia-Qin, DING Yi-Juan, LIANG , YING , QU Cun-Min, LU , KUN , LI Jia-Na, QIAN , WEI . Relationship Between the Expression of Genes Encoding Resistance-related Enzymes and the Resistance to Sclerotinia sclerotiorum in Resynthesized Brassica napus with High Level of Resistance[J]. Scientia Agricultura Sinica, 2012, 45(22): 4543-4551.

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人工合成高抗菌核病甘蓝型油菜几种关键酶编码基因的表达与其抗性的关系

Relationship Between the Expression of Genes Encoding Resistance-related Enzymes and the Resistance to Sclerotinia sclerotiorum in Resynthesized Brassica napus with High Level of Resistance

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