关键词: 甘蓝, 胞质不育源, 分子鉴定, CAPS标记

Abstract: 【Objective】 Two feasible Ogura cytoplasmic male sterility sources (CMS HY and CMSR3) in cabbage were used to identify its cytoplasmic DNA distinction. 【Method】 With chloroplast SSR (cpSSR) and mitochondrial SSR (mtSSR) primers, the technologies of polyacrylamide gel electrophoresis and DNA sequencing were used to explore the molecular differences between Ogura CMSHY and CMSR3. The markers with restriction enzyme recognition sites were converted into CAPS markers.【Result】 cpSSR amplicons could not differ two CMS sources on polyacrylamide gel, but the sequence of amplicon ACP9 differed them on SSR repeat numbers. In 21 mtSSR primers, only amplicon of mtSSR2 could distinguish two CMS sources on polyacrylamide gel. Moreover, nine polymorphic differences were obtained with sequencing in other 5 mtSSR primers. Two polymorphic differences with MseⅠ restriction enzyme recognition sites were converted into CAPS markers, named as m92-143 MseⅠ, m1-346 MseⅠ.【Conclusion】 Two cabbage Ogura CMS sources were distinguished with cpSSR and mtSSR markers. CAPS markers could be used as a fast, easy and accurate method to distinguish two CMS sources.

Key words: Brassica oleracea var. capitata L., Ogura CMS source, molecular distinction, CAPS (cleaved amplified polymorphic sequences)